Experiment: ICP1_phage_23.5_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_18865 and CSW01_18870 are separated by 4 nucleotides CSW01_18870 and CSW01_18875 are separated by 137 nucleotides CSW01_18875 and CSW01_18880 are separated by 206 nucleotides CSW01_18880 and CSW01_18885 are separated by 166 nucleotides
CSW01_18865: CSW01_18865 - spermidine acetyltransferase, at 897,393 to 897,914
_18865
CSW01_18870: CSW01_18870 - transcriptional regulator, at 897,919 to 898,164
_18870
CSW01_18875: CSW01_18875 - SM-20 protein, at 898,302 to 898,919
_18875
CSW01_18880: CSW01_18880 - hypothetical protein, at 899,126 to 899,446
_18880
CSW01_18885: CSW01_18885 - helix-turn-helix transcriptional regulator, at 899,613 to 900,287
_18885
Position (kb) 898
899 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 897.302 kb on + strand at 897.310 kb on - strand at 897.310 kb on - strand at 897.310 kb on - strand at 897.492 kb on - strand, within CSW01_18865 at 897.635 kb on - strand, within CSW01_18865 at 897.675 kb on + strand, within CSW01_18865 at 897.675 kb on + strand, within CSW01_18865 at 897.773 kb on + strand, within CSW01_18865 at 897.773 kb on + strand, within CSW01_18865 at 897.781 kb on - strand, within CSW01_18865 at 897.781 kb on - strand, within CSW01_18865 at 897.781 kb on - strand, within CSW01_18865 at 897.781 kb on - strand, within CSW01_18865 at 897.882 kb on - strand at 897.924 kb on - strand at 897.962 kb on - strand, within CSW01_18870 at 898.003 kb on + strand, within CSW01_18870 at 898.013 kb on - strand, within CSW01_18870 at 898.013 kb on - strand, within CSW01_18870 at 898.053 kb on - strand, within CSW01_18870 at 898.097 kb on - strand, within CSW01_18870 at 898.181 kb on - strand at 898.186 kb on - strand at 898.186 kb on - strand at 898.206 kb on + strand at 898.206 kb on + strand at 898.251 kb on + strand at 898.270 kb on - strand at 898.317 kb on - strand at 898.319 kb on + strand at 898.360 kb on + strand at 898.522 kb on + strand, within CSW01_18875 at 898.569 kb on + strand, within CSW01_18875 at 898.577 kb on + strand, within CSW01_18875 at 898.592 kb on - strand, within CSW01_18875 at 898.624 kb on + strand, within CSW01_18875 at 898.658 kb on + strand, within CSW01_18875 at 898.663 kb on + strand, within CSW01_18875 at 898.671 kb on - strand, within CSW01_18875 at 898.692 kb on + strand, within CSW01_18875 at 898.700 kb on - strand, within CSW01_18875 at 898.700 kb on - strand, within CSW01_18875 at 898.817 kb on + strand, within CSW01_18875 at 898.817 kb on + strand, within CSW01_18875 at 898.824 kb on + strand, within CSW01_18875 at 898.825 kb on - strand, within CSW01_18875 at 898.825 kb on - strand, within CSW01_18875 at 898.825 kb on - strand, within CSW01_18875 at 898.840 kb on - strand, within CSW01_18875 at 899.146 kb on - strand at 899.263 kb on + strand, within CSW01_18880 at 899.263 kb on + strand, within CSW01_18880 at 899.271 kb on - strand, within CSW01_18880 at 899.279 kb on - strand, within CSW01_18880 at 899.324 kb on - strand, within CSW01_18880 at 899.400 kb on - strand, within CSW01_18880 at 899.424 kb on + strand at 899.459 kb on - strand at 899.460 kb on - strand at 899.460 kb on - strand at 899.465 kb on + strand at 899.528 kb on + strand at 899.532 kb on + strand at 899.542 kb on + strand at 899.542 kb on + strand at 899.542 kb on + strand at 899.542 kb on + strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.598 kb on + strand at 899.598 kb on + strand at 899.598 kb on + strand at 899.606 kb on - strand at 899.606 kb on - strand at 899.650 kb on + strand at 899.655 kb on + strand at 899.655 kb on + strand at 899.720 kb on - strand, within CSW01_18885 at 899.739 kb on + strand, within CSW01_18885 at 899.832 kb on + strand, within CSW01_18885 at 899.840 kb on - strand, within CSW01_18885 at 899.845 kb on - strand, within CSW01_18885 at 899.854 kb on + strand, within CSW01_18885 at 899.882 kb on + strand, within CSW01_18885 at 899.890 kb on - strand, within CSW01_18885 at 899.890 kb on - strand, within CSW01_18885
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep1 remove 897,302 + +0.9 897,310 - +0.7 897,310 - -3.2 897,310 - -1.1 897,492 - CSW01_18865 0.19 +1.1 897,635 - CSW01_18865 0.46 -0.5 897,675 + CSW01_18865 0.54 -0.2 897,675 + CSW01_18865 0.54 +1.1 897,773 + CSW01_18865 0.73 -0.2 897,773 + CSW01_18865 0.73 +0.6 897,781 - CSW01_18865 0.74 -2.1 897,781 - CSW01_18865 0.74 -2.1 897,781 - CSW01_18865 0.74 -0.5 897,781 - CSW01_18865 0.74 -0.6 897,882 - -0.5 897,924 - +1.1 897,962 - CSW01_18870 0.17 -1.1 898,003 + CSW01_18870 0.34 -3.1 898,013 - CSW01_18870 0.38 +0.6 898,013 - CSW01_18870 0.38 +0.2 898,053 - CSW01_18870 0.54 -2.3 898,097 - CSW01_18870 0.72 +0.5 898,181 - +1.5 898,186 - -2.6 898,186 - +0.5 898,206 + -0.0 898,206 + +0.4 898,251 + +0.0 898,270 - +0.2 898,317 - +0.9 898,319 + +0.9 898,360 + +2.0 898,522 + CSW01_18875 0.36 -1.3 898,569 + CSW01_18875 0.43 -0.6 898,577 + CSW01_18875 0.44 +0.8 898,592 - CSW01_18875 0.47 +0.2 898,624 + CSW01_18875 0.52 +1.0 898,658 + CSW01_18875 0.58 -0.2 898,663 + CSW01_18875 0.58 -2.3 898,671 - CSW01_18875 0.60 +0.3 898,692 + CSW01_18875 0.63 +0.9 898,700 - CSW01_18875 0.64 -1.3 898,700 - CSW01_18875 0.64 +0.7 898,817 + CSW01_18875 0.83 +0.6 898,817 + CSW01_18875 0.83 -1.6 898,824 + CSW01_18875 0.84 -0.8 898,825 - CSW01_18875 0.85 +0.6 898,825 - CSW01_18875 0.85 -0.5 898,825 - CSW01_18875 0.85 +0.5 898,840 - CSW01_18875 0.87 -3.2 899,146 - -2.1 899,263 + CSW01_18880 0.43 +0.7 899,263 + CSW01_18880 0.43 -0.6 899,271 - CSW01_18880 0.45 +1.6 899,279 - CSW01_18880 0.48 -0.1 899,324 - CSW01_18880 0.62 +0.9 899,400 - CSW01_18880 0.85 +0.2 899,424 + -0.4 899,459 - -1.5 899,460 - -2.1 899,460 - -2.3 899,465 + -1.0 899,528 + +0.2 899,532 + +0.6 899,542 + +0.2 899,542 + +0.5 899,542 + +0.4 899,542 + -0.8 899,560 - -0.3 899,560 - -0.2 899,598 + +1.7 899,598 + +1.6 899,598 + -0.0 899,606 - -0.1 899,606 - -1.8 899,650 + +0.6 899,655 + -0.0 899,655 + +1.2 899,720 - CSW01_18885 0.16 -1.1 899,739 + CSW01_18885 0.19 -3.9 899,832 + CSW01_18885 0.32 -2.4 899,840 - CSW01_18885 0.34 +0.4 899,845 - CSW01_18885 0.34 -0.1 899,854 + CSW01_18885 0.36 -1.8 899,882 + CSW01_18885 0.40 +0.3 899,890 - CSW01_18885 0.41 +1.8 899,890 - CSW01_18885 0.41 +0.4
Or see this region's nucleotide sequence