Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02321 and H281DRAFT_02322 are separated by 243 nucleotides H281DRAFT_02322 and H281DRAFT_02323 are separated by 90 nucleotides H281DRAFT_02323 and H281DRAFT_02324 are separated by 114 nucleotides
H281DRAFT_02321: H281DRAFT_02321 - Site-specific recombinase XerD, at 477,045 to 478,256
_02321
H281DRAFT_02322: H281DRAFT_02322 - hypothetical protein, at 478,500 to 478,661
_02322
H281DRAFT_02323: H281DRAFT_02323 - hypothetical protein, at 478,752 to 479,096
_02323
H281DRAFT_02324: H281DRAFT_02324 - pyruvate dehydrogenase E1 component, at 479,211 to 481,946
_02324
Position (kb) 478
479 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 477.580 kb on - strand, within H281DRAFT_02321 at 477.585 kb on + strand, within H281DRAFT_02321 at 477.776 kb on - strand, within H281DRAFT_02321 at 477.866 kb on + strand, within H281DRAFT_02321 at 477.867 kb on - strand, within H281DRAFT_02321 at 477.867 kb on - strand, within H281DRAFT_02321 at 477.883 kb on - strand, within H281DRAFT_02321 at 477.962 kb on - strand, within H281DRAFT_02321 at 477.962 kb on - strand, within H281DRAFT_02321 at 477.962 kb on - strand, within H281DRAFT_02321 at 477.962 kb on - strand, within H281DRAFT_02321 at 477.962 kb on - strand, within H281DRAFT_02321 at 478.277 kb on + strand at 478.277 kb on + strand at 478.278 kb on - strand at 478.278 kb on - strand at 478.278 kb on - strand at 478.307 kb on + strand at 478.385 kb on + strand at 478.386 kb on - strand at 478.461 kb on - strand at 478.522 kb on - strand, within H281DRAFT_02322 at 478.522 kb on - strand, within H281DRAFT_02322 at 478.746 kb on + strand at 478.747 kb on - strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.754 kb on - strand at 478.754 kb on - strand at 478.772 kb on + strand at 478.772 kb on + strand at 478.773 kb on - strand at 478.773 kb on - strand at 478.777 kb on + strand at 478.777 kb on + strand at 478.783 kb on + strand at 478.783 kb on + strand at 478.813 kb on + strand, within H281DRAFT_02323 at 478.814 kb on - strand, within H281DRAFT_02323 at 478.834 kb on + strand, within H281DRAFT_02323 at 478.840 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.895 kb on - strand, within H281DRAFT_02323 at 478.909 kb on + strand, within H281DRAFT_02323 at 478.924 kb on + strand, within H281DRAFT_02323 at 478.925 kb on - strand, within H281DRAFT_02323 at 479.002 kb on - strand, within H281DRAFT_02323 at 479.002 kb on - strand, within H281DRAFT_02323 at 479.085 kb on + strand at 479.086 kb on - strand at 479.151 kb on + strand at 479.151 kb on + strand at 479.151 kb on + strand at 479.152 kb on - strand at 479.167 kb on + strand at 479.178 kb on + strand at 479.178 kb on + strand at 479.179 kb on - strand at 479.307 kb on + strand at 479.431 kb on + strand at 479.432 kb on - strand at 479.464 kb on + strand at 479.518 kb on + strand, within H281DRAFT_02324 at 479.518 kb on + strand, within H281DRAFT_02324 at 479.518 kb on + strand, within H281DRAFT_02324 at 479.519 kb on - strand, within H281DRAFT_02324
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 477,580 - H281DRAFT_02321 0.44 +0.5 477,585 + H281DRAFT_02321 0.45 +0.5 477,776 - H281DRAFT_02321 0.60 -1.9 477,866 + H281DRAFT_02321 0.68 +0.5 477,867 - H281DRAFT_02321 0.68 -2.2 477,867 - H281DRAFT_02321 0.68 +0.2 477,883 - H281DRAFT_02321 0.69 -1.3 477,962 - H281DRAFT_02321 0.76 -1.2 477,962 - H281DRAFT_02321 0.76 +0.5 477,962 - H281DRAFT_02321 0.76 -0.1 477,962 - H281DRAFT_02321 0.76 +0.7 477,962 - H281DRAFT_02321 0.76 -0.2 478,277 + -0.5 478,277 + -0.1 478,278 - +0.1 478,278 - -0.2 478,278 - +0.1 478,307 + -0.1 478,385 + -0.2 478,386 - -0.4 478,461 - -0.3 478,522 - H281DRAFT_02322 0.14 +0.3 478,522 - H281DRAFT_02322 0.14 +0.7 478,746 + -0.1 478,747 - -1.2 478,753 + -0.0 478,753 + +0.2 478,753 + +0.8 478,753 + +2.1 478,753 + +0.3 478,754 - -0.1 478,754 - -0.3 478,772 + +0.7 478,772 + +0.7 478,773 - +1.5 478,773 - -0.3 478,777 + -0.1 478,777 + +0.2 478,783 + +0.6 478,783 + -0.6 478,813 + H281DRAFT_02323 0.18 +0.0 478,814 - H281DRAFT_02323 0.18 +0.8 478,834 + H281DRAFT_02323 0.24 +0.2 478,840 + H281DRAFT_02323 0.26 -0.2 478,879 + H281DRAFT_02323 0.37 +0.4 478,879 + H281DRAFT_02323 0.37 +0.8 478,879 + H281DRAFT_02323 0.37 -1.4 478,895 - H281DRAFT_02323 0.41 +0.1 478,909 + H281DRAFT_02323 0.46 +0.8 478,924 + H281DRAFT_02323 0.50 -0.2 478,925 - H281DRAFT_02323 0.50 +0.9 479,002 - H281DRAFT_02323 0.72 +0.3 479,002 - H281DRAFT_02323 0.72 -1.3 479,085 + -0.2 479,086 - +3.3 479,151 + +0.1 479,151 + +0.1 479,151 + -0.0 479,152 - +0.8 479,167 + +0.4 479,178 + -0.6 479,178 + -1.7 479,179 - -0.1 479,307 + -0.2 479,431 + -0.4 479,432 - -2.0 479,464 + +0.4 479,518 + H281DRAFT_02324 0.11 -1.7 479,518 + H281DRAFT_02324 0.11 +0.9 479,518 + H281DRAFT_02324 0.11 -0.4 479,519 - H281DRAFT_02324 0.11 +0.1
Or see this region's nucleotide sequence