Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03515 and H281DRAFT_03516 are separated by 37 nucleotides H281DRAFT_03516 and H281DRAFT_03517 are separated by 153 nucleotides H281DRAFT_03517 and H281DRAFT_03518 are separated by 197 nucleotides H281DRAFT_03518 and H281DRAFT_03519 are separated by 439 nucleotides
H281DRAFT_03515: H281DRAFT_03515 - polyhydroxyalkanoate depolymerase, intracellular, at 15,272 to 16,735
_03515
H281DRAFT_03516: H281DRAFT_03516 - NADH-FMN oxidoreductase RutF, flavin reductase (DIM6/NTAB) family, at 16,773 to 17,384
_03516
H281DRAFT_03517: H281DRAFT_03517 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 17,538 to 18,413
_03517
H281DRAFT_03518: H281DRAFT_03518 - hypothetical protein, at 18,611 to 18,916
_03518
H281DRAFT_03519: H281DRAFT_03519 - hypothetical protein, at 19,356 to 19,514
_03519
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 16.645 kb on - strand at 16.888 kb on + strand, within H281DRAFT_03516 at 16.888 kb on + strand, within H281DRAFT_03516 at 16.889 kb on - strand, within H281DRAFT_03516 at 16.933 kb on + strand, within H281DRAFT_03516 at 16.933 kb on + strand, within H281DRAFT_03516 at 16.934 kb on - strand, within H281DRAFT_03516 at 16.934 kb on - strand, within H281DRAFT_03516 at 16.961 kb on + strand, within H281DRAFT_03516 at 16.962 kb on - strand, within H281DRAFT_03516 at 17.251 kb on + strand, within H281DRAFT_03516 at 17.368 kb on + strand at 17.414 kb on - strand at 17.476 kb on - strand at 17.540 kb on - strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.606 kb on - strand at 18.102 kb on - strand, within H281DRAFT_03517 at 18.102 kb on - strand, within H281DRAFT_03517 at 18.116 kb on + strand, within H281DRAFT_03517 at 18.116 kb on + strand, within H281DRAFT_03517 at 18.151 kb on + strand, within H281DRAFT_03517 at 18.435 kb on + strand at 18.436 kb on - strand at 18.436 kb on - strand at 18.438 kb on + strand at 18.438 kb on + strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.495 kb on - strand at 18.509 kb on + strand at 18.612 kb on + strand at 18.613 kb on - strand at 18.613 kb on - strand at 18.663 kb on + strand, within H281DRAFT_03518 at 18.663 kb on + strand, within H281DRAFT_03518 at 18.720 kb on + strand, within H281DRAFT_03518 at 18.724 kb on - strand, within H281DRAFT_03518 at 18.787 kb on - strand, within H281DRAFT_03518 at 18.916 kb on + strand at 18.916 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 19.090 kb on - strand at 19.172 kb on - strand at 19.193 kb on + strand at 19.224 kb on + strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.227 kb on - strand at 19.354 kb on + strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.357 kb on + strand at 19.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 16,645 - +0.4 16,888 + H281DRAFT_03516 0.19 +0.2 16,888 + H281DRAFT_03516 0.19 -0.2 16,889 - H281DRAFT_03516 0.19 -0.9 16,933 + H281DRAFT_03516 0.26 -0.1 16,933 + H281DRAFT_03516 0.26 -0.4 16,934 - H281DRAFT_03516 0.26 +0.3 16,934 - H281DRAFT_03516 0.26 -0.6 16,961 + H281DRAFT_03516 0.31 +0.7 16,962 - H281DRAFT_03516 0.31 +0.7 17,251 + H281DRAFT_03516 0.78 +0.1 17,368 + +1.2 17,414 - +0.7 17,476 - -0.0 17,540 - +0.2 17,605 + +0.6 17,605 + +0.2 17,605 + +0.5 17,605 + -1.2 17,605 + +1.3 17,606 - +0.3 18,102 - H281DRAFT_03517 0.64 +0.2 18,102 - H281DRAFT_03517 0.64 +0.1 18,116 + H281DRAFT_03517 0.66 -1.2 18,116 + H281DRAFT_03517 0.66 -1.3 18,151 + H281DRAFT_03517 0.70 -0.2 18,435 + -0.2 18,436 - +0.4 18,436 - -0.0 18,438 + -0.0 18,438 + +0.1 18,439 - -0.7 18,439 - +0.9 18,439 - -0.4 18,495 - +0.6 18,509 + -1.3 18,612 + -0.6 18,613 - -0.3 18,613 - +0.8 18,663 + H281DRAFT_03518 0.17 -0.8 18,663 + H281DRAFT_03518 0.17 -0.0 18,720 + H281DRAFT_03518 0.36 -0.8 18,724 - H281DRAFT_03518 0.37 -0.0 18,787 - H281DRAFT_03518 0.58 -2.4 18,916 + +0.2 18,916 + +0.7 18,916 + -1.0 18,917 - +1.3 18,917 - +0.0 18,917 - -2.1 18,917 - +0.2 19,090 - +0.2 19,172 - +0.4 19,193 + +1.0 19,224 + +0.2 19,225 - -0.2 19,225 - +1.1 19,225 - +0.1 19,226 + +0.0 19,226 + -0.2 19,226 + -0.2 19,227 - +0.1 19,354 + -0.2 19,355 - +0.4 19,355 - -0.6 19,355 - -0.2 19,355 - +0.2 19,357 + -0.8 19,357 + -0.8
Or see this region's nucleotide sequence