Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03364 and H281DRAFT_03365 overlap by 4 nucleotides H281DRAFT_03365 and H281DRAFT_03366 overlap by 4 nucleotides H281DRAFT_03366 and H281DRAFT_03367 are separated by 22 nucleotides
H281DRAFT_03364: H281DRAFT_03364 - Crotonobetainyl-CoA:carnitine CoA-transferase CaiB, at 424,597 to 425,820
_03364
H281DRAFT_03365: H281DRAFT_03365 - choline dehydrogenase, at 425,817 to 427,457
_03365
H281DRAFT_03366: H281DRAFT_03366 - electron transfer flavoprotein beta subunit, at 427,454 to 428,203
_03366
H281DRAFT_03367: H281DRAFT_03367 - electron transfer flavoprotein alpha subunit apoprotein, at 428,226 to 429,170
_03367
Position (kb) 425
426
427
428 Strain fitness (log2 ratio) -1
0
1
2 at 425.005 kb on + strand, within H281DRAFT_03364 at 425.162 kb on + strand, within H281DRAFT_03364 at 425.199 kb on + strand, within H281DRAFT_03364 at 425.320 kb on + strand, within H281DRAFT_03364 at 425.320 kb on + strand, within H281DRAFT_03364 at 425.342 kb on - strand, within H281DRAFT_03364 at 425.512 kb on + strand, within H281DRAFT_03364 at 425.567 kb on - strand, within H281DRAFT_03364 at 425.713 kb on - strand at 425.727 kb on + strand at 425.750 kb on - strand at 425.750 kb on - strand at 426.134 kb on + strand, within H281DRAFT_03365 at 426.482 kb on + strand, within H281DRAFT_03365 at 426.482 kb on + strand, within H281DRAFT_03365 at 426.708 kb on + strand, within H281DRAFT_03365 at 426.829 kb on - strand, within H281DRAFT_03365 at 427.138 kb on - strand, within H281DRAFT_03365 at 427.198 kb on - strand, within H281DRAFT_03365 at 427.438 kb on - strand at 428.251 kb on + strand at 428.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 425,005 + H281DRAFT_03364 0.33 +0.8 425,162 + H281DRAFT_03364 0.46 -0.9 425,199 + H281DRAFT_03364 0.49 -0.8 425,320 + H281DRAFT_03364 0.59 +1.8 425,320 + H281DRAFT_03364 0.59 -1.2 425,342 - H281DRAFT_03364 0.61 +0.8 425,512 + H281DRAFT_03364 0.75 +0.8 425,567 - H281DRAFT_03364 0.79 +0.3 425,713 - +0.3 425,727 + +0.0 425,750 - -0.0 425,750 - +0.8 426,134 + H281DRAFT_03365 0.19 -0.5 426,482 + H281DRAFT_03365 0.41 +0.6 426,482 + H281DRAFT_03365 0.41 +0.2 426,708 + H281DRAFT_03365 0.54 -0.2 426,829 - H281DRAFT_03365 0.62 +1.9 427,138 - H281DRAFT_03365 0.80 +2.0 427,198 - H281DRAFT_03365 0.84 +0.4 427,438 - +0.0 428,251 + -0.0 428,294 + +0.8
Or see this region's nucleotide sequence