Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03517 and H281DRAFT_03518 are separated by 197 nucleotides H281DRAFT_03518 and H281DRAFT_03519 are separated by 439 nucleotides H281DRAFT_03519 and H281DRAFT_03520 are separated by 370 nucleotides
H281DRAFT_03517: H281DRAFT_03517 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 17,538 to 18,413
_03517
H281DRAFT_03518: H281DRAFT_03518 - hypothetical protein, at 18,611 to 18,916
_03518
H281DRAFT_03519: H281DRAFT_03519 - hypothetical protein, at 19,356 to 19,514
_03519
H281DRAFT_03520: H281DRAFT_03520 - hypothetical protein, at 19,885 to 21,225
_03520
Position (kb)
19
20 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 18.435 kb on + strand at 18.436 kb on - strand at 18.436 kb on - strand at 18.438 kb on + strand at 18.438 kb on + strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.495 kb on - strand at 18.509 kb on + strand at 18.612 kb on + strand at 18.613 kb on - strand at 18.613 kb on - strand at 18.663 kb on + strand, within H281DRAFT_03518 at 18.663 kb on + strand, within H281DRAFT_03518 at 18.720 kb on + strand, within H281DRAFT_03518 at 18.724 kb on - strand, within H281DRAFT_03518 at 18.787 kb on - strand, within H281DRAFT_03518 at 18.916 kb on + strand at 18.916 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 19.090 kb on - strand at 19.172 kb on - strand at 19.193 kb on + strand at 19.224 kb on + strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.227 kb on - strand at 19.354 kb on + strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.357 kb on + strand at 19.357 kb on + strand at 19.432 kb on + strand, within H281DRAFT_03519 at 19.438 kb on + strand, within H281DRAFT_03519 at 19.439 kb on - strand, within H281DRAFT_03519 at 19.531 kb on + strand at 19.532 kb on - strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.608 kb on - strand at 19.608 kb on - strand at 19.664 kb on - strand at 19.664 kb on - strand at 19.743 kb on + strand at 19.744 kb on - strand at 19.744 kb on - strand at 19.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 18,435 + -0.2 18,436 - +0.4 18,436 - -0.0 18,438 + -0.0 18,438 + +0.1 18,439 - -0.7 18,439 - +0.9 18,439 - -0.4 18,495 - +0.6 18,509 + -1.3 18,612 + -0.6 18,613 - -0.3 18,613 - +0.8 18,663 + H281DRAFT_03518 0.17 -0.8 18,663 + H281DRAFT_03518 0.17 -0.0 18,720 + H281DRAFT_03518 0.36 -0.8 18,724 - H281DRAFT_03518 0.37 -0.0 18,787 - H281DRAFT_03518 0.58 -2.4 18,916 + +0.2 18,916 + +0.7 18,916 + -1.0 18,917 - +1.3 18,917 - +0.0 18,917 - -2.1 18,917 - +0.2 19,090 - +0.2 19,172 - +0.4 19,193 + +1.0 19,224 + +0.2 19,225 - -0.2 19,225 - +1.1 19,225 - +0.1 19,226 + +0.0 19,226 + -0.2 19,226 + -0.2 19,227 - +0.1 19,354 + -0.2 19,355 - +0.4 19,355 - -0.6 19,355 - -0.2 19,355 - +0.2 19,357 + -0.8 19,357 + -0.8 19,432 + H281DRAFT_03519 0.48 +0.4 19,438 + H281DRAFT_03519 0.52 -0.4 19,439 - H281DRAFT_03519 0.52 -0.2 19,531 + +0.6 19,532 - +0.1 19,607 + +0.5 19,607 + +2.6 19,607 + +0.1 19,607 + -0.2 19,608 - +0.7 19,608 - +1.0 19,664 - -0.5 19,664 - +0.6 19,743 + +0.7 19,744 - +1.4 19,744 - +1.3 19,744 - +0.5
Or see this region's nucleotide sequence