Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03523 and H281DRAFT_03524 are separated by 111 nucleotides H281DRAFT_03524 and H281DRAFT_03525 are separated by 218 nucleotides H281DRAFT_03525 and H281DRAFT_03526 are separated by 111 nucleotides H281DRAFT_03526 and H281DRAFT_03527 are separated by 269 nucleotides H281DRAFT_03527 and H281DRAFT_03528 are separated by 39 nucleotides
H281DRAFT_03523: H281DRAFT_03523 - mannose-6-phosphate isomerase, at 22,288 to 23,397
_03523
H281DRAFT_03524: H281DRAFT_03524 - hypothetical protein, at 23,509 to 23,784
_03524
H281DRAFT_03525: H281DRAFT_03525 - cold-shock DNA-binding protein family, at 24,003 to 24,206
_03525
H281DRAFT_03526: H281DRAFT_03526 - methyl-accepting chemotaxis sensory transducer with TarH sensor, at 24,318 to 25,940
_03526
H281DRAFT_03527: H281DRAFT_03527 - uncharacterized domain 1-containing protein, at 26,210 to 26,644
_03527
H281DRAFT_03528: H281DRAFT_03528 - ureidoglycolate lyase, at 26,684 to 27,532
_03528
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 23.328 kb on - strand at 23.359 kb on + strand at 23.359 kb on + strand at 23.360 kb on - strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.413 kb on + strand at 23.414 kb on - strand at 23.513 kb on + strand at 23.514 kb on - strand at 23.514 kb on - strand at 23.514 kb on - strand at 23.514 kb on - strand at 23.514 kb on - strand at 23.565 kb on + strand, within H281DRAFT_03524 at 23.565 kb on + strand, within H281DRAFT_03524 at 23.566 kb on - strand, within H281DRAFT_03524 at 23.567 kb on + strand, within H281DRAFT_03524 at 23.568 kb on - strand, within H281DRAFT_03524 at 23.568 kb on - strand, within H281DRAFT_03524 at 23.568 kb on - strand, within H281DRAFT_03524 at 23.587 kb on - strand, within H281DRAFT_03524 at 23.758 kb on - strand at 23.774 kb on + strand at 23.774 kb on + strand at 23.774 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.805 kb on + strand at 23.881 kb on + strand at 23.889 kb on + strand at 23.889 kb on + strand at 23.912 kb on + strand at 23.912 kb on + strand at 23.923 kb on + strand at 23.923 kb on + strand at 23.923 kb on + strand at 23.924 kb on - strand at 23.924 kb on - strand at 23.924 kb on - strand at 23.924 kb on - strand at 23.961 kb on + strand at 23.961 kb on + strand at 23.979 kb on + strand at 23.979 kb on + strand at 23.979 kb on + strand at 23.980 kb on - strand at 23.980 kb on - strand at 23.980 kb on - strand at 23.991 kb on - strand at 24.015 kb on - strand at 24.016 kb on - strand at 24.029 kb on + strand, within H281DRAFT_03525 at 24.119 kb on + strand, within H281DRAFT_03525 at 24.120 kb on - strand, within H281DRAFT_03525 at 24.204 kb on + strand at 24.204 kb on + strand at 24.204 kb on + strand at 24.208 kb on + strand at 24.208 kb on + strand at 24.209 kb on - strand at 24.209 kb on - strand at 24.217 kb on + strand at 24.217 kb on + strand at 24.234 kb on - strand at 24.248 kb on - strand at 24.250 kb on + strand at 24.827 kb on + strand, within H281DRAFT_03526 at 24.827 kb on + strand, within H281DRAFT_03526 at 24.864 kb on - strand, within H281DRAFT_03526 at 24.915 kb on + strand, within H281DRAFT_03526 at 24.915 kb on + strand, within H281DRAFT_03526 at 24.915 kb on + strand, within H281DRAFT_03526 at 24.916 kb on - strand, within H281DRAFT_03526 at 24.916 kb on - strand, within H281DRAFT_03526 at 25.302 kb on + strand, within H281DRAFT_03526 at 25.323 kb on - strand, within H281DRAFT_03526 at 25.323 kb on - strand, within H281DRAFT_03526 at 25.323 kb on - strand, within H281DRAFT_03526 at 25.469 kb on - strand, within H281DRAFT_03526 at 25.570 kb on + strand, within H281DRAFT_03526 at 25.863 kb on + strand at 25.864 kb on - strand at 25.975 kb on - strand at 25.975 kb on - strand at 25.975 kb on - strand at 26.035 kb on + strand at 26.036 kb on - strand at 26.099 kb on + strand at 26.139 kb on - strand at 26.148 kb on - strand at 26.221 kb on - strand at 26.379 kb on - strand, within H281DRAFT_03527 at 26.379 kb on - strand, within H281DRAFT_03527 at 26.448 kb on - strand, within H281DRAFT_03527 at 26.448 kb on - strand, within H281DRAFT_03527 at 26.460 kb on - strand, within H281DRAFT_03527 at 26.510 kb on + strand, within H281DRAFT_03527 at 26.510 kb on + strand, within H281DRAFT_03527 at 26.511 kb on - strand, within H281DRAFT_03527 at 26.697 kb on + strand at 26.698 kb on - strand at 26.763 kb on + strand at 26.763 kb on + strand at 26.764 kb on - strand at 26.856 kb on + strand, within H281DRAFT_03528 at 26.856 kb on + strand, within H281DRAFT_03528 at 26.856 kb on + strand, within H281DRAFT_03528 at 26.856 kb on + strand, within H281DRAFT_03528 at 26.856 kb on + strand, within H281DRAFT_03528 at 26.857 kb on - strand, within H281DRAFT_03528
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 23,328 - +0.7 23,359 + -2.6 23,359 + +0.7 23,360 - -0.9 23,413 + +0.0 23,413 + -0.2 23,413 + -0.3 23,413 + -0.7 23,413 + -1.2 23,413 + -0.8 23,413 + -1.3 23,414 - -1.6 23,513 + +0.3 23,514 - +0.4 23,514 - -0.7 23,514 - -1.4 23,514 - -2.2 23,514 - +3.6 23,565 + H281DRAFT_03524 0.20 -0.8 23,565 + H281DRAFT_03524 0.20 +0.4 23,566 - H281DRAFT_03524 0.21 -1.0 23,567 + H281DRAFT_03524 0.21 -1.6 23,568 - H281DRAFT_03524 0.21 +0.2 23,568 - H281DRAFT_03524 0.21 -0.9 23,568 - H281DRAFT_03524 0.21 -0.5 23,587 - H281DRAFT_03524 0.28 -1.6 23,758 - -1.7 23,774 + -0.0 23,774 + -0.8 23,774 + +0.0 23,805 + +0.4 23,805 + +0.4 23,805 + -0.5 23,805 + -0.6 23,805 + -1.8 23,805 + -0.6 23,805 + -0.7 23,805 + -1.4 23,881 + -2.5 23,889 + -0.6 23,889 + -0.3 23,912 + -0.9 23,912 + -1.3 23,923 + -0.9 23,923 + -0.4 23,923 + +0.2 23,924 - +1.6 23,924 - -1.2 23,924 - -1.7 23,924 - -2.4 23,961 + -0.9 23,961 + -1.0 23,979 + -1.6 23,979 + -1.5 23,979 + -1.5 23,980 - -1.0 23,980 - +0.5 23,980 - -0.2 23,991 - -1.4 24,015 - -0.9 24,016 - -1.3 24,029 + H281DRAFT_03525 0.13 -0.6 24,119 + H281DRAFT_03525 0.57 +1.9 24,120 - H281DRAFT_03525 0.57 -1.3 24,204 + -1.9 24,204 + -1.4 24,204 + -0.8 24,208 + -1.3 24,208 + -0.7 24,209 - -1.9 24,209 - -0.3 24,217 + -2.1 24,217 + -0.1 24,234 - -0.7 24,248 - -0.5 24,250 + -2.0 24,827 + H281DRAFT_03526 0.31 -1.8 24,827 + H281DRAFT_03526 0.31 -0.8 24,864 - H281DRAFT_03526 0.34 +0.0 24,915 + H281DRAFT_03526 0.37 -0.2 24,915 + H281DRAFT_03526 0.37 +1.2 24,915 + H281DRAFT_03526 0.37 -0.7 24,916 - H281DRAFT_03526 0.37 -0.1 24,916 - H281DRAFT_03526 0.37 -1.1 25,302 + H281DRAFT_03526 0.61 +0.6 25,323 - H281DRAFT_03526 0.62 +0.4 25,323 - H281DRAFT_03526 0.62 -1.2 25,323 - H281DRAFT_03526 0.62 +3.3 25,469 - H281DRAFT_03526 0.71 -0.1 25,570 + H281DRAFT_03526 0.77 +0.1 25,863 + +0.8 25,864 - +1.1 25,975 - -1.0 25,975 - -0.5 25,975 - +0.6 26,035 + +0.9 26,036 - -0.3 26,099 + +0.5 26,139 - -0.2 26,148 - -0.6 26,221 - +0.1 26,379 - H281DRAFT_03527 0.39 +0.0 26,379 - H281DRAFT_03527 0.39 +0.1 26,448 - H281DRAFT_03527 0.55 -0.4 26,448 - H281DRAFT_03527 0.55 +0.8 26,460 - H281DRAFT_03527 0.57 -0.9 26,510 + H281DRAFT_03527 0.69 +0.5 26,510 + H281DRAFT_03527 0.69 -1.4 26,511 - H281DRAFT_03527 0.69 -0.2 26,697 + +1.1 26,698 - -0.5 26,763 + -0.1 26,763 + -0.2 26,764 - -0.6 26,856 + H281DRAFT_03528 0.20 -0.2 26,856 + H281DRAFT_03528 0.20 -0.2 26,856 + H281DRAFT_03528 0.20 -1.8 26,856 + H281DRAFT_03528 0.20 +0.0 26,856 + H281DRAFT_03528 0.20 -0.4 26,857 - H281DRAFT_03528 0.20 -0.2
Or see this region's nucleotide sequence