Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02214 and H281DRAFT_02215 overlap by 4 nucleotides H281DRAFT_02215 and H281DRAFT_02216 are separated by 14 nucleotides
H281DRAFT_02214: H281DRAFT_02214 - homoserine O-acetyltransferase, at 356,911 to 358,056
_02214
H281DRAFT_02215: H281DRAFT_02215 - methionine biosynthesis protein MetW, at 358,053 to 358,661
_02215
H281DRAFT_02216: H281DRAFT_02216 - MFS transporter, PAT family, beta-lactamase induction signal transducer AmpG, at 358,676 to 360,070
_02216
Position (kb)
358
359 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 357.097 kb on + strand, within H281DRAFT_02214 at 357.168 kb on + strand, within H281DRAFT_02214 at 357.334 kb on - strand, within H281DRAFT_02214 at 357.400 kb on + strand, within H281DRAFT_02214 at 357.400 kb on + strand, within H281DRAFT_02214 at 357.526 kb on + strand, within H281DRAFT_02214 at 357.526 kb on + strand, within H281DRAFT_02214 at 357.526 kb on + strand, within H281DRAFT_02214 at 357.526 kb on + strand, within H281DRAFT_02214 at 357.527 kb on - strand, within H281DRAFT_02214 at 357.527 kb on - strand, within H281DRAFT_02214 at 357.538 kb on - strand, within H281DRAFT_02214 at 357.592 kb on + strand, within H281DRAFT_02214 at 357.592 kb on + strand, within H281DRAFT_02214 at 357.592 kb on + strand, within H281DRAFT_02214 at 357.593 kb on - strand at 357.593 kb on - strand, within H281DRAFT_02214 at 357.664 kb on + strand, within H281DRAFT_02214 at 357.664 kb on + strand, within H281DRAFT_02214 at 357.665 kb on - strand, within H281DRAFT_02214 at 357.665 kb on - strand, within H281DRAFT_02214 at 357.665 kb on - strand, within H281DRAFT_02214 at 357.665 kb on - strand, within H281DRAFT_02214 at 357.704 kb on - strand, within H281DRAFT_02214 at 357.704 kb on - strand at 357.704 kb on - strand, within H281DRAFT_02214 at 357.737 kb on - strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.754 kb on + strand, within H281DRAFT_02214 at 357.755 kb on - strand, within H281DRAFT_02214 at 357.755 kb on - strand, within H281DRAFT_02214 at 357.755 kb on - strand, within H281DRAFT_02214 at 357.758 kb on + strand, within H281DRAFT_02214 at 357.758 kb on + strand, within H281DRAFT_02214 at 357.758 kb on + strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.759 kb on - strand, within H281DRAFT_02214 at 357.781 kb on + strand, within H281DRAFT_02214 at 357.781 kb on + strand, within H281DRAFT_02214 at 357.782 kb on - strand, within H281DRAFT_02214 at 357.834 kb on + strand, within H281DRAFT_02214 at 357.835 kb on - strand, within H281DRAFT_02214 at 357.835 kb on - strand, within H281DRAFT_02214 at 357.848 kb on - strand, within H281DRAFT_02214 at 357.944 kb on - strand at 358.022 kb on - strand at 358.025 kb on - strand at 358.074 kb on + strand at 358.074 kb on + strand at 358.400 kb on + strand, within H281DRAFT_02215 at 358.400 kb on + strand, within H281DRAFT_02215 at 358.400 kb on + strand, within H281DRAFT_02215 at 358.400 kb on + strand, within H281DRAFT_02215 at 358.400 kb on + strand, within H281DRAFT_02215 at 358.401 kb on - strand, within H281DRAFT_02215 at 358.486 kb on - strand, within H281DRAFT_02215 at 358.628 kb on + strand at 358.628 kb on + strand at 358.629 kb on - strand at 358.629 kb on - strand at 358.641 kb on + strand at 358.642 kb on - strand at 358.659 kb on + strand at 358.660 kb on - strand at 358.708 kb on + strand at 358.708 kb on + strand at 358.709 kb on - strand at 358.750 kb on + strand at 358.751 kb on - strand at 358.783 kb on + strand at 358.783 kb on + strand at 358.815 kb on - strand at 358.815 kb on - strand at 358.815 kb on - strand at 358.815 kb on - strand at 358.893 kb on - strand, within H281DRAFT_02216 at 358.893 kb on - strand, within H281DRAFT_02216 at 358.932 kb on - strand, within H281DRAFT_02216 at 359.183 kb on + strand, within H281DRAFT_02216 at 359.183 kb on + strand, within H281DRAFT_02216 at 359.183 kb on + strand at 359.184 kb on - strand, within H281DRAFT_02216 at 359.199 kb on + strand, within H281DRAFT_02216 at 359.199 kb on + strand, within H281DRAFT_02216 at 359.200 kb on - strand, within H281DRAFT_02216 at 359.200 kb on - strand, within H281DRAFT_02216 at 359.200 kb on - strand, within H281DRAFT_02216 at 359.202 kb on + strand, within H281DRAFT_02216
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 357,097 + H281DRAFT_02214 0.16 -2.8 357,168 + H281DRAFT_02214 0.22 -5.3 357,334 - H281DRAFT_02214 0.37 -4.3 357,400 + H281DRAFT_02214 0.43 -4.0 357,400 + H281DRAFT_02214 0.43 -4.3 357,526 + H281DRAFT_02214 0.54 -1.2 357,526 + H281DRAFT_02214 0.54 -0.5 357,526 + H281DRAFT_02214 0.54 -4.6 357,526 + H281DRAFT_02214 0.54 -4.3 357,527 - H281DRAFT_02214 0.54 -4.4 357,527 - H281DRAFT_02214 0.54 -1.7 357,538 - H281DRAFT_02214 0.55 -4.2 357,592 + H281DRAFT_02214 0.59 -5.2 357,592 + H281DRAFT_02214 0.59 -4.3 357,592 + H281DRAFT_02214 0.59 -5.3 357,593 - +0.1 357,593 - H281DRAFT_02214 0.60 -4.8 357,664 + H281DRAFT_02214 0.66 -2.9 357,664 + H281DRAFT_02214 0.66 -4.0 357,665 - H281DRAFT_02214 0.66 -1.4 357,665 - H281DRAFT_02214 0.66 -4.6 357,665 - H281DRAFT_02214 0.66 -4.1 357,665 - H281DRAFT_02214 0.66 -4.6 357,704 - H281DRAFT_02214 0.69 -5.2 357,704 - +1.1 357,704 - H281DRAFT_02214 0.69 -4.0 357,737 - H281DRAFT_02214 0.72 -4.5 357,754 + H281DRAFT_02214 0.74 -3.6 357,754 + H281DRAFT_02214 0.74 -2.6 357,754 + H281DRAFT_02214 0.74 -5.2 357,754 + H281DRAFT_02214 0.74 -3.9 357,754 + H281DRAFT_02214 0.74 -4.8 357,754 + H281DRAFT_02214 0.74 -3.9 357,755 - H281DRAFT_02214 0.74 -4.7 357,755 - H281DRAFT_02214 0.74 -2.9 357,755 - H281DRAFT_02214 0.74 -3.3 357,758 + H281DRAFT_02214 0.74 -3.1 357,758 + H281DRAFT_02214 0.74 -3.8 357,758 + H281DRAFT_02214 0.74 -3.7 357,759 - H281DRAFT_02214 0.74 -3.8 357,759 - H281DRAFT_02214 0.74 -4.3 357,759 - H281DRAFT_02214 0.74 -5.1 357,759 - H281DRAFT_02214 0.74 -4.9 357,759 - H281DRAFT_02214 0.74 -4.4 357,759 - H281DRAFT_02214 0.74 -2.7 357,781 + H281DRAFT_02214 0.76 -4.1 357,781 + H281DRAFT_02214 0.76 -5.0 357,782 - H281DRAFT_02214 0.76 -0.9 357,834 + H281DRAFT_02214 0.81 -4.0 357,835 - H281DRAFT_02214 0.81 -3.2 357,835 - H281DRAFT_02214 0.81 -3.5 357,848 - H281DRAFT_02214 0.82 -2.3 357,944 - -5.7 358,022 - -5.5 358,025 - +0.1 358,074 + -3.8 358,074 + -1.6 358,400 + H281DRAFT_02215 0.57 -1.7 358,400 + H281DRAFT_02215 0.57 -5.0 358,400 + H281DRAFT_02215 0.57 -1.9 358,400 + H281DRAFT_02215 0.57 -4.7 358,400 + H281DRAFT_02215 0.57 -3.7 358,401 - H281DRAFT_02215 0.57 -2.9 358,486 - H281DRAFT_02215 0.71 -3.5 358,628 + -5.4 358,628 + -2.6 358,629 - -2.3 358,629 - -1.5 358,641 + -4.7 358,642 - -4.7 358,659 + +0.4 358,660 - -0.3 358,708 + -0.6 358,708 + -0.3 358,709 - +0.1 358,750 + -0.1 358,751 - -1.9 358,783 + +1.3 358,783 + -0.7 358,815 - +0.8 358,815 - +0.0 358,815 - +0.1 358,815 - -0.5 358,893 - H281DRAFT_02216 0.16 +0.4 358,893 - H281DRAFT_02216 0.16 -2.7 358,932 - H281DRAFT_02216 0.18 -3.1 359,183 + H281DRAFT_02216 0.36 -0.3 359,183 + H281DRAFT_02216 0.36 -0.6 359,183 + +0.4 359,184 - H281DRAFT_02216 0.36 +0.6 359,199 + H281DRAFT_02216 0.37 +2.1 359,199 + H281DRAFT_02216 0.37 -0.5 359,200 - H281DRAFT_02216 0.38 -0.0 359,200 - H281DRAFT_02216 0.38 -0.4 359,200 - H281DRAFT_02216 0.38 +0.1 359,202 + H281DRAFT_02216 0.38 -0.2
Or see this region's nucleotide sequence