Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04114 and H281DRAFT_04115 are separated by 114 nucleotides H281DRAFT_04115 and H281DRAFT_04116 are separated by 5 nucleotides
H281DRAFT_04114: H281DRAFT_04114 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 224,779 to 225,537
_04114
H281DRAFT_04115: H281DRAFT_04115 - Regulator of sirC expression, contains transglutaminase-like and TPR domains, at 225,652 to 226,494
_04115
H281DRAFT_04116: H281DRAFT_04116 - putative peptidoglycan lipid II flippase, at 226,500 to 228,050
_04116
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 224.718 kb on + strand at 224.724 kb on + strand at 224.725 kb on - strand at 224.750 kb on + strand at 224.750 kb on + strand at 224.751 kb on - strand at 224.751 kb on - strand at 224.751 kb on - strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.798 kb on + strand at 224.798 kb on + strand at 224.799 kb on - strand at 224.808 kb on + strand at 225.223 kb on + strand, within H281DRAFT_04114 at 225.224 kb on - strand, within H281DRAFT_04114 at 225.251 kb on - strand, within H281DRAFT_04114 at 225.445 kb on + strand, within H281DRAFT_04114 at 225.445 kb on + strand, within H281DRAFT_04114 at 225.535 kb on + strand at 225.536 kb on - strand at 225.536 kb on - strand at 225.610 kb on - strand at 225.653 kb on + strand at 225.654 kb on - strand at 225.666 kb on + strand at 225.666 kb on + strand at 225.779 kb on + strand, within H281DRAFT_04115 at 225.841 kb on + strand, within H281DRAFT_04115 at 225.841 kb on + strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.843 kb on + strand at 225.843 kb on + strand, within H281DRAFT_04115 at 225.843 kb on + strand, within H281DRAFT_04115 at 225.844 kb on - strand, within H281DRAFT_04115 at 225.896 kb on + strand, within H281DRAFT_04115 at 225.896 kb on + strand, within H281DRAFT_04115 at 225.921 kb on + strand, within H281DRAFT_04115 at 225.921 kb on + strand, within H281DRAFT_04115 at 225.922 kb on - strand, within H281DRAFT_04115 at 226.163 kb on + strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.241 kb on + strand, within H281DRAFT_04115 at 226.241 kb on + strand, within H281DRAFT_04115 at 226.475 kb on + strand at 226.523 kb on - strand at 226.528 kb on + strand at 226.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 224,718 + +0.1 224,724 + -0.0 224,725 - -0.4 224,750 + +0.8 224,750 + +0.7 224,751 - +0.1 224,751 - -1.8 224,751 - +0.3 224,754 + +0.3 224,754 + +0.8 224,754 + -0.7 224,754 + -0.1 224,754 + -1.3 224,755 - +1.1 224,755 - -0.0 224,755 - +0.3 224,755 - +0.1 224,755 - +0.8 224,755 - -0.1 224,755 - -0.1 224,798 + +0.3 224,798 + +0.1 224,799 - +0.0 224,808 + -0.4 225,223 + H281DRAFT_04114 0.58 +0.0 225,224 - H281DRAFT_04114 0.59 +0.1 225,251 - H281DRAFT_04114 0.62 -0.7 225,445 + H281DRAFT_04114 0.88 -1.3 225,445 + H281DRAFT_04114 0.88 -1.4 225,535 + +0.7 225,536 - +1.0 225,536 - +0.3 225,610 - -1.4 225,653 + -0.3 225,654 - +0.1 225,666 + +1.2 225,666 + -0.5 225,779 + H281DRAFT_04115 0.15 +0.6 225,841 + H281DRAFT_04115 0.22 -2.7 225,841 + H281DRAFT_04115 0.22 -1.2 225,842 - H281DRAFT_04115 0.23 -0.6 225,842 - H281DRAFT_04115 0.23 +0.0 225,842 - H281DRAFT_04115 0.23 +0.2 225,843 + +1.0 225,843 + H281DRAFT_04115 0.23 +0.2 225,843 + H281DRAFT_04115 0.23 -1.5 225,844 - H281DRAFT_04115 0.23 +0.1 225,896 + H281DRAFT_04115 0.29 -3.0 225,896 + H281DRAFT_04115 0.29 -2.3 225,921 + H281DRAFT_04115 0.32 +1.4 225,921 + H281DRAFT_04115 0.32 -1.8 225,922 - H281DRAFT_04115 0.32 +0.3 226,163 + H281DRAFT_04115 0.61 +0.2 226,239 - H281DRAFT_04115 0.70 +0.1 226,239 - H281DRAFT_04115 0.70 +1.1 226,239 - H281DRAFT_04115 0.70 +0.4 226,239 - H281DRAFT_04115 0.70 -0.3 226,239 - H281DRAFT_04115 0.70 -0.0 226,241 + H281DRAFT_04115 0.70 +0.1 226,241 + H281DRAFT_04115 0.70 -1.0 226,475 + -0.5 226,523 - +0.6 226,528 + +1.4 226,528 + +1.6
Or see this region's nucleotide sequence