Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02000 and H281DRAFT_02001 are separated by 21 nucleotides H281DRAFT_02001 and H281DRAFT_02002 are separated by 150 nucleotides H281DRAFT_02002 and H281DRAFT_02003 overlap by 4 nucleotides H281DRAFT_02003 and H281DRAFT_02004 are separated by 22 nucleotides
H281DRAFT_02000: H281DRAFT_02000 - glycerol-3-phosphate dehydrogenase (NAD(P)+), at 117,851 to 118,849
_02000
H281DRAFT_02001: H281DRAFT_02001 - protein translocase subunit secB, at 118,871 to 119,341
_02001
H281DRAFT_02002: H281DRAFT_02002 - glutaredoxin 3, at 119,492 to 119,752
_02002
H281DRAFT_02003: H281DRAFT_02003 - Rhodanese-related sulfurtransferase, at 119,749 to 120,222
_02003
H281DRAFT_02004: H281DRAFT_02004 - phosphoglycerate mutase, at 120,245 to 121,057
_02004
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 118.295 kb on + strand, within H281DRAFT_02000 at 118.296 kb on - strand, within H281DRAFT_02000 at 118.319 kb on - strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand at 118.543 kb on + strand, within H281DRAFT_02000 at 118.544 kb on - strand, within H281DRAFT_02000 at 118.710 kb on + strand, within H281DRAFT_02000 at 118.849 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 119.360 kb on + strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.364 kb on + strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.370 kb on + strand at 119.403 kb on + strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.453 kb on - strand at 119.453 kb on - strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.734 kb on - strand at 119.759 kb on - strand at 119.853 kb on + strand, within H281DRAFT_02003 at 119.913 kb on + strand, within H281DRAFT_02003 at 119.913 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.156 kb on - strand, within H281DRAFT_02003 at 120.223 kb on + strand at 120.224 kb on - strand at 120.225 kb on + strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.230 kb on + strand at 120.231 kb on - strand at 120.231 kb on - strand at 120.260 kb on + strand at 120.260 kb on + strand at 120.260 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.311 kb on - strand at 120.314 kb on + strand at 120.315 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 118,295 + H281DRAFT_02000 0.44 -3.3 118,296 - H281DRAFT_02000 0.45 -2.7 118,319 - H281DRAFT_02000 0.47 -1.8 118,320 + H281DRAFT_02000 0.47 -1.7 118,320 + H281DRAFT_02000 0.47 -1.5 118,321 - H281DRAFT_02000 0.47 -0.9 118,321 - H281DRAFT_02000 0.47 -2.5 118,321 - -0.5 118,543 + H281DRAFT_02000 0.69 -1.7 118,544 - H281DRAFT_02000 0.69 -1.3 118,710 + H281DRAFT_02000 0.86 -1.3 118,849 + -2.8 118,872 + -1.2 118,872 + -2.2 118,872 + -3.5 118,873 - -2.4 118,873 - -0.3 118,873 - -3.3 118,936 - H281DRAFT_02001 0.14 +0.6 118,936 - H281DRAFT_02001 0.14 -0.3 118,936 - H281DRAFT_02001 0.14 -1.5 118,936 - H281DRAFT_02001 0.14 -0.9 118,936 - H281DRAFT_02001 0.14 -0.9 118,936 - H281DRAFT_02001 0.14 -2.0 118,936 - H281DRAFT_02001 0.14 -1.5 118,936 - H281DRAFT_02001 0.14 -0.5 119,360 + -0.8 119,361 - +0.7 119,361 - -0.3 119,361 - +0.4 119,361 - +0.4 119,361 - +0.3 119,364 + -2.3 119,365 - +0.8 119,365 - +0.2 119,365 - +0.3 119,365 - -2.3 119,365 - +0.7 119,365 - -4.6 119,365 - -0.9 119,365 - +0.2 119,370 + +0.1 119,403 + -1.4 119,404 - -0.7 119,404 - -1.2 119,404 - -0.3 119,404 - +0.0 119,404 - +0.4 119,453 - +0.1 119,453 - -0.4 119,733 + -0.5 119,733 + -0.3 119,733 + -1.7 119,733 + +1.3 119,734 - +0.1 119,759 - +0.3 119,853 + H281DRAFT_02003 0.22 +1.1 119,913 + H281DRAFT_02003 0.35 +0.8 119,913 + H281DRAFT_02003 0.35 +1.3 120,155 + H281DRAFT_02003 0.86 +0.2 120,155 + H281DRAFT_02003 0.86 -0.4 120,155 + H281DRAFT_02003 0.86 -0.2 120,156 - H281DRAFT_02003 0.86 +0.7 120,223 + +0.4 120,224 - +0.1 120,225 + -0.4 120,226 - +1.1 120,226 - -0.3 120,226 - +0.3 120,226 - +0.9 120,230 + +0.5 120,231 - -1.5 120,231 - -0.7 120,260 + +0.5 120,260 + +0.4 120,260 + -1.9 120,310 + +0.2 120,310 + +0.5 120,310 + +0.1 120,310 + +0.6 120,310 + -0.1 120,310 + -1.1 120,311 - -0.3 120,314 + -1.7 120,315 - -0.9
Or see this region's nucleotide sequence