Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01998 and H281DRAFT_01999 overlap by 28 nucleotides H281DRAFT_01999 and H281DRAFT_02000 are separated by 114 nucleotides H281DRAFT_02000 and H281DRAFT_02001 are separated by 21 nucleotides H281DRAFT_02001 and H281DRAFT_02002 are separated by 150 nucleotides H281DRAFT_02002 and H281DRAFT_02003 overlap by 4 nucleotides
H281DRAFT_01998: H281DRAFT_01998 - tRNA (cytidine/uridine-2'-O-)-methyltransferase, at 116,745 to 117,215
_01998
H281DRAFT_01999: H281DRAFT_01999 - O-acetyl-ADP-ribose deacetylase (regulator of RNase III), contains Macro domain, at 117,188 to 117,736
_01999
H281DRAFT_02000: H281DRAFT_02000 - glycerol-3-phosphate dehydrogenase (NAD(P)+), at 117,851 to 118,849
_02000
H281DRAFT_02001: H281DRAFT_02001 - protein translocase subunit secB, at 118,871 to 119,341
_02001
H281DRAFT_02002: H281DRAFT_02002 - glutaredoxin 3, at 119,492 to 119,752
_02002
H281DRAFT_02003: H281DRAFT_02003 - Rhodanese-related sulfurtransferase, at 119,749 to 120,222
_02003
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 116.895 kb on + strand, within H281DRAFT_01998 at 116.896 kb on - strand, within H281DRAFT_01998 at 116.905 kb on - strand, within H281DRAFT_01998 at 116.945 kb on - strand, within H281DRAFT_01998 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.650 kb on - strand, within H281DRAFT_01999 at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.727 kb on + strand at 117.728 kb on - strand at 117.728 kb on - strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.737 kb on - strand at 117.737 kb on - strand at 117.805 kb on + strand at 117.805 kb on + strand at 117.806 kb on - strand at 117.852 kb on + strand at 118.295 kb on + strand, within H281DRAFT_02000 at 118.296 kb on - strand, within H281DRAFT_02000 at 118.319 kb on - strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand at 118.543 kb on + strand, within H281DRAFT_02000 at 118.544 kb on - strand, within H281DRAFT_02000 at 118.710 kb on + strand, within H281DRAFT_02000 at 118.849 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 119.360 kb on + strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.364 kb on + strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.370 kb on + strand at 119.403 kb on + strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.453 kb on - strand at 119.453 kb on - strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.734 kb on - strand at 119.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 116,895 + H281DRAFT_01998 0.32 +0.0 116,896 - H281DRAFT_01998 0.32 -0.2 116,905 - H281DRAFT_01998 0.34 +1.4 116,945 - H281DRAFT_01998 0.42 -0.8 117,508 - H281DRAFT_01999 0.58 +0.4 117,508 - H281DRAFT_01999 0.58 +0.1 117,649 + H281DRAFT_01999 0.84 -0.8 117,649 + H281DRAFT_01999 0.84 -0.1 117,649 + H281DRAFT_01999 0.84 +0.8 117,650 - H281DRAFT_01999 0.84 +0.2 117,725 + +0.4 117,725 + +0.6 117,725 + +0.4 117,725 + +0.1 117,725 + +0.1 117,725 + +0.5 117,726 - -0.7 117,726 - +0.1 117,726 - -0.3 117,726 - +0.4 117,726 - +0.5 117,726 - -0.1 117,726 - +1.4 117,726 - +0.5 117,726 - +0.4 117,726 - -0.5 117,727 + +0.7 117,728 - +0.8 117,728 - +0.5 117,736 + +0.3 117,736 + -1.4 117,736 + -0.3 117,737 - +0.0 117,737 - -0.2 117,805 + +1.7 117,805 + +0.8 117,806 - -0.2 117,852 + +1.0 118,295 + H281DRAFT_02000 0.44 -3.3 118,296 - H281DRAFT_02000 0.45 -2.7 118,319 - H281DRAFT_02000 0.47 -1.8 118,320 + H281DRAFT_02000 0.47 -1.7 118,320 + H281DRAFT_02000 0.47 -1.5 118,321 - H281DRAFT_02000 0.47 -0.9 118,321 - H281DRAFT_02000 0.47 -2.5 118,321 - -0.5 118,543 + H281DRAFT_02000 0.69 -1.7 118,544 - H281DRAFT_02000 0.69 -1.3 118,710 + H281DRAFT_02000 0.86 -1.3 118,849 + -2.8 118,872 + -1.2 118,872 + -2.2 118,872 + -3.5 118,873 - -2.4 118,873 - -0.3 118,873 - -3.3 118,936 - H281DRAFT_02001 0.14 +0.6 118,936 - H281DRAFT_02001 0.14 -0.3 118,936 - H281DRAFT_02001 0.14 -1.5 118,936 - H281DRAFT_02001 0.14 -0.9 118,936 - H281DRAFT_02001 0.14 -0.9 118,936 - H281DRAFT_02001 0.14 -2.0 118,936 - H281DRAFT_02001 0.14 -1.5 118,936 - H281DRAFT_02001 0.14 -0.5 119,360 + -0.8 119,361 - +0.7 119,361 - -0.3 119,361 - +0.4 119,361 - +0.4 119,361 - +0.3 119,364 + -2.3 119,365 - +0.8 119,365 - +0.2 119,365 - +0.3 119,365 - -2.3 119,365 - +0.7 119,365 - -4.6 119,365 - -0.9 119,365 - +0.2 119,370 + +0.1 119,403 + -1.4 119,404 - -0.7 119,404 - -1.2 119,404 - -0.3 119,404 - +0.0 119,404 - +0.4 119,453 - +0.1 119,453 - -0.4 119,733 + -0.5 119,733 + -0.3 119,733 + -1.7 119,733 + +1.3 119,734 - +0.1 119,759 - +0.3
Or see this region's nucleotide sequence