Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00487

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00486 and H281DRAFT_00487 are separated by 103 nucleotidesH281DRAFT_00487 and H281DRAFT_00488 overlap by 52 nucleotides H281DRAFT_00486: H281DRAFT_00486 - lysyl-tRNA synthetase, class II, at 543,065 to 544,675 _00486 H281DRAFT_00487: H281DRAFT_00487 - bacterial peptide chain release factor 2 (bRF-2), at 544,779 to 546,098 _00487 H281DRAFT_00488: H281DRAFT_00488 - exonuclease RecJ, at 546,047 to 547,753 _00488 Position (kb) 544 545 546 547Strain fitness (log2 ratio) -2 -1 0 1at 544.633 kb on - strandat 545.897 kb on - strand, within H281DRAFT_00487at 545.919 kb on - strand, within H281DRAFT_00487at 546.048 kb on + strandat 546.048 kb on + strandat 546.118 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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544,633 - +0.0
545,897 - H281DRAFT_00487 0.85 +0.4
545,919 - H281DRAFT_00487 0.86 +1.1
546,048 + -1.0
546,048 + -2.6
546,118 - -1.1

Or see this region's nucleotide sequence