Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04069 and H281DRAFT_04070 are separated by 64 nucleotides H281DRAFT_04070 and H281DRAFT_04071 overlap by 1 nucleotides H281DRAFT_04071 and H281DRAFT_04072 are separated by 79 nucleotides
H281DRAFT_04069: H281DRAFT_04069 - L-aspartate oxidase, at 169,390 to 170,988
_04069
H281DRAFT_04070: H281DRAFT_04070 - nicotinate-nucleotide pyrophosphorylase [carboxylating], at 171,053 to 171,982
_04070
H281DRAFT_04071: H281DRAFT_04071 - quinolinate synthetase, at 171,982 to 173,169
_04071
H281DRAFT_04072: H281DRAFT_04072 - stearoyl-CoA desaturase (delta-9 desaturase), at 173,249 to 174,406
_04072
Position (kb) 171
172
173
174 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 171.035 kb on + strand at 171.036 kb on - strand at 171.072 kb on + strand at 171.408 kb on + strand, within H281DRAFT_04070 at 171.409 kb on - strand, within H281DRAFT_04070 at 171.454 kb on - strand, within H281DRAFT_04070 at 171.514 kb on + strand, within H281DRAFT_04070 at 171.534 kb on + strand, within H281DRAFT_04070 at 171.564 kb on - strand, within H281DRAFT_04070 at 171.602 kb on - strand, within H281DRAFT_04070 at 171.886 kb on - strand, within H281DRAFT_04070 at 171.936 kb on + strand at 172.004 kb on + strand at 172.005 kb on - strand at 172.005 kb on - strand at 172.054 kb on - strand at 172.077 kb on + strand at 172.077 kb on + strand at 172.077 kb on + strand at 172.077 kb on + strand at 172.077 kb on + strand at 172.078 kb on - strand at 172.078 kb on - strand at 172.135 kb on - strand, within H281DRAFT_04071 at 172.179 kb on + strand, within H281DRAFT_04071 at 172.234 kb on - strand, within H281DRAFT_04071 at 172.234 kb on - strand, within H281DRAFT_04071 at 172.279 kb on - strand, within H281DRAFT_04071 at 172.279 kb on - strand, within H281DRAFT_04071 at 172.533 kb on - strand at 172.533 kb on - strand, within H281DRAFT_04071 at 172.720 kb on + strand, within H281DRAFT_04071 at 172.721 kb on - strand, within H281DRAFT_04071 at 172.937 kb on + strand, within H281DRAFT_04071 at 172.937 kb on + strand, within H281DRAFT_04071 at 172.937 kb on + strand, within H281DRAFT_04071 at 172.937 kb on + strand, within H281DRAFT_04071 at 172.938 kb on - strand, within H281DRAFT_04071 at 172.938 kb on - strand, within H281DRAFT_04071 at 172.938 kb on - strand, within H281DRAFT_04071 at 172.938 kb on - strand, within H281DRAFT_04071 at 172.940 kb on + strand, within H281DRAFT_04071 at 172.940 kb on + strand, within H281DRAFT_04071 at 172.940 kb on + strand, within H281DRAFT_04071 at 172.940 kb on + strand, within H281DRAFT_04071 at 172.941 kb on - strand, within H281DRAFT_04071 at 172.941 kb on - strand, within H281DRAFT_04071 at 173.155 kb on + strand at 173.155 kb on + strand at 173.156 kb on - strand at 173.223 kb on + strand at 173.250 kb on + strand at 173.250 kb on + strand at 173.251 kb on - strand at 173.406 kb on + strand, within H281DRAFT_04072 at 173.521 kb on - strand, within H281DRAFT_04072
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 171,035 + +0.1 171,036 - -0.8 171,072 + -1.5 171,408 + H281DRAFT_04070 0.38 -0.4 171,409 - H281DRAFT_04070 0.38 -2.6 171,454 - H281DRAFT_04070 0.43 -1.9 171,514 + H281DRAFT_04070 0.50 -0.7 171,534 + H281DRAFT_04070 0.52 -0.7 171,564 - H281DRAFT_04070 0.55 -1.2 171,602 - H281DRAFT_04070 0.59 -1.5 171,886 - H281DRAFT_04070 0.90 -1.4 171,936 + -0.9 172,004 + -3.1 172,005 - -1.5 172,005 - -2.9 172,054 - -1.3 172,077 + -0.8 172,077 + -1.0 172,077 + -1.1 172,077 + -3.2 172,077 + -4.5 172,078 - -1.3 172,078 - -1.9 172,135 - H281DRAFT_04071 0.13 -0.9 172,179 + H281DRAFT_04071 0.17 -0.8 172,234 - H281DRAFT_04071 0.21 -2.5 172,234 - H281DRAFT_04071 0.21 -1.3 172,279 - H281DRAFT_04071 0.25 -1.0 172,279 - H281DRAFT_04071 0.25 -1.8 172,533 - +1.0 172,533 - H281DRAFT_04071 0.46 -1.5 172,720 + H281DRAFT_04071 0.62 -1.0 172,721 - H281DRAFT_04071 0.62 -1.1 172,937 + H281DRAFT_04071 0.80 -4.3 172,937 + H281DRAFT_04071 0.80 -1.0 172,937 + H281DRAFT_04071 0.80 -1.6 172,937 + H281DRAFT_04071 0.80 -0.2 172,938 - H281DRAFT_04071 0.80 -1.0 172,938 - H281DRAFT_04071 0.80 -1.4 172,938 - H281DRAFT_04071 0.80 -3.4 172,938 - H281DRAFT_04071 0.80 -0.8 172,940 + H281DRAFT_04071 0.81 +1.5 172,940 + H281DRAFT_04071 0.81 -1.1 172,940 + H281DRAFT_04071 0.81 -1.8 172,940 + H281DRAFT_04071 0.81 -3.4 172,941 - H281DRAFT_04071 0.81 -1.2 172,941 - H281DRAFT_04071 0.81 -1.8 173,155 + -3.4 173,155 + -1.5 173,156 - +0.3 173,223 + -4.6 173,250 + +0.1 173,250 + +0.3 173,251 - +0.5 173,406 + H281DRAFT_04072 0.14 -1.0 173,521 - H281DRAFT_04072 0.23 +2.6
Or see this region's nucleotide sequence