Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01946 and H281DRAFT_01947 are separated by 249 nucleotides H281DRAFT_01947 and H281DRAFT_01948 are separated by 8 nucleotides H281DRAFT_01948 and H281DRAFT_01949 are separated by 550 nucleotides
H281DRAFT_01946: H281DRAFT_01946 - hypothetical protein, at 65,053 to 65,724
_01946
H281DRAFT_01947: H281DRAFT_01947 - hypothetical protein, at 65,974 to 66,240
_01947
H281DRAFT_01948: H281DRAFT_01948 - Tetratricopeptide repeat-containing protein, at 66,249 to 68,051
_01948
H281DRAFT_01949: H281DRAFT_01949 - hypothetical protein, at 68,602 to 69,258
_01949
Position (kb)
66
67
68
69 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 65.263 kb on + strand, within H281DRAFT_01946 at 65.263 kb on + strand, within H281DRAFT_01946 at 65.264 kb on - strand, within H281DRAFT_01946 at 65.264 kb on - strand, within H281DRAFT_01946 at 65.320 kb on + strand, within H281DRAFT_01946 at 65.320 kb on + strand, within H281DRAFT_01946 at 65.320 kb on + strand, within H281DRAFT_01946 at 65.320 kb on + strand, within H281DRAFT_01946 at 65.341 kb on + strand, within H281DRAFT_01946 at 65.341 kb on + strand, within H281DRAFT_01946 at 65.354 kb on + strand, within H281DRAFT_01946 at 65.355 kb on - strand at 65.355 kb on - strand, within H281DRAFT_01946 at 65.462 kb on + strand, within H281DRAFT_01946 at 65.527 kb on - strand, within H281DRAFT_01946 at 65.743 kb on + strand at 65.743 kb on + strand at 65.811 kb on + strand at 65.811 kb on + strand at 65.811 kb on + strand at 65.812 kb on - strand at 65.812 kb on - strand at 65.812 kb on - strand at 65.919 kb on + strand at 65.919 kb on + strand at 65.919 kb on + strand at 65.920 kb on - strand at 65.920 kb on - strand at 65.920 kb on - strand at 65.959 kb on - strand at 65.986 kb on + strand at 65.986 kb on + strand at 65.986 kb on + strand at 65.987 kb on - strand at 65.987 kb on - strand at 66.121 kb on + strand, within H281DRAFT_01947 at 66.121 kb on + strand, within H281DRAFT_01947 at 66.122 kb on - strand, within H281DRAFT_01947 at 66.140 kb on + strand, within H281DRAFT_01947 at 66.140 kb on + strand, within H281DRAFT_01947 at 66.140 kb on + strand, within H281DRAFT_01947 at 66.141 kb on - strand, within H281DRAFT_01947 at 66.212 kb on - strand, within H281DRAFT_01947 at 66.234 kb on + strand at 66.234 kb on + strand at 66.235 kb on - strand at 66.235 kb on - strand at 66.318 kb on + strand at 66.371 kb on + strand at 66.371 kb on + strand at 66.371 kb on + strand at 66.371 kb on + strand at 66.371 kb on + strand at 66.372 kb on - strand at 66.421 kb on - strand at 66.508 kb on - strand, within H281DRAFT_01948 at 66.508 kb on - strand, within H281DRAFT_01948 at 66.554 kb on + strand, within H281DRAFT_01948 at 66.580 kb on - strand, within H281DRAFT_01948 at 66.580 kb on - strand, within H281DRAFT_01948 at 66.580 kb on - strand, within H281DRAFT_01948 at 66.580 kb on - strand, within H281DRAFT_01948 at 66.580 kb on - strand, within H281DRAFT_01948 at 66.627 kb on + strand, within H281DRAFT_01948 at 66.711 kb on + strand, within H281DRAFT_01948 at 66.720 kb on + strand, within H281DRAFT_01948 at 66.857 kb on - strand, within H281DRAFT_01948 at 66.857 kb on - strand, within H281DRAFT_01948 at 66.857 kb on - strand, within H281DRAFT_01948 at 66.860 kb on + strand at 66.860 kb on + strand, within H281DRAFT_01948 at 66.861 kb on - strand, within H281DRAFT_01948 at 66.862 kb on + strand, within H281DRAFT_01948 at 66.862 kb on + strand, within H281DRAFT_01948 at 66.863 kb on - strand, within H281DRAFT_01948 at 66.863 kb on - strand, within H281DRAFT_01948 at 66.868 kb on + strand, within H281DRAFT_01948 at 66.868 kb on + strand, within H281DRAFT_01948 at 66.868 kb on + strand, within H281DRAFT_01948 at 66.869 kb on - strand, within H281DRAFT_01948 at 66.869 kb on - strand, within H281DRAFT_01948 at 66.876 kb on - strand, within H281DRAFT_01948 at 66.953 kb on + strand, within H281DRAFT_01948 at 66.954 kb on - strand, within H281DRAFT_01948 at 67.115 kb on + strand, within H281DRAFT_01948 at 67.116 kb on - strand, within H281DRAFT_01948 at 67.116 kb on - strand, within H281DRAFT_01948 at 67.139 kb on + strand, within H281DRAFT_01948 at 67.139 kb on + strand, within H281DRAFT_01948 at 67.140 kb on - strand, within H281DRAFT_01948 at 67.140 kb on - strand, within H281DRAFT_01948 at 67.192 kb on + strand, within H281DRAFT_01948 at 67.193 kb on - strand, within H281DRAFT_01948 at 67.193 kb on - strand, within H281DRAFT_01948 at 67.193 kb on - strand, within H281DRAFT_01948 at 67.193 kb on - strand, within H281DRAFT_01948 at 67.200 kb on - strand, within H281DRAFT_01948 at 67.210 kb on + strand, within H281DRAFT_01948 at 67.478 kb on + strand, within H281DRAFT_01948 at 67.760 kb on + strand, within H281DRAFT_01948 at 67.760 kb on + strand, within H281DRAFT_01948 at 67.761 kb on - strand, within H281DRAFT_01948 at 67.761 kb on - strand, within H281DRAFT_01948 at 67.780 kb on - strand, within H281DRAFT_01948 at 67.781 kb on + strand, within H281DRAFT_01948 at 67.781 kb on + strand, within H281DRAFT_01948 at 67.879 kb on - strand at 67.879 kb on - strand at 67.966 kb on - strand at 68.035 kb on + strand at 68.036 kb on - strand at 68.036 kb on - strand at 68.050 kb on - strand at 68.079 kb on + strand at 68.079 kb on + strand at 68.079 kb on + strand at 68.079 kb on + strand at 68.079 kb on + strand at 68.079 kb on + strand at 68.080 kb on - strand at 68.080 kb on - strand at 68.080 kb on - strand at 68.080 kb on - strand at 68.106 kb on + strand at 68.106 kb on + strand at 68.106 kb on + strand at 68.107 kb on - strand at 68.107 kb on - strand at 68.107 kb on - strand at 68.110 kb on + strand at 68.111 kb on - strand at 68.111 kb on - strand at 68.123 kb on + strand at 68.124 kb on - strand at 68.136 kb on - strand at 68.216 kb on - strand at 68.285 kb on + strand at 68.285 kb on + strand at 68.285 kb on + strand at 68.286 kb on - strand at 68.286 kb on - strand at 68.328 kb on + strand at 68.328 kb on + strand at 68.329 kb on - strand at 68.331 kb on + strand at 68.351 kb on + strand at 68.351 kb on + strand at 68.351 kb on + strand at 68.352 kb on - strand at 68.352 kb on - strand at 68.353 kb on + strand at 68.354 kb on - strand at 68.403 kb on + strand at 68.403 kb on + strand at 68.403 kb on + strand at 68.403 kb on + strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.404 kb on - strand at 68.474 kb on + strand at 68.475 kb on - strand at 68.574 kb on - strand at 68.574 kb on - strand at 68.597 kb on + strand at 68.597 kb on + strand at 68.598 kb on - strand at 68.598 kb on - strand at 68.598 kb on - strand at 68.598 kb on - strand at 68.601 kb on + strand at 68.601 kb on + strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.602 kb on - strand at 68.697 kb on + strand, within H281DRAFT_01949 at 68.698 kb on - strand, within H281DRAFT_01949 at 68.698 kb on - strand, within H281DRAFT_01949 at 68.717 kb on + strand, within H281DRAFT_01949 at 68.718 kb on - strand at 68.718 kb on - strand, within H281DRAFT_01949 at 68.808 kb on + strand, within H281DRAFT_01949 at 68.808 kb on + strand, within H281DRAFT_01949 at 68.808 kb on + strand, within H281DRAFT_01949 at 68.808 kb on + strand, within H281DRAFT_01949 at 68.809 kb on - strand, within H281DRAFT_01949 at 68.809 kb on - strand at 68.857 kb on + strand, within H281DRAFT_01949 at 68.857 kb on + strand, within H281DRAFT_01949 at 68.857 kb on + strand, within H281DRAFT_01949 at 68.857 kb on + strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.858 kb on - strand, within H281DRAFT_01949 at 68.868 kb on + strand, within H281DRAFT_01949 at 68.869 kb on - strand, within H281DRAFT_01949 at 68.887 kb on + strand, within H281DRAFT_01949 at 68.897 kb on + strand, within H281DRAFT_01949 at 69.022 kb on - strand, within H281DRAFT_01949 at 69.022 kb on - strand, within H281DRAFT_01949
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 65,263 + H281DRAFT_01946 0.31 +0.2 65,263 + H281DRAFT_01946 0.31 -0.0 65,264 - H281DRAFT_01946 0.31 +0.1 65,264 - H281DRAFT_01946 0.31 +1.2 65,320 + H281DRAFT_01946 0.40 +0.1 65,320 + H281DRAFT_01946 0.40 +0.2 65,320 + H281DRAFT_01946 0.40 +0.3 65,320 + H281DRAFT_01946 0.40 -1.4 65,341 + H281DRAFT_01946 0.43 +1.3 65,341 + H281DRAFT_01946 0.43 +0.6 65,354 + H281DRAFT_01946 0.45 +0.1 65,355 - +2.7 65,355 - H281DRAFT_01946 0.45 +1.1 65,462 + H281DRAFT_01946 0.61 -0.2 65,527 - H281DRAFT_01946 0.71 -0.8 65,743 + +1.0 65,743 + +1.2 65,811 + -0.6 65,811 + +0.7 65,811 + +0.3 65,812 - +0.6 65,812 - +0.2 65,812 - -1.5 65,919 + +1.5 65,919 + +0.2 65,919 + +0.3 65,920 - -0.3 65,920 - +0.0 65,920 - -0.3 65,959 - +0.2 65,986 + -0.3 65,986 + -0.2 65,986 + +0.3 65,987 - +0.9 65,987 - -0.4 66,121 + H281DRAFT_01947 0.55 -0.1 66,121 + H281DRAFT_01947 0.55 +0.9 66,122 - H281DRAFT_01947 0.55 -0.7 66,140 + H281DRAFT_01947 0.62 -0.2 66,140 + H281DRAFT_01947 0.62 -0.4 66,140 + H281DRAFT_01947 0.62 -0.6 66,141 - H281DRAFT_01947 0.63 +1.7 66,212 - H281DRAFT_01947 0.89 -0.6 66,234 + +0.3 66,234 + +0.1 66,235 - +0.0 66,235 - -0.0 66,318 + +1.5 66,371 + -0.2 66,371 + +1.0 66,371 + -1.2 66,371 + -0.8 66,371 + -0.1 66,372 - +0.0 66,421 - +0.5 66,508 - H281DRAFT_01948 0.14 +0.1 66,508 - H281DRAFT_01948 0.14 +1.0 66,554 + H281DRAFT_01948 0.17 -0.0 66,580 - H281DRAFT_01948 0.18 -0.6 66,580 - H281DRAFT_01948 0.18 +0.4 66,580 - H281DRAFT_01948 0.18 +1.3 66,580 - H281DRAFT_01948 0.18 -0.4 66,580 - H281DRAFT_01948 0.18 -0.1 66,627 + H281DRAFT_01948 0.21 +0.8 66,711 + H281DRAFT_01948 0.26 -0.8 66,720 + H281DRAFT_01948 0.26 -0.8 66,857 - H281DRAFT_01948 0.34 +0.1 66,857 - H281DRAFT_01948 0.34 +0.2 66,857 - H281DRAFT_01948 0.34 +0.3 66,860 + +1.1 66,860 + H281DRAFT_01948 0.34 -0.4 66,861 - H281DRAFT_01948 0.34 +0.3 66,862 + H281DRAFT_01948 0.34 +0.6 66,862 + H281DRAFT_01948 0.34 +0.1 66,863 - H281DRAFT_01948 0.34 -0.4 66,863 - H281DRAFT_01948 0.34 -0.5 66,868 + H281DRAFT_01948 0.34 +1.0 66,868 + H281DRAFT_01948 0.34 +0.2 66,868 + H281DRAFT_01948 0.34 -0.0 66,869 - H281DRAFT_01948 0.34 +0.6 66,869 - H281DRAFT_01948 0.34 -0.9 66,876 - H281DRAFT_01948 0.35 +0.0 66,953 + H281DRAFT_01948 0.39 +0.7 66,954 - H281DRAFT_01948 0.39 +1.3 67,115 + H281DRAFT_01948 0.48 +1.8 67,116 - H281DRAFT_01948 0.48 +0.3 67,116 - H281DRAFT_01948 0.48 -1.0 67,139 + H281DRAFT_01948 0.49 +0.4 67,139 + H281DRAFT_01948 0.49 +0.4 67,140 - H281DRAFT_01948 0.49 -0.0 67,140 - H281DRAFT_01948 0.49 -0.1 67,192 + H281DRAFT_01948 0.52 +0.7 67,193 - H281DRAFT_01948 0.52 -1.3 67,193 - H281DRAFT_01948 0.52 -0.2 67,193 - H281DRAFT_01948 0.52 -0.3 67,193 - H281DRAFT_01948 0.52 -0.5 67,200 - H281DRAFT_01948 0.53 +0.1 67,210 + H281DRAFT_01948 0.53 -0.5 67,478 + H281DRAFT_01948 0.68 +0.1 67,760 + H281DRAFT_01948 0.84 -1.0 67,760 + H281DRAFT_01948 0.84 +0.5 67,761 - H281DRAFT_01948 0.84 +0.5 67,761 - H281DRAFT_01948 0.84 +0.7 67,780 - H281DRAFT_01948 0.85 -0.6 67,781 + H281DRAFT_01948 0.85 +1.2 67,781 + H281DRAFT_01948 0.85 +0.7 67,879 - -0.9 67,879 - +1.1 67,966 - +0.7 68,035 + +0.2 68,036 - +0.7 68,036 - -0.5 68,050 - -0.4 68,079 + -0.7 68,079 + +0.9 68,079 + +0.8 68,079 + +0.1 68,079 + -0.6 68,079 + +0.1 68,080 - +0.5 68,080 - -0.3 68,080 - -0.7 68,080 - -1.5 68,106 + +0.6 68,106 + +0.1 68,106 + +0.2 68,107 - +1.8 68,107 - +0.1 68,107 - -0.0 68,110 + -0.1 68,111 - -0.0 68,111 - +1.0 68,123 + -0.2 68,124 - +1.7 68,136 - -1.1 68,216 - -0.4 68,285 + +0.8 68,285 + -0.1 68,285 + -0.2 68,286 - -0.2 68,286 - -0.6 68,328 + +0.5 68,328 + +0.8 68,329 - +0.8 68,331 + +0.1 68,351 + +0.6 68,351 + +0.4 68,351 + +0.4 68,352 - +0.3 68,352 - -1.5 68,353 + -0.5 68,354 - -1.1 68,403 + -0.9 68,403 + -1.9 68,403 + -0.0 68,403 + -0.4 68,404 - -0.6 68,404 - +1.1 68,404 - -1.4 68,404 - +0.3 68,404 - +0.0 68,404 - -2.7 68,404 - +1.7 68,404 - +0.3 68,404 - +0.5 68,474 + -0.1 68,475 - +0.4 68,574 - +0.1 68,574 - +0.8 68,597 + +0.1 68,597 + +0.8 68,598 - +0.3 68,598 - +1.2 68,598 - -0.9 68,598 - +0.7 68,601 + +1.1 68,601 + -0.7 68,602 - +0.4 68,602 - +0.1 68,602 - -0.6 68,602 - +1.5 68,602 - -0.7 68,602 - +0.0 68,602 - -3.0 68,697 + H281DRAFT_01949 0.14 +0.0 68,698 - H281DRAFT_01949 0.15 +1.0 68,698 - H281DRAFT_01949 0.15 -0.3 68,717 + H281DRAFT_01949 0.18 -2.7 68,718 - +3.7 68,718 - H281DRAFT_01949 0.18 -0.5 68,808 + H281DRAFT_01949 0.31 +0.1 68,808 + H281DRAFT_01949 0.31 +0.3 68,808 + H281DRAFT_01949 0.31 -1.5 68,808 + H281DRAFT_01949 0.31 +0.2 68,809 - H281DRAFT_01949 0.32 -0.3 68,809 - -0.5 68,857 + H281DRAFT_01949 0.39 -0.8 68,857 + H281DRAFT_01949 0.39 +0.3 68,857 + H281DRAFT_01949 0.39 -1.1 68,857 + H281DRAFT_01949 0.39 +1.1 68,858 - H281DRAFT_01949 0.39 -0.2 68,858 - H281DRAFT_01949 0.39 +0.3 68,858 - H281DRAFT_01949 0.39 -0.7 68,858 - H281DRAFT_01949 0.39 +0.8 68,858 - H281DRAFT_01949 0.39 +0.3 68,858 - H281DRAFT_01949 0.39 -0.1 68,858 - H281DRAFT_01949 0.39 -0.6 68,868 + H281DRAFT_01949 0.40 +0.6 68,869 - H281DRAFT_01949 0.41 -1.5 68,887 + H281DRAFT_01949 0.43 +0.1 68,897 + H281DRAFT_01949 0.45 +0.4 69,022 - H281DRAFT_01949 0.64 +0.3 69,022 - H281DRAFT_01949 0.64 -0.5
Or see this region's nucleotide sequence