Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04036 and H281DRAFT_04037 are separated by 62 nucleotides H281DRAFT_04037 and H281DRAFT_04038 are separated by 50 nucleotides H281DRAFT_04038 and H281DRAFT_04039 are separated by 54 nucleotides H281DRAFT_04039 and H281DRAFT_04040 are separated by 221 nucleotides
H281DRAFT_04036: H281DRAFT_04036 - tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33), at 136,533 to 137,321
_04036
H281DRAFT_04037: H281DRAFT_04037 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 137,384 to 138,235
_04037
H281DRAFT_04038: H281DRAFT_04038 - Protein of unknown function (DUF1439), at 138,286 to 138,876
_04038
H281DRAFT_04039: H281DRAFT_04039 - hypothetical protein, at 138,931 to 139,251
_04039
H281DRAFT_04040: H281DRAFT_04040 - oxygen-independent coproporphyrinogen-3 oxidase, at 139,473 to 140,858
_04040
Position (kb)
138
139 Strain fitness (log2 ratio)
-1
0
1
2 at 137.319 kb on + strand at 137.320 kb on - strand at 137.386 kb on - strand at 137.410 kb on + strand at 138.321 kb on + strand at 138.322 kb on - strand at 138.322 kb on - strand at 138.336 kb on + strand at 138.348 kb on - strand, within H281DRAFT_04038 at 138.351 kb on + strand, within H281DRAFT_04038 at 138.352 kb on - strand, within H281DRAFT_04038 at 138.352 kb on - strand, within H281DRAFT_04038 at 138.388 kb on - strand, within H281DRAFT_04038 at 138.388 kb on - strand, within H281DRAFT_04038 at 138.389 kb on + strand, within H281DRAFT_04038 at 138.390 kb on - strand, within H281DRAFT_04038 at 138.409 kb on + strand, within H281DRAFT_04038 at 138.409 kb on + strand, within H281DRAFT_04038 at 138.410 kb on - strand, within H281DRAFT_04038 at 138.453 kb on + strand, within H281DRAFT_04038 at 138.453 kb on + strand, within H281DRAFT_04038 at 138.454 kb on - strand, within H281DRAFT_04038 at 138.455 kb on + strand, within H281DRAFT_04038 at 138.455 kb on + strand, within H281DRAFT_04038 at 138.455 kb on + strand, within H281DRAFT_04038 at 138.455 kb on + strand, within H281DRAFT_04038 at 138.456 kb on - strand, within H281DRAFT_04038 at 138.676 kb on + strand, within H281DRAFT_04038 at 138.676 kb on + strand, within H281DRAFT_04038 at 138.677 kb on - strand, within H281DRAFT_04038 at 138.749 kb on + strand, within H281DRAFT_04038 at 138.750 kb on - strand, within H281DRAFT_04038 at 138.876 kb on + strand at 138.876 kb on + strand at 138.876 kb on + strand at 138.877 kb on - strand at 138.877 kb on - strand at 138.923 kb on + strand at 138.924 kb on - strand at 138.924 kb on - strand at 138.933 kb on - strand at 138.933 kb on - strand at 139.161 kb on + strand, within H281DRAFT_04039 at 139.252 kb on + strand at 139.252 kb on + strand at 139.252 kb on + strand at 139.252 kb on + strand at 139.253 kb on - strand at 139.265 kb on + strand at 139.283 kb on - strand at 139.283 kb on - strand at 139.283 kb on - strand at 139.359 kb on - strand at 139.518 kb on + strand at 139.540 kb on - strand at 139.540 kb on - strand at 139.541 kb on + strand at 139.541 kb on + strand at 139.541 kb on + strand at 139.541 kb on + strand at 139.542 kb on - strand at 139.542 kb on - strand at 139.542 kb on - strand at 139.542 kb on - strand at 139.542 kb on - strand at 139.548 kb on + strand at 139.549 kb on - strand at 139.593 kb on + strand at 139.593 kb on + strand at 139.598 kb on - strand at 139.598 kb on - strand at 139.644 kb on + strand, within H281DRAFT_04040 at 139.645 kb on - strand, within H281DRAFT_04040 at 139.652 kb on + strand, within H281DRAFT_04040 at 139.652 kb on + strand, within H281DRAFT_04040 at 139.653 kb on - strand, within H281DRAFT_04040 at 139.680 kb on + strand, within H281DRAFT_04040 at 139.795 kb on + strand, within H281DRAFT_04040
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 137,319 + +0.7 137,320 - +0.5 137,386 - -0.5 137,410 + -0.1 138,321 + -0.2 138,322 - -0.7 138,322 - +0.4 138,336 + -1.7 138,348 - H281DRAFT_04038 0.10 +1.4 138,351 + H281DRAFT_04038 0.11 -0.6 138,352 - H281DRAFT_04038 0.11 +1.3 138,352 - H281DRAFT_04038 0.11 -0.0 138,388 - H281DRAFT_04038 0.17 +0.3 138,388 - H281DRAFT_04038 0.17 +0.4 138,389 + H281DRAFT_04038 0.17 +0.8 138,390 - H281DRAFT_04038 0.18 +0.7 138,409 + H281DRAFT_04038 0.21 +0.4 138,409 + H281DRAFT_04038 0.21 -0.5 138,410 - H281DRAFT_04038 0.21 +1.3 138,453 + H281DRAFT_04038 0.28 +0.5 138,453 + H281DRAFT_04038 0.28 +0.3 138,454 - H281DRAFT_04038 0.28 +0.8 138,455 + H281DRAFT_04038 0.29 +0.8 138,455 + H281DRAFT_04038 0.29 -0.5 138,455 + H281DRAFT_04038 0.29 +0.7 138,455 + H281DRAFT_04038 0.29 +0.2 138,456 - H281DRAFT_04038 0.29 +0.6 138,676 + H281DRAFT_04038 0.66 +0.2 138,676 + H281DRAFT_04038 0.66 +1.0 138,677 - H281DRAFT_04038 0.66 -0.6 138,749 + H281DRAFT_04038 0.78 -0.6 138,750 - H281DRAFT_04038 0.79 +0.0 138,876 + +0.4 138,876 + +2.0 138,876 + +0.8 138,877 - -0.1 138,877 - +0.3 138,923 + -0.3 138,924 - +0.2 138,924 - -0.2 138,933 - +0.2 138,933 - -0.4 139,161 + H281DRAFT_04039 0.72 +0.6 139,252 + -0.8 139,252 + -0.9 139,252 + +0.8 139,252 + -0.3 139,253 - +0.4 139,265 + -1.1 139,283 - +0.6 139,283 - -0.6 139,283 - -0.0 139,359 - +0.0 139,518 + +0.2 139,540 - +0.3 139,540 - -0.4 139,541 + -0.9 139,541 + -0.5 139,541 + -1.1 139,541 + -0.0 139,542 - +1.3 139,542 - +0.0 139,542 - -0.5 139,542 - -0.2 139,542 - +0.6 139,548 + -0.0 139,549 - +0.7 139,593 + -0.8 139,593 + +0.6 139,598 - -0.5 139,598 - -1.7 139,644 + H281DRAFT_04040 0.12 -0.0 139,645 - H281DRAFT_04040 0.12 -1.0 139,652 + H281DRAFT_04040 0.13 +2.2 139,652 + H281DRAFT_04040 0.13 +0.6 139,653 - H281DRAFT_04040 0.13 +0.7 139,680 + H281DRAFT_04040 0.15 -0.0 139,795 + H281DRAFT_04040 0.23 -0.2
Or see this region's nucleotide sequence