Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01940 and H281DRAFT_01941 are separated by 149 nucleotides H281DRAFT_01941 and H281DRAFT_01942 are separated by 114 nucleotides H281DRAFT_01942 and H281DRAFT_01943 are separated by 66 nucleotides H281DRAFT_01943 and H281DRAFT_01944 are separated by 663 nucleotides
H281DRAFT_01940: H281DRAFT_01940 - LSU ribosomal protein L25P, at 56,606 to 57,223
_01940
H281DRAFT_01941: H281DRAFT_01941 - peptidyl-tRNA hydrolase, at 57,373 to 57,975
_01941
H281DRAFT_01942: H281DRAFT_01942 - histidinol-phosphate aminotransferase, at 58,090 to 59,169
_01942
H281DRAFT_01943: H281DRAFT_01943 - Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I), at 59,236 to 59,493
_01943
H281DRAFT_01944: H281DRAFT_01944 - Rad3-related DNA helicase, at 60,157 to 62,745
_01944
Position (kb) 58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 57.322 kb on + strand at 57.928 kb on - strand at 57.973 kb on + strand at 57.973 kb on + strand at 57.974 kb on - strand at 57.974 kb on - strand at 58.078 kb on + strand at 58.132 kb on + strand at 58.133 kb on - strand at 58.133 kb on - strand at 58.401 kb on + strand, within H281DRAFT_01942 at 58.401 kb on + strand, within H281DRAFT_01942 at 58.401 kb on + strand, within H281DRAFT_01942 at 58.401 kb on + strand, within H281DRAFT_01942 at 58.402 kb on - strand, within H281DRAFT_01942 at 58.402 kb on - strand, within H281DRAFT_01942 at 58.402 kb on - strand, within H281DRAFT_01942 at 58.402 kb on - strand, within H281DRAFT_01942 at 58.402 kb on - strand, within H281DRAFT_01942 at 58.429 kb on + strand, within H281DRAFT_01942 at 58.429 kb on + strand, within H281DRAFT_01942 at 58.429 kb on + strand, within H281DRAFT_01942 at 58.430 kb on - strand, within H281DRAFT_01942 at 58.430 kb on - strand, within H281DRAFT_01942 at 58.438 kb on + strand, within H281DRAFT_01942 at 58.447 kb on + strand, within H281DRAFT_01942 at 58.447 kb on + strand, within H281DRAFT_01942 at 58.447 kb on + strand, within H281DRAFT_01942 at 58.519 kb on + strand, within H281DRAFT_01942 at 58.520 kb on - strand at 58.608 kb on - strand, within H281DRAFT_01942 at 58.648 kb on + strand, within H281DRAFT_01942 at 58.648 kb on + strand, within H281DRAFT_01942 at 58.648 kb on + strand, within H281DRAFT_01942 at 58.649 kb on - strand, within H281DRAFT_01942 at 58.802 kb on + strand, within H281DRAFT_01942 at 58.803 kb on - strand, within H281DRAFT_01942 at 58.803 kb on - strand, within H281DRAFT_01942 at 58.865 kb on - strand, within H281DRAFT_01942 at 58.904 kb on + strand, within H281DRAFT_01942 at 58.904 kb on + strand, within H281DRAFT_01942 at 58.904 kb on + strand, within H281DRAFT_01942 at 58.905 kb on - strand, within H281DRAFT_01942 at 58.938 kb on - strand, within H281DRAFT_01942 at 58.946 kb on + strand, within H281DRAFT_01942 at 58.946 kb on + strand, within H281DRAFT_01942 at 58.974 kb on + strand, within H281DRAFT_01942 at 59.002 kb on + strand, within H281DRAFT_01942 at 59.002 kb on + strand, within H281DRAFT_01942 at 59.003 kb on - strand, within H281DRAFT_01942 at 59.003 kb on - strand, within H281DRAFT_01942 at 59.022 kb on - strand, within H281DRAFT_01942 at 59.158 kb on + strand at 59.158 kb on + strand at 59.158 kb on + strand at 59.159 kb on - strand at 59.159 kb on - strand at 59.159 kb on - strand at 59.159 kb on - strand at 59.267 kb on + strand, within H281DRAFT_01943 at 59.268 kb on - strand, within H281DRAFT_01943 at 59.524 kb on - strand at 59.526 kb on - strand at 59.526 kb on - strand at 59.526 kb on - strand at 59.671 kb on + strand at 59.671 kb on + strand at 59.671 kb on + strand at 59.672 kb on - strand at 59.672 kb on - strand at 59.687 kb on + strand at 59.687 kb on + strand at 59.687 kb on + strand at 59.687 kb on + strand at 59.688 kb on - strand at 59.688 kb on - strand at 59.688 kb on - strand at 59.737 kb on + strand at 59.737 kb on + strand at 59.737 kb on + strand at 59.775 kb on - strand at 59.969 kb on + strand at 59.969 kb on + strand at 59.993 kb on + strand at 59.993 kb on + strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 59.994 kb on - strand at 60.014 kb on - strand at 60.052 kb on + strand at 60.052 kb on + strand at 60.052 kb on + strand at 60.053 kb on - strand at 60.053 kb on - strand at 60.117 kb on - strand at 60.117 kb on - strand at 60.136 kb on + strand at 60.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 57,322 + +0.3 57,928 - -2.9 57,973 + +1.3 57,973 + +0.4 57,974 - -0.1 57,974 - -1.6 58,078 + -0.6 58,132 + +0.1 58,133 - +1.0 58,133 - +0.2 58,401 + H281DRAFT_01942 0.29 -1.6 58,401 + H281DRAFT_01942 0.29 -1.6 58,401 + H281DRAFT_01942 0.29 +0.2 58,401 + H281DRAFT_01942 0.29 +0.8 58,402 - H281DRAFT_01942 0.29 +0.3 58,402 - H281DRAFT_01942 0.29 +0.4 58,402 - H281DRAFT_01942 0.29 +2.9 58,402 - H281DRAFT_01942 0.29 +1.1 58,402 - H281DRAFT_01942 0.29 +0.2 58,429 + H281DRAFT_01942 0.31 -3.1 58,429 + H281DRAFT_01942 0.31 -0.6 58,429 + H281DRAFT_01942 0.31 +0.5 58,430 - H281DRAFT_01942 0.31 +0.4 58,430 - H281DRAFT_01942 0.31 +0.9 58,438 + H281DRAFT_01942 0.32 -0.1 58,447 + H281DRAFT_01942 0.33 -0.1 58,447 + H281DRAFT_01942 0.33 +0.1 58,447 + H281DRAFT_01942 0.33 +0.3 58,519 + H281DRAFT_01942 0.40 -0.0 58,520 - -0.5 58,608 - H281DRAFT_01942 0.48 +0.1 58,648 + H281DRAFT_01942 0.52 +1.2 58,648 + H281DRAFT_01942 0.52 +0.1 58,648 + H281DRAFT_01942 0.52 +0.9 58,649 - H281DRAFT_01942 0.52 -0.3 58,802 + H281DRAFT_01942 0.66 +1.1 58,803 - H281DRAFT_01942 0.66 +0.1 58,803 - H281DRAFT_01942 0.66 +0.2 58,865 - H281DRAFT_01942 0.72 +0.4 58,904 + H281DRAFT_01942 0.75 +0.9 58,904 + H281DRAFT_01942 0.75 -0.3 58,904 + H281DRAFT_01942 0.75 -0.5 58,905 - H281DRAFT_01942 0.75 +0.5 58,938 - H281DRAFT_01942 0.79 -0.0 58,946 + H281DRAFT_01942 0.79 +0.1 58,946 + H281DRAFT_01942 0.79 -0.1 58,974 + H281DRAFT_01942 0.82 +0.4 59,002 + H281DRAFT_01942 0.84 +0.8 59,002 + H281DRAFT_01942 0.84 -0.5 59,003 - H281DRAFT_01942 0.85 +0.5 59,003 - H281DRAFT_01942 0.85 +1.3 59,022 - H281DRAFT_01942 0.86 -0.0 59,158 + +0.1 59,158 + +0.4 59,158 + +0.4 59,159 - +2.1 59,159 - +0.7 59,159 - -0.7 59,159 - -0.2 59,267 + H281DRAFT_01943 0.12 -0.1 59,268 - H281DRAFT_01943 0.12 -0.1 59,524 - +1.1 59,526 - -0.0 59,526 - -0.2 59,526 - -2.0 59,671 + +0.3 59,671 + -0.1 59,671 + +0.1 59,672 - +0.3 59,672 - +0.3 59,687 + +0.1 59,687 + -0.3 59,687 + +1.0 59,687 + +0.1 59,688 - +0.1 59,688 - +1.0 59,688 - +0.8 59,737 + +0.8 59,737 + +0.7 59,737 + -1.8 59,775 - +0.1 59,969 + +0.8 59,969 + +0.3 59,993 + -0.8 59,993 + -1.5 59,994 - +0.4 59,994 - -0.4 59,994 - +0.5 59,994 - -1.7 59,994 - -0.3 59,994 - +1.4 59,994 - -0.9 59,994 - +0.6 59,994 - -0.3 59,994 - +0.1 60,014 - +0.2 60,052 + +0.3 60,052 + -0.4 60,052 + +1.1 60,053 - +0.7 60,053 - +0.8 60,117 - +0.5 60,117 - +0.4 60,136 + +0.3 60,137 - -1.0
Or see this region's nucleotide sequence