Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03304 and H281DRAFT_03305 are separated by 282 nucleotides H281DRAFT_03305 and H281DRAFT_03306 are separated by 31 nucleotides H281DRAFT_03306 and H281DRAFT_03307 overlap by 4 nucleotides H281DRAFT_03307 and H281DRAFT_03308 overlap by 28 nucleotides
H281DRAFT_03304: H281DRAFT_03304 - amidohydrolase, PncC family, at 367,456 to 367,989
_03304
H281DRAFT_03305: H281DRAFT_03305 - Acetyltransferases, at 368,272 to 368,781
_03305
H281DRAFT_03306: H281DRAFT_03306 - Cytochrome c, mono- and diheme variants, at 368,813 to 370,117
_03306
H281DRAFT_03307: H281DRAFT_03307 - Cytochrome c oxidase assembly factor CtaG, at 370,114 to 371,082
_03307
H281DRAFT_03308: H281DRAFT_03308 - hypothetical protein, at 371,055 to 371,387
_03308
Position (kb)
368
369
370
371 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 368.588 kb on + strand at 368.589 kb on - strand, within H281DRAFT_03305 at 368.690 kb on - strand, within H281DRAFT_03305 at 368.690 kb on - strand, within H281DRAFT_03305 at 368.690 kb on - strand, within H281DRAFT_03305 at 369.069 kb on - strand, within H281DRAFT_03306 at 369.718 kb on - strand, within H281DRAFT_03306 at 369.907 kb on + strand, within H281DRAFT_03306 at 370.217 kb on + strand, within H281DRAFT_03307 at 370.740 kb on + strand, within H281DRAFT_03307 at 371.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 368,588 + +0.8 368,589 - H281DRAFT_03305 0.62 +0.6 368,690 - H281DRAFT_03305 0.82 +0.2 368,690 - H281DRAFT_03305 0.82 +0.5 368,690 - H281DRAFT_03305 0.82 +0.8 369,069 - H281DRAFT_03306 0.20 -4.4 369,718 - H281DRAFT_03306 0.69 -0.4 369,907 + H281DRAFT_03306 0.84 -1.4 370,217 + H281DRAFT_03307 0.11 +0.9 370,740 + H281DRAFT_03307 0.65 -0.0 371,076 - +0.2
Or see this region's nucleotide sequence