Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00200 and H281DRAFT_00201 are separated by 199 nucleotides H281DRAFT_00201 and H281DRAFT_00202 are separated by 158 nucleotides H281DRAFT_00202 and H281DRAFT_00203 are separated by 12 nucleotides
H281DRAFT_00200: H281DRAFT_00200 - HemY protein, at 218,070 to 219,260
_00200
H281DRAFT_00201: H281DRAFT_00201 - hypothetical protein, at 219,460 to 219,777
_00201
H281DRAFT_00202: H281DRAFT_00202 - RNA polymerase sigma factor, sigma-70 family, at 219,936 to 220,517
_00202
H281DRAFT_00203: H281DRAFT_00203 - hypothetical protein, at 220,530 to 220,838
_00203
Position (kb) 219
220 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 218.475 kb on + strand at 218.867 kb on - strand, within H281DRAFT_00200 at 219.436 kb on + strand at 219.461 kb on + strand at 219.461 kb on + strand at 219.495 kb on + strand, within H281DRAFT_00201 at 219.496 kb on - strand, within H281DRAFT_00201 at 219.768 kb on - strand at 219.811 kb on + strand at 220.069 kb on + strand, within H281DRAFT_00202 at 220.069 kb on + strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.296 kb on + strand, within H281DRAFT_00202 at 220.436 kb on + strand at 220.437 kb on - strand, within H281DRAFT_00202 at 220.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 218,475 + +1.3 218,867 - H281DRAFT_00200 0.67 -0.1 219,436 + +1.1 219,461 + -1.2 219,461 + -0.1 219,495 + H281DRAFT_00201 0.11 +0.5 219,496 - H281DRAFT_00201 0.11 +2.4 219,768 - -0.3 219,811 + -1.5 220,069 + H281DRAFT_00202 0.23 -2.7 220,069 + H281DRAFT_00202 0.23 +1.4 220,070 - H281DRAFT_00202 0.23 +0.6 220,070 - H281DRAFT_00202 0.23 +1.1 220,070 - H281DRAFT_00202 0.23 -0.3 220,296 + H281DRAFT_00202 0.62 -0.1 220,436 + +3.2 220,437 - H281DRAFT_00202 0.86 +1.4 220,515 + -0.6
Or see this region's nucleotide sequence