Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00153 and H281DRAFT_00154 are separated by 28 nucleotides H281DRAFT_00154 and H281DRAFT_00155 are separated by 151 nucleotides
H281DRAFT_00153: H281DRAFT_00153 - autotransporter secretion outer membrane protein TamA, at 157,219 to 158,979
_00153
H281DRAFT_00154: H281DRAFT_00154 - autotransporter secretion inner membrane protein TamB, at 159,008 to 163,381
_00154
H281DRAFT_00155: H281DRAFT_00155 - methionyl-tRNA synthetase, at 163,533 to 165,671
_00155
Position (kb) 159
160
161
162
163
164 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 158.039 kb on + strand, within H281DRAFT_00153 at 158.047 kb on + strand, within H281DRAFT_00153 at 158.053 kb on + strand, within H281DRAFT_00153 at 158.053 kb on + strand, within H281DRAFT_00153 at 158.053 kb on + strand, within H281DRAFT_00153 at 158.054 kb on - strand, within H281DRAFT_00153 at 158.123 kb on - strand, within H281DRAFT_00153 at 158.123 kb on - strand, within H281DRAFT_00153 at 158.123 kb on - strand, within H281DRAFT_00153 at 158.130 kb on + strand, within H281DRAFT_00153 at 158.130 kb on + strand, within H281DRAFT_00153 at 158.131 kb on - strand, within H281DRAFT_00153 at 158.238 kb on + strand, within H281DRAFT_00153 at 158.239 kb on - strand, within H281DRAFT_00153 at 158.255 kb on - strand, within H281DRAFT_00153 at 158.283 kb on + strand, within H281DRAFT_00153 at 158.288 kb on - strand, within H281DRAFT_00153 at 158.338 kb on + strand, within H281DRAFT_00153 at 158.344 kb on + strand, within H281DRAFT_00153 at 158.344 kb on + strand, within H281DRAFT_00153 at 158.344 kb on + strand, within H281DRAFT_00153 at 158.344 kb on + strand, within H281DRAFT_00153 at 158.344 kb on + strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.345 kb on - strand, within H281DRAFT_00153 at 158.359 kb on + strand, within H281DRAFT_00153 at 158.360 kb on - strand, within H281DRAFT_00153 at 158.504 kb on + strand, within H281DRAFT_00153 at 158.505 kb on - strand, within H281DRAFT_00153 at 158.615 kb on + strand, within H281DRAFT_00153 at 158.616 kb on - strand, within H281DRAFT_00153 at 158.698 kb on + strand, within H281DRAFT_00153 at 158.712 kb on + strand, within H281DRAFT_00153 at 158.713 kb on - strand, within H281DRAFT_00153 at 158.746 kb on + strand, within H281DRAFT_00153 at 158.746 kb on + strand, within H281DRAFT_00153 at 158.746 kb on + strand, within H281DRAFT_00153 at 158.746 kb on + strand, within H281DRAFT_00153 at 158.746 kb on + strand, within H281DRAFT_00153 at 158.747 kb on - strand, within H281DRAFT_00153 at 158.747 kb on - strand, within H281DRAFT_00153 at 158.747 kb on - strand, within H281DRAFT_00153 at 158.747 kb on - strand, within H281DRAFT_00153 at 158.771 kb on - strand, within H281DRAFT_00153 at 158.771 kb on - strand, within H281DRAFT_00153 at 158.771 kb on - strand, within H281DRAFT_00153 at 158.771 kb on - strand, within H281DRAFT_00153 at 158.771 kb on - strand, within H281DRAFT_00153 at 158.924 kb on - strand at 158.994 kb on + strand at 159.239 kb on + strand at 159.239 kb on + strand at 159.239 kb on + strand at 159.239 kb on + strand at 159.382 kb on + strand at 159.383 kb on - strand at 159.383 kb on - strand at 159.434 kb on + strand at 159.448 kb on + strand, within H281DRAFT_00154 at 159.735 kb on + strand, within H281DRAFT_00154 at 159.735 kb on + strand at 159.735 kb on + strand, within H281DRAFT_00154 at 159.736 kb on - strand, within H281DRAFT_00154 at 159.741 kb on - strand, within H281DRAFT_00154 at 159.769 kb on - strand, within H281DRAFT_00154 at 160.183 kb on + strand, within H281DRAFT_00154 at 160.561 kb on + strand, within H281DRAFT_00154 at 160.561 kb on + strand, within H281DRAFT_00154 at 160.562 kb on - strand, within H281DRAFT_00154 at 160.562 kb on - strand, within H281DRAFT_00154 at 160.625 kb on + strand, within H281DRAFT_00154 at 160.626 kb on - strand, within H281DRAFT_00154 at 161.255 kb on + strand, within H281DRAFT_00154 at 161.413 kb on - strand, within H281DRAFT_00154 at 161.803 kb on - strand, within H281DRAFT_00154 at 162.140 kb on + strand, within H281DRAFT_00154 at 162.141 kb on - strand, within H281DRAFT_00154 at 162.199 kb on + strand, within H281DRAFT_00154 at 162.279 kb on + strand, within H281DRAFT_00154 at 162.279 kb on + strand, within H281DRAFT_00154 at 162.279 kb on + strand, within H281DRAFT_00154 at 162.445 kb on + strand, within H281DRAFT_00154 at 162.445 kb on + strand, within H281DRAFT_00154 at 162.446 kb on - strand, within H281DRAFT_00154 at 162.446 kb on - strand, within H281DRAFT_00154 at 162.647 kb on - strand, within H281DRAFT_00154 at 162.821 kb on + strand, within H281DRAFT_00154 at 162.821 kb on + strand, within H281DRAFT_00154 at 162.821 kb on + strand, within H281DRAFT_00154 at 162.839 kb on - strand, within H281DRAFT_00154 at 163.239 kb on - strand at 163.239 kb on - strand at 163.298 kb on + strand at 163.298 kb on + strand at 163.347 kb on + strand at 163.348 kb on - strand at 163.349 kb on + strand at 163.349 kb on + strand at 163.349 kb on + strand at 163.350 kb on - strand at 163.464 kb on + strand at 163.492 kb on + strand at 163.515 kb on + strand at 163.516 kb on - strand at 163.516 kb on - strand at 163.519 kb on + strand at 163.520 kb on - strand at 163.520 kb on - strand at 163.520 kb on - strand at 163.534 kb on + strand at 163.534 kb on + strand at 163.771 kb on - strand, within H281DRAFT_00155
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 158,039 + H281DRAFT_00153 0.47 -0.9 158,047 + H281DRAFT_00153 0.47 +0.0 158,053 + H281DRAFT_00153 0.47 +0.6 158,053 + H281DRAFT_00153 0.47 +0.5 158,053 + H281DRAFT_00153 0.47 +0.7 158,054 - H281DRAFT_00153 0.47 +1.2 158,123 - H281DRAFT_00153 0.51 +0.3 158,123 - H281DRAFT_00153 0.51 +0.7 158,123 - H281DRAFT_00153 0.51 -0.3 158,130 + H281DRAFT_00153 0.52 -0.4 158,130 + H281DRAFT_00153 0.52 +1.7 158,131 - H281DRAFT_00153 0.52 +0.7 158,238 + H281DRAFT_00153 0.58 +0.6 158,239 - H281DRAFT_00153 0.58 +0.5 158,255 - H281DRAFT_00153 0.59 -0.4 158,283 + H281DRAFT_00153 0.60 +0.6 158,288 - H281DRAFT_00153 0.61 -0.8 158,338 + H281DRAFT_00153 0.64 +0.3 158,344 + H281DRAFT_00153 0.64 +0.6 158,344 + H281DRAFT_00153 0.64 +1.4 158,344 + H281DRAFT_00153 0.64 +0.8 158,344 + H281DRAFT_00153 0.64 +0.2 158,344 + H281DRAFT_00153 0.64 +1.3 158,345 - H281DRAFT_00153 0.64 -0.6 158,345 - H281DRAFT_00153 0.64 +0.8 158,345 - H281DRAFT_00153 0.64 -0.5 158,345 - H281DRAFT_00153 0.64 -0.7 158,345 - H281DRAFT_00153 0.64 -0.6 158,345 - H281DRAFT_00153 0.64 -0.6 158,359 + H281DRAFT_00153 0.65 +0.4 158,360 - H281DRAFT_00153 0.65 -0.2 158,504 + H281DRAFT_00153 0.73 +1.2 158,505 - H281DRAFT_00153 0.73 +0.4 158,615 + H281DRAFT_00153 0.79 +1.4 158,616 - H281DRAFT_00153 0.79 -0.3 158,698 + H281DRAFT_00153 0.84 +2.8 158,712 + H281DRAFT_00153 0.85 +1.0 158,713 - H281DRAFT_00153 0.85 +0.3 158,746 + H281DRAFT_00153 0.87 +0.8 158,746 + H281DRAFT_00153 0.87 +0.6 158,746 + H281DRAFT_00153 0.87 +1.0 158,746 + H281DRAFT_00153 0.87 +1.6 158,746 + H281DRAFT_00153 0.87 +1.0 158,747 - H281DRAFT_00153 0.87 +0.0 158,747 - H281DRAFT_00153 0.87 -0.3 158,747 - H281DRAFT_00153 0.87 -0.0 158,747 - H281DRAFT_00153 0.87 -0.3 158,771 - H281DRAFT_00153 0.88 -1.0 158,771 - H281DRAFT_00153 0.88 -0.2 158,771 - H281DRAFT_00153 0.88 +0.7 158,771 - H281DRAFT_00153 0.88 +1.7 158,771 - H281DRAFT_00153 0.88 +0.0 158,924 - -0.7 158,994 + +1.3 159,239 + +1.0 159,239 + -0.5 159,239 + -0.0 159,239 + +0.2 159,382 + +0.4 159,383 - -0.3 159,383 - +0.0 159,434 + -0.5 159,448 + H281DRAFT_00154 0.10 -0.8 159,735 + H281DRAFT_00154 0.17 +0.0 159,735 + +1.4 159,735 + H281DRAFT_00154 0.17 -0.9 159,736 - H281DRAFT_00154 0.17 -0.5 159,741 - H281DRAFT_00154 0.17 -0.7 159,769 - H281DRAFT_00154 0.17 -0.8 160,183 + H281DRAFT_00154 0.27 -0.1 160,561 + H281DRAFT_00154 0.36 +0.4 160,561 + H281DRAFT_00154 0.36 -1.9 160,562 - H281DRAFT_00154 0.36 -4.0 160,562 - H281DRAFT_00154 0.36 +0.6 160,625 + H281DRAFT_00154 0.37 -0.7 160,626 - H281DRAFT_00154 0.37 +0.5 161,255 + H281DRAFT_00154 0.51 -0.2 161,413 - H281DRAFT_00154 0.55 -0.8 161,803 - H281DRAFT_00154 0.64 -0.0 162,140 + H281DRAFT_00154 0.72 -0.1 162,141 - H281DRAFT_00154 0.72 -0.0 162,199 + H281DRAFT_00154 0.73 +0.7 162,279 + H281DRAFT_00154 0.75 +0.3 162,279 + H281DRAFT_00154 0.75 +0.8 162,279 + H281DRAFT_00154 0.75 -0.6 162,445 + H281DRAFT_00154 0.79 -0.4 162,445 + H281DRAFT_00154 0.79 -0.3 162,446 - H281DRAFT_00154 0.79 -2.2 162,446 - H281DRAFT_00154 0.79 +2.4 162,647 - H281DRAFT_00154 0.83 -0.1 162,821 + H281DRAFT_00154 0.87 -0.1 162,821 + H281DRAFT_00154 0.87 -0.2 162,821 + H281DRAFT_00154 0.87 -0.7 162,839 - H281DRAFT_00154 0.88 -0.4 163,239 - -1.1 163,239 - -1.0 163,298 + -3.2 163,298 + +0.8 163,347 + +0.4 163,348 - -0.8 163,349 + +0.5 163,349 + -0.0 163,349 + -1.3 163,350 - +0.2 163,464 + -0.3 163,492 + +0.3 163,515 + -0.3 163,516 - +0.3 163,516 - +0.5 163,519 + +1.0 163,520 - +2.0 163,520 - +0.3 163,520 - +0.1 163,534 + +0.5 163,534 + -0.6 163,771 - H281DRAFT_00155 0.11 -4.2
Or see this region's nucleotide sequence