Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02623 and H281DRAFT_02624 overlap by 4 nucleotides H281DRAFT_02624 and H281DRAFT_02625 are separated by 18 nucleotides H281DRAFT_02625 and H281DRAFT_02626 are separated by 243 nucleotides
H281DRAFT_02623: H281DRAFT_02623 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 199,338 to 200,678
_02623
H281DRAFT_02624: H281DRAFT_02624 - 4-carboxymuconolactone decarboxylase, at 200,675 to 201,229
_02624
H281DRAFT_02625: H281DRAFT_02625 - amino acid ABC transporter substrate-binding protein, PAAT family, at 201,248 to 202,153
_02625
H281DRAFT_02626: H281DRAFT_02626 - hypothetical protein, at 202,397 to 203,149
_02626
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1 at 200.325 kb on + strand, within H281DRAFT_02623 at 201.083 kb on - strand, within H281DRAFT_02624 at 201.083 kb on - strand, within H281DRAFT_02624 at 201.360 kb on + strand, within H281DRAFT_02625 at 201.421 kb on + strand, within H281DRAFT_02625 at 201.422 kb on - strand, within H281DRAFT_02625 at 201.552 kb on + strand, within H281DRAFT_02625 at 201.552 kb on + strand, within H281DRAFT_02625 at 201.553 kb on - strand, within H281DRAFT_02625 at 201.553 kb on - strand, within H281DRAFT_02625 at 201.614 kb on - strand, within H281DRAFT_02625 at 201.617 kb on - strand, within H281DRAFT_02625 at 201.689 kb on + strand, within H281DRAFT_02625 at 201.986 kb on - strand, within H281DRAFT_02625 at 202.153 kb on + strand at 202.153 kb on + strand at 202.154 kb on - strand at 202.179 kb on - strand at 202.306 kb on + strand at 202.307 kb on - strand at 202.649 kb on + strand, within H281DRAFT_02626 at 202.650 kb on - strand, within H281DRAFT_02626 at 203.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 200,325 + H281DRAFT_02623 0.74 +1.3 201,083 - H281DRAFT_02624 0.74 +0.8 201,083 - H281DRAFT_02624 0.74 +0.4 201,360 + H281DRAFT_02625 0.12 +0.1 201,421 + H281DRAFT_02625 0.19 +0.7 201,422 - H281DRAFT_02625 0.19 -1.0 201,552 + H281DRAFT_02625 0.34 +0.2 201,552 + H281DRAFT_02625 0.34 +1.0 201,553 - H281DRAFT_02625 0.34 -1.5 201,553 - H281DRAFT_02625 0.34 +0.9 201,614 - H281DRAFT_02625 0.40 +0.1 201,617 - H281DRAFT_02625 0.41 -1.0 201,689 + H281DRAFT_02625 0.49 +0.6 201,986 - H281DRAFT_02625 0.81 +0.4 202,153 + +0.3 202,153 + +0.7 202,154 - -0.5 202,179 - +1.4 202,306 + +0.5 202,307 - +0.2 202,649 + H281DRAFT_02626 0.33 -0.3 202,650 - H281DRAFT_02626 0.34 -0.5 203,127 - +0.1
Or see this region's nucleotide sequence