Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02165 and H281DRAFT_02166 are separated by 274 nucleotides H281DRAFT_02166 and H281DRAFT_02167 are separated by 155 nucleotides
H281DRAFT_02165: H281DRAFT_02165 - amidase, at 304,323 to 305,957
_02165
H281DRAFT_02166: H281DRAFT_02166 - High potential iron-sulfur protein, at 306,232 to 306,543
_02166
H281DRAFT_02167: H281DRAFT_02167 - L-2-hydroxyglutarate oxidase LhgO, at 306,699 to 307,805
_02167
Position (kb)
306
307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.334 kb on - strand, within H281DRAFT_02165 at 305.738 kb on + strand, within H281DRAFT_02165 at 305.739 kb on - strand, within H281DRAFT_02165 at 305.812 kb on - strand at 305.812 kb on - strand at 305.939 kb on + strand at 305.939 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.956 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 306.090 kb on + strand at 306.091 kb on - strand at 306.180 kb on + strand at 306.180 kb on + strand at 306.246 kb on + strand at 306.247 kb on - strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.309 kb on - strand, within H281DRAFT_02166 at 306.333 kb on - strand, within H281DRAFT_02166 at 306.368 kb on + strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.413 kb on + strand, within H281DRAFT_02166 at 306.508 kb on - strand, within H281DRAFT_02166 at 306.560 kb on + strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.561 kb on - strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.563 kb on - strand at 306.563 kb on - strand at 306.604 kb on + strand at 306.604 kb on + strand at 306.605 kb on - strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.641 kb on - strand at 306.646 kb on + strand at 306.646 kb on + strand at 306.647 kb on - strand at 306.647 kb on - strand at 306.877 kb on + strand, within H281DRAFT_02167 at 306.877 kb on + strand, within H281DRAFT_02167 at 306.934 kb on + strand, within H281DRAFT_02167 at 306.967 kb on + strand, within H281DRAFT_02167 at 306.967 kb on + strand, within H281DRAFT_02167 at 307.111 kb on + strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.129 kb on + strand, within H281DRAFT_02167 at 307.271 kb on + strand, within H281DRAFT_02167 at 307.271 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.449 kb on - strand, within H281DRAFT_02167
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 305,241 + H281DRAFT_02165 0.56 +0.2 305,241 + H281DRAFT_02165 0.56 -0.0 305,241 + H281DRAFT_02165 0.56 +0.7 305,241 + H281DRAFT_02165 0.56 -0.4 305,241 + H281DRAFT_02165 0.56 +0.2 305,242 - H281DRAFT_02165 0.56 -1.2 305,242 - H281DRAFT_02165 0.56 -0.4 305,242 - H281DRAFT_02165 0.56 -1.3 305,334 - H281DRAFT_02165 0.62 -0.1 305,738 + H281DRAFT_02165 0.87 +0.7 305,739 - H281DRAFT_02165 0.87 -0.2 305,812 - +0.2 305,812 - +0.1 305,939 + +0.3 305,939 + +0.6 305,955 + -0.4 305,955 + +0.6 305,955 + +0.1 305,956 - +0.2 305,990 - -0.8 305,990 - -0.9 305,990 - -1.2 305,990 - -0.5 305,990 - +1.2 305,993 + -0.3 305,993 + -0.6 305,993 + +0.5 305,993 + -1.8 305,994 - -0.9 305,994 - -1.2 305,994 - -0.4 305,994 - +0.1 306,090 + +1.0 306,091 - -0.6 306,180 + +0.4 306,180 + -0.3 306,246 + +0.7 306,247 - +0.3 306,248 + -0.8 306,248 + +0.6 306,248 + +0.1 306,249 - +0.4 306,249 - +2.1 306,249 - -0.7 306,249 - +1.2 306,249 - -0.5 306,309 - H281DRAFT_02166 0.25 +1.1 306,333 - H281DRAFT_02166 0.32 +0.8 306,368 + H281DRAFT_02166 0.44 +0.5 306,369 - H281DRAFT_02166 0.44 +0.3 306,369 - H281DRAFT_02166 0.44 +1.9 306,413 + H281DRAFT_02166 0.58 +0.5 306,508 - H281DRAFT_02166 0.88 +0.4 306,560 + -0.6 306,560 + +0.4 306,560 + +1.4 306,561 - +0.3 306,562 + +1.1 306,562 + +1.0 306,562 + +0.2 306,563 - -0.1 306,563 - +1.4 306,604 + +0.1 306,604 + +0.3 306,605 - +0.5 306,621 + +0.4 306,621 + -0.6 306,621 + +0.1 306,621 + -1.6 306,641 - +0.7 306,646 + +0.3 306,646 + +0.2 306,647 - -0.0 306,647 - -0.3 306,877 + H281DRAFT_02167 0.16 -0.1 306,877 + H281DRAFT_02167 0.16 +0.4 306,934 + H281DRAFT_02167 0.21 +0.6 306,967 + H281DRAFT_02167 0.24 +0.3 306,967 + H281DRAFT_02167 0.24 +0.2 307,111 + H281DRAFT_02167 0.37 +0.6 307,112 - H281DRAFT_02167 0.37 -1.9 307,112 - H281DRAFT_02167 0.37 -0.6 307,112 - H281DRAFT_02167 0.37 +0.8 307,112 - H281DRAFT_02167 0.37 -0.6 307,112 - H281DRAFT_02167 0.37 -0.4 307,129 + H281DRAFT_02167 0.39 -0.5 307,271 + H281DRAFT_02167 0.52 -0.9 307,271 + H281DRAFT_02167 0.52 -0.4 307,448 + H281DRAFT_02167 0.68 -0.2 307,448 + H281DRAFT_02167 0.68 +0.2 307,448 + H281DRAFT_02167 0.68 -1.7 307,448 + H281DRAFT_02167 0.68 -0.2 307,449 - H281DRAFT_02167 0.68 +0.2
Or see this region's nucleotide sequence