Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01109 and H281DRAFT_01110 are separated by 34 nucleotides H281DRAFT_01110 and H281DRAFT_01111 are separated by 285 nucleotides H281DRAFT_01111 and H281DRAFT_01112 are separated by 109 nucleotides
H281DRAFT_01109: H281DRAFT_01109 - hypothetical protein, at 1,249,579 to 1,249,845
_01109
H281DRAFT_01110: H281DRAFT_01110 - Protein of unknown function (DUF2934), at 1,249,880 to 1,250,179
_01110
H281DRAFT_01111: H281DRAFT_01111 - transcriptional regulator, DeoR family, at 1,250,465 to 1,251,190
_01111
H281DRAFT_01112: H281DRAFT_01112 - deoxynucleoside transporter, permease component 2 (from data) , at 1,251,300 to 1,252,394
_01112
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1249.598 kb on + strand at 1249.833 kb on - strand at 1249.864 kb on + strand at 1250.150 kb on + strand at 1250.151 kb on - strand at 1250.151 kb on - strand at 1250.184 kb on - strand at 1250.427 kb on + strand at 1250.829 kb on - strand, within H281DRAFT_01111 at 1250.898 kb on - strand, within H281DRAFT_01111 at 1251.200 kb on + strand at 1251.211 kb on - strand at 1251.263 kb on + strand at 1251.344 kb on + strand at 1251.433 kb on - strand, within H281DRAFT_01112 at 1251.674 kb on - strand, within H281DRAFT_01112 at 1251.788 kb on - strand, within H281DRAFT_01112 at 1252.045 kb on + strand, within H281DRAFT_01112
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 1,249,598 + +1.6 1,249,833 - +2.1 1,249,864 + -0.4 1,250,150 + -0.8 1,250,151 - +0.0 1,250,151 - +0.8 1,250,184 - -0.3 1,250,427 + +0.5 1,250,829 - H281DRAFT_01111 0.50 +0.3 1,250,898 - H281DRAFT_01111 0.60 +0.3 1,251,200 + +0.9 1,251,211 - +1.2 1,251,263 + +0.6 1,251,344 + -1.7 1,251,433 - H281DRAFT_01112 0.12 +0.8 1,251,674 - H281DRAFT_01112 0.34 -0.2 1,251,788 - H281DRAFT_01112 0.45 -1.2 1,252,045 + H281DRAFT_01112 0.68 -2.8
Or see this region's nucleotide sequence