Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02321

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02320 and H281DRAFT_02321 are separated by 169 nucleotidesH281DRAFT_02321 and H281DRAFT_02322 are separated by 243 nucleotidesH281DRAFT_02322 and H281DRAFT_02323 are separated by 90 nucleotidesH281DRAFT_02323 and H281DRAFT_02324 are separated by 114 nucleotides H281DRAFT_02320: H281DRAFT_02320 - tRNA modification GTPase trmE, at 475,481 to 476,875 _02320 H281DRAFT_02321: H281DRAFT_02321 - Site-specific recombinase XerD, at 477,045 to 478,256 _02321 H281DRAFT_02322: H281DRAFT_02322 - hypothetical protein, at 478,500 to 478,661 _02322 H281DRAFT_02323: H281DRAFT_02323 - hypothetical protein, at 478,752 to 479,096 _02323 H281DRAFT_02324: H281DRAFT_02324 - pyruvate dehydrogenase E1 component, at 479,211 to 481,946 _02324 Position (kb) 477 478 479Strain fitness (log2 ratio) -2 -1 0 1 2 3at 476.874 kb on - strandat 476.898 kb on - strandat 477.017 kb on + strandat 477.018 kb on - strandat 477.018 kb on - strandat 477.018 kb on - strandat 477.030 kb on - strandat 477.112 kb on - strandat 477.238 kb on + strand, within H281DRAFT_02321at 477.352 kb on + strand, within H281DRAFT_02321at 477.352 kb on + strand, within H281DRAFT_02321at 477.352 kb on + strand, within H281DRAFT_02321at 477.353 kb on - strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.373 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.375 kb on + strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.376 kb on - strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.377 kb on + strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.378 kb on - strand, within H281DRAFT_02321at 477.580 kb on - strand, within H281DRAFT_02321at 477.585 kb on + strand, within H281DRAFT_02321at 477.776 kb on - strand, within H281DRAFT_02321at 477.866 kb on + strand, within H281DRAFT_02321at 477.867 kb on - strand, within H281DRAFT_02321at 477.867 kb on - strand, within H281DRAFT_02321at 477.883 kb on - strand, within H281DRAFT_02321at 477.962 kb on - strand, within H281DRAFT_02321at 477.962 kb on - strand, within H281DRAFT_02321at 477.962 kb on - strand, within H281DRAFT_02321at 477.962 kb on - strand, within H281DRAFT_02321at 477.962 kb on - strand, within H281DRAFT_02321at 478.277 kb on + strandat 478.277 kb on + strandat 478.278 kb on - strandat 478.278 kb on - strandat 478.278 kb on - strandat 478.307 kb on + strandat 478.385 kb on + strandat 478.386 kb on - strandat 478.461 kb on - strandat 478.522 kb on - strand, within H281DRAFT_02322at 478.522 kb on - strand, within H281DRAFT_02322at 478.746 kb on + strandat 478.747 kb on - strandat 478.753 kb on + strandat 478.753 kb on + strandat 478.753 kb on + strandat 478.753 kb on + strandat 478.753 kb on + strandat 478.754 kb on - strandat 478.754 kb on - strandat 478.772 kb on + strandat 478.772 kb on + strandat 478.773 kb on - strandat 478.773 kb on - strandat 478.777 kb on + strandat 478.777 kb on + strandat 478.783 kb on + strandat 478.783 kb on + strandat 478.813 kb on + strand, within H281DRAFT_02323at 478.814 kb on - strand, within H281DRAFT_02323at 478.834 kb on + strand, within H281DRAFT_02323at 478.840 kb on + strand, within H281DRAFT_02323at 478.879 kb on + strand, within H281DRAFT_02323at 478.879 kb on + strand, within H281DRAFT_02323at 478.879 kb on + strand, within H281DRAFT_02323at 478.895 kb on - strand, within H281DRAFT_02323at 478.909 kb on + strand, within H281DRAFT_02323at 478.924 kb on + strand, within H281DRAFT_02323at 478.925 kb on - strand, within H281DRAFT_02323at 479.002 kb on - strand, within H281DRAFT_02323at 479.002 kb on - strand, within H281DRAFT_02323at 479.085 kb on + strandat 479.086 kb on - strandat 479.151 kb on + strandat 479.151 kb on + strandat 479.151 kb on + strandat 479.152 kb on - strandat 479.167 kb on + strandat 479.178 kb on + strandat 479.178 kb on + strandat 479.179 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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476,874 - +0.4
476,898 - -0.2
477,017 + +0.2
477,018 - +0.4
477,018 - -1.4
477,018 - +1.2
477,030 - -1.5
477,112 - +1.0
477,238 + H281DRAFT_02321 0.16 -0.1
477,352 + H281DRAFT_02321 0.25 -0.4
477,352 + H281DRAFT_02321 0.25 +0.2
477,352 + H281DRAFT_02321 0.25 -0.7
477,353 - H281DRAFT_02321 0.25 +1.0
477,373 + H281DRAFT_02321 0.27 -0.2
477,373 + H281DRAFT_02321 0.27 +0.1
477,373 + H281DRAFT_02321 0.27 +0.1
477,373 + H281DRAFT_02321 0.27 +0.3
477,373 + H281DRAFT_02321 0.27 +0.3
477,373 + H281DRAFT_02321 0.27 +0.1
477,375 + H281DRAFT_02321 0.27 -0.3
477,375 + H281DRAFT_02321 0.27 +0.5
477,375 + H281DRAFT_02321 0.27 -1.5
477,375 + H281DRAFT_02321 0.27 -0.1
477,375 + H281DRAFT_02321 0.27 +1.0
477,375 + H281DRAFT_02321 0.27 +0.4
477,375 + H281DRAFT_02321 0.27 -0.6
477,375 + H281DRAFT_02321 0.27 -1.3
477,375 + H281DRAFT_02321 0.27 +0.1
477,375 + H281DRAFT_02321 0.27 -0.1
477,375 + H281DRAFT_02321 0.27 -1.6
477,375 + H281DRAFT_02321 0.27 +0.3
477,375 + H281DRAFT_02321 0.27 -0.7
477,375 + H281DRAFT_02321 0.27 -1.1
477,376 - H281DRAFT_02321 0.27 +0.2
477,376 - H281DRAFT_02321 0.27 -0.5
477,376 - H281DRAFT_02321 0.27 -1.1
477,376 - H281DRAFT_02321 0.27 -1.2
477,376 - H281DRAFT_02321 0.27 +0.8
477,376 - H281DRAFT_02321 0.27 +0.4
477,376 - H281DRAFT_02321 0.27 +0.6
477,376 - H281DRAFT_02321 0.27 +0.4
477,376 - H281DRAFT_02321 0.27 +1.1
477,376 - H281DRAFT_02321 0.27 +0.0
477,376 - H281DRAFT_02321 0.27 +0.3
477,377 + H281DRAFT_02321 0.27 -1.6
477,377 + H281DRAFT_02321 0.27 +1.5
477,377 + H281DRAFT_02321 0.27 -0.9
477,377 + H281DRAFT_02321 0.27 -0.0
477,377 + H281DRAFT_02321 0.27 +0.4
477,377 + H281DRAFT_02321 0.27 +0.5
477,378 - H281DRAFT_02321 0.27 -0.2
477,378 - H281DRAFT_02321 0.27 -0.1
477,378 - H281DRAFT_02321 0.27 -0.5
477,378 - H281DRAFT_02321 0.27 -0.8
477,378 - H281DRAFT_02321 0.27 +1.1
477,378 - H281DRAFT_02321 0.27 -0.7
477,580 - H281DRAFT_02321 0.44 +0.5
477,585 + H281DRAFT_02321 0.45 +0.5
477,776 - H281DRAFT_02321 0.60 -1.9
477,866 + H281DRAFT_02321 0.68 +0.5
477,867 - H281DRAFT_02321 0.68 -2.2
477,867 - H281DRAFT_02321 0.68 +0.2
477,883 - H281DRAFT_02321 0.69 -1.3
477,962 - H281DRAFT_02321 0.76 -1.2
477,962 - H281DRAFT_02321 0.76 +0.5
477,962 - H281DRAFT_02321 0.76 -0.1
477,962 - H281DRAFT_02321 0.76 +0.7
477,962 - H281DRAFT_02321 0.76 -0.2
478,277 + -0.5
478,277 + -0.1
478,278 - +0.1
478,278 - -0.2
478,278 - +0.1
478,307 + -0.1
478,385 + -0.2
478,386 - -0.4
478,461 - -0.3
478,522 - H281DRAFT_02322 0.14 +0.3
478,522 - H281DRAFT_02322 0.14 +0.7
478,746 + -0.1
478,747 - -1.2
478,753 + -0.0
478,753 + +0.2
478,753 + +0.8
478,753 + +2.1
478,753 + +0.3
478,754 - -0.1
478,754 - -0.3
478,772 + +0.7
478,772 + +0.7
478,773 - +1.5
478,773 - -0.3
478,777 + -0.1
478,777 + +0.2
478,783 + +0.6
478,783 + -0.6
478,813 + H281DRAFT_02323 0.18 +0.0
478,814 - H281DRAFT_02323 0.18 +0.8
478,834 + H281DRAFT_02323 0.24 +0.2
478,840 + H281DRAFT_02323 0.26 -0.2
478,879 + H281DRAFT_02323 0.37 +0.4
478,879 + H281DRAFT_02323 0.37 +0.8
478,879 + H281DRAFT_02323 0.37 -1.4
478,895 - H281DRAFT_02323 0.41 +0.1
478,909 + H281DRAFT_02323 0.46 +0.8
478,924 + H281DRAFT_02323 0.50 -0.2
478,925 - H281DRAFT_02323 0.50 +0.9
479,002 - H281DRAFT_02323 0.72 +0.3
479,002 - H281DRAFT_02323 0.72 -1.3
479,085 + -0.2
479,086 - +3.3
479,151 + +0.1
479,151 + +0.1
479,151 + -0.0
479,152 - +0.8
479,167 + +0.4
479,178 + -0.6
479,178 + -1.7
479,179 - -0.1

Or see this region's nucleotide sequence