Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02970 and H281DRAFT_02971 overlap by 4 nucleotides H281DRAFT_02971 and H281DRAFT_02972 are separated by 146 nucleotides H281DRAFT_02972 and H281DRAFT_02973 are separated by 428 nucleotides
H281DRAFT_02970: H281DRAFT_02970 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 584,309 to 585,730
_02970
H281DRAFT_02971: H281DRAFT_02971 - Protein of unknown function (DUF3225), at 585,727 to 586,107
_02971
H281DRAFT_02972: H281DRAFT_02972 - hypothetical protein, at 586,254 to 586,850
_02972
H281DRAFT_02973: H281DRAFT_02973 - C-terminal, D2-small domain-containing protein, of ClpB protein, at 587,279 to 589,102
_02973
Position (kb)
586
587 Strain fitness (log2 ratio)
-1
0
1 at 585.410 kb on + strand, within H281DRAFT_02970 at 585.964 kb on - strand, within H281DRAFT_02971 at 585.967 kb on + strand, within H281DRAFT_02971 at 586.256 kb on - strand at 586.372 kb on + strand, within H281DRAFT_02972 at 586.372 kb on + strand, within H281DRAFT_02972 at 586.477 kb on - strand, within H281DRAFT_02972 at 586.865 kb on + strand at 587.074 kb on - strand at 587.074 kb on - strand at 587.074 kb on - strand at 587.204 kb on + strand at 587.204 kb on + strand at 587.278 kb on + strand at 587.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 585,410 + H281DRAFT_02970 0.77 +0.1 585,964 - H281DRAFT_02971 0.62 -0.9 585,967 + H281DRAFT_02971 0.63 -0.2 586,256 - +1.0 586,372 + H281DRAFT_02972 0.20 -1.4 586,372 + H281DRAFT_02972 0.20 -1.6 586,477 - H281DRAFT_02972 0.37 +0.1 586,865 + +0.5 587,074 - -0.7 587,074 - -0.7 587,074 - +0.8 587,204 + -0.2 587,204 + -1.2 587,278 + +0.4 587,456 + +1.3
Or see this region's nucleotide sequence