Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04197 and H281DRAFT_04198 overlap by 8 nucleotides H281DRAFT_04198 and H281DRAFT_04199 are separated by 113 nucleotides H281DRAFT_04199 and H281DRAFT_04200 are separated by 53 nucleotides H281DRAFT_04200 and H281DRAFT_04201 are separated by 52 nucleotides
H281DRAFT_04197: H281DRAFT_04197 - hypothetical protein, at 315,928 to 317,103
_04197
H281DRAFT_04198: H281DRAFT_04198 - Protein of unknown function (DUF2946), at 317,096 to 317,668
_04198
H281DRAFT_04199: H281DRAFT_04199 - Putative Zn-dependent protease, contains TPR repeats, at 317,782 to 319,650
_04199
H281DRAFT_04200: H281DRAFT_04200 - cyclic pyranopterin monophosphate synthase subunit MoaC, at 319,704 to 320,180
_04200
H281DRAFT_04201: H281DRAFT_04201 - O-antigen ligase, at 320,233 to 322,050
_04201
Position (kb) 317
318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 316.792 kb on + strand, within H281DRAFT_04197 at 316.792 kb on + strand, within H281DRAFT_04197 at 316.793 kb on - strand, within H281DRAFT_04197 at 316.849 kb on + strand, within H281DRAFT_04197 at 316.850 kb on - strand, within H281DRAFT_04197 at 316.850 kb on - strand, within H281DRAFT_04197 at 317.188 kb on + strand, within H281DRAFT_04198 at 317.273 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.325 kb on + strand, within H281DRAFT_04198 at 317.327 kb on + strand, within H281DRAFT_04198 at 317.327 kb on + strand, within H281DRAFT_04198 at 317.327 kb on + strand, within H281DRAFT_04198 at 317.327 kb on + strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand, within H281DRAFT_04198 at 317.328 kb on - strand at 317.342 kb on + strand, within H281DRAFT_04198 at 317.367 kb on + strand, within H281DRAFT_04198 at 317.783 kb on + strand at 317.861 kb on + strand at 317.861 kb on + strand at 317.862 kb on - strand at 317.862 kb on - strand at 317.864 kb on + strand at 317.865 kb on - strand at 318.002 kb on + strand, within H281DRAFT_04199 at 318.002 kb on + strand, within H281DRAFT_04199 at 318.069 kb on + strand, within H281DRAFT_04199 at 318.097 kb on + strand, within H281DRAFT_04199 at 318.098 kb on - strand, within H281DRAFT_04199 at 318.308 kb on + strand, within H281DRAFT_04199 at 318.344 kb on + strand, within H281DRAFT_04199 at 318.344 kb on + strand, within H281DRAFT_04199 at 318.633 kb on - strand, within H281DRAFT_04199 at 318.641 kb on + strand, within H281DRAFT_04199 at 318.642 kb on - strand, within H281DRAFT_04199 at 318.642 kb on - strand, within H281DRAFT_04199 at 318.728 kb on + strand, within H281DRAFT_04199 at 318.728 kb on + strand, within H281DRAFT_04199 at 318.728 kb on + strand, within H281DRAFT_04199 at 318.729 kb on - strand, within H281DRAFT_04199 at 318.825 kb on - strand, within H281DRAFT_04199 at 318.825 kb on - strand, within H281DRAFT_04199 at 318.861 kb on + strand, within H281DRAFT_04199 at 319.040 kb on + strand, within H281DRAFT_04199 at 319.040 kb on + strand, within H281DRAFT_04199 at 319.041 kb on - strand, within H281DRAFT_04199 at 319.041 kb on - strand, within H281DRAFT_04199 at 319.096 kb on + strand, within H281DRAFT_04199 at 319.097 kb on - strand, within H281DRAFT_04199 at 319.180 kb on - strand, within H281DRAFT_04199 at 319.328 kb on + strand, within H281DRAFT_04199 at 319.380 kb on - strand, within H281DRAFT_04199 at 319.588 kb on + strand at 319.588 kb on + strand at 319.589 kb on - strand at 319.593 kb on + strand at 319.614 kb on + strand at 319.614 kb on + strand at 319.615 kb on - strand at 319.615 kb on - strand at 319.674 kb on + strand at 319.674 kb on + strand at 319.675 kb on - strand at 319.675 kb on - strand at 319.679 kb on + strand at 319.680 kb on - strand at 320.216 kb on + strand at 320.217 kb on - strand at 320.345 kb on + strand at 320.346 kb on - strand at 320.351 kb on + strand at 320.351 kb on + strand at 320.636 kb on + strand, within H281DRAFT_04201
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 316,792 + H281DRAFT_04197 0.73 -0.6 316,792 + H281DRAFT_04197 0.73 +0.7 316,793 - H281DRAFT_04197 0.74 -0.7 316,849 + H281DRAFT_04197 0.78 -0.7 316,850 - H281DRAFT_04197 0.78 -0.0 316,850 - H281DRAFT_04197 0.78 -0.2 317,188 + H281DRAFT_04198 0.16 -0.7 317,273 + H281DRAFT_04198 0.31 +0.3 317,325 + H281DRAFT_04198 0.40 +0.6 317,325 + H281DRAFT_04198 0.40 +0.0 317,325 + H281DRAFT_04198 0.40 -0.4 317,325 + H281DRAFT_04198 0.40 +0.2 317,325 + H281DRAFT_04198 0.40 +0.4 317,325 + H281DRAFT_04198 0.40 -0.6 317,327 + H281DRAFT_04198 0.40 -0.2 317,327 + H281DRAFT_04198 0.40 +0.5 317,327 + H281DRAFT_04198 0.40 -0.3 317,327 + H281DRAFT_04198 0.40 -0.3 317,328 - H281DRAFT_04198 0.40 +1.3 317,328 - H281DRAFT_04198 0.40 +0.8 317,328 - H281DRAFT_04198 0.40 +0.3 317,328 - H281DRAFT_04198 0.40 -0.3 317,328 - H281DRAFT_04198 0.40 -0.1 317,328 - H281DRAFT_04198 0.40 +0.7 317,328 - H281DRAFT_04198 0.40 +0.4 317,328 - +4.0 317,342 + H281DRAFT_04198 0.43 +1.4 317,367 + H281DRAFT_04198 0.47 -0.2 317,783 + +0.7 317,861 + +0.1 317,861 + +0.2 317,862 - +0.2 317,862 - -0.1 317,864 + -0.5 317,865 - -1.3 318,002 + H281DRAFT_04199 0.12 +1.7 318,002 + H281DRAFT_04199 0.12 -0.8 318,069 + H281DRAFT_04199 0.15 +0.1 318,097 + H281DRAFT_04199 0.17 +0.6 318,098 - H281DRAFT_04199 0.17 +0.1 318,308 + H281DRAFT_04199 0.28 -0.8 318,344 + H281DRAFT_04199 0.30 +0.2 318,344 + H281DRAFT_04199 0.30 -1.1 318,633 - H281DRAFT_04199 0.46 +1.1 318,641 + H281DRAFT_04199 0.46 +0.9 318,642 - H281DRAFT_04199 0.46 +2.6 318,642 - H281DRAFT_04199 0.46 +0.4 318,728 + H281DRAFT_04199 0.51 -0.7 318,728 + H281DRAFT_04199 0.51 +1.0 318,728 + H281DRAFT_04199 0.51 +0.9 318,729 - H281DRAFT_04199 0.51 -0.3 318,825 - H281DRAFT_04199 0.56 -0.4 318,825 - H281DRAFT_04199 0.56 -0.1 318,861 + H281DRAFT_04199 0.58 +0.4 319,040 + H281DRAFT_04199 0.67 -0.6 319,040 + H281DRAFT_04199 0.67 -0.7 319,041 - H281DRAFT_04199 0.67 +1.4 319,041 - H281DRAFT_04199 0.67 -1.3 319,096 + H281DRAFT_04199 0.70 -0.3 319,097 - H281DRAFT_04199 0.70 -1.0 319,180 - H281DRAFT_04199 0.75 +0.6 319,328 + H281DRAFT_04199 0.83 -1.8 319,380 - H281DRAFT_04199 0.86 -1.2 319,588 + +0.1 319,588 + +0.3 319,589 - +1.3 319,593 + +0.6 319,614 + +0.1 319,614 + +0.6 319,615 - -0.5 319,615 - -2.0 319,674 + -0.1 319,674 + +4.4 319,675 - -0.3 319,675 - +0.5 319,679 + +1.1 319,680 - -2.3 320,216 + -0.7 320,217 - -0.1 320,345 + -1.0 320,346 - +0.9 320,351 + -1.0 320,351 + -0.5 320,636 + H281DRAFT_04201 0.22 +1.3
Or see this region's nucleotide sequence