Experiment: ICP1_phage_23.5_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_05770 and CSW01_05775 overlap by 19 nucleotides CSW01_05775 and CSW01_05780 overlap by 4 nucleotides CSW01_05780 and CSW01_05785 are separated by 200 nucleotides
CSW01_05770: CSW01_05770 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,205,039 to 1,205,776
_05770
CSW01_05775: CSW01_05775 - imidazole glycerol phosphate synthase cyclase subunit, at 1,205,758 to 1,206,531
_05775
CSW01_05780: CSW01_05780 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 1,206,528 to 1,207,160
_05780
CSW01_05785: CSW01_05785 - 23S rRNA pseudouridine synthase E, at 1,207,361 to 1,208,086
_05785
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1205.536 kb on - strand, within CSW01_05770 at 1205.550 kb on + strand, within CSW01_05770 at 1205.698 kb on - strand, within CSW01_05770 at 1205.741 kb on + strand at 1205.850 kb on + strand, within CSW01_05775 at 1205.862 kb on + strand, within CSW01_05775 at 1205.876 kb on - strand, within CSW01_05775 at 1205.954 kb on - strand, within CSW01_05775 at 1206.025 kb on + strand, within CSW01_05775 at 1206.078 kb on + strand, within CSW01_05775 at 1206.128 kb on + strand, within CSW01_05775 at 1206.215 kb on + strand, within CSW01_05775 at 1206.253 kb on + strand, within CSW01_05775 at 1206.261 kb on - strand, within CSW01_05775 at 1206.323 kb on + strand, within CSW01_05775 at 1206.331 kb on - strand, within CSW01_05775 at 1206.331 kb on - strand, within CSW01_05775 at 1206.333 kb on + strand, within CSW01_05775 at 1206.333 kb on + strand, within CSW01_05775 at 1206.333 kb on + strand, within CSW01_05775 at 1206.333 kb on + strand, within CSW01_05775 at 1206.341 kb on - strand, within CSW01_05775 at 1206.354 kb on + strand, within CSW01_05775 at 1206.420 kb on - strand, within CSW01_05775 at 1206.447 kb on - strand, within CSW01_05775 at 1206.497 kb on + strand at 1206.505 kb on - strand at 1206.527 kb on + strand at 1206.638 kb on - strand, within CSW01_05780 at 1206.716 kb on - strand, within CSW01_05780 at 1206.725 kb on + strand, within CSW01_05780 at 1206.819 kb on + strand, within CSW01_05780 at 1206.827 kb on + strand, within CSW01_05780 at 1206.835 kb on - strand, within CSW01_05780 at 1206.848 kb on - strand, within CSW01_05780 at 1206.865 kb on + strand, within CSW01_05780 at 1206.884 kb on + strand, within CSW01_05780 at 1206.916 kb on - strand, within CSW01_05780 at 1206.916 kb on - strand, within CSW01_05780 at 1206.966 kb on - strand, within CSW01_05780 at 1207.047 kb on - strand, within CSW01_05780 at 1207.104 kb on + strand at 1207.104 kb on + strand at 1207.112 kb on - strand at 1207.112 kb on - strand at 1207.358 kb on + strand at 1207.366 kb on - strand at 1207.430 kb on + strand at 1207.533 kb on - strand, within CSW01_05785 at 1207.574 kb on - strand, within CSW01_05785 at 1207.604 kb on + strand, within CSW01_05785 at 1207.615 kb on + strand, within CSW01_05785 at 1207.650 kb on - strand, within CSW01_05785 at 1207.650 kb on - strand, within CSW01_05785 at 1207.698 kb on + strand, within CSW01_05785 at 1207.698 kb on + strand, within CSW01_05785 at 1207.706 kb on - strand, within CSW01_05785 at 1207.890 kb on + strand, within CSW01_05785 at 1207.970 kb on + strand, within CSW01_05785 at 1208.054 kb on - strand at 1208.095 kb on - strand at 1208.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep1 remove 1,205,536 - CSW01_05770 0.67 -0.8 1,205,550 + CSW01_05770 0.69 -0.1 1,205,698 - CSW01_05770 0.89 +0.6 1,205,741 + -0.1 1,205,850 + CSW01_05775 0.12 +0.5 1,205,862 + CSW01_05775 0.13 +0.9 1,205,876 - CSW01_05775 0.15 -1.1 1,205,954 - CSW01_05775 0.25 -0.9 1,206,025 + CSW01_05775 0.34 -0.9 1,206,078 + CSW01_05775 0.41 +0.8 1,206,128 + CSW01_05775 0.48 -2.8 1,206,215 + CSW01_05775 0.59 -2.5 1,206,253 + CSW01_05775 0.64 +0.4 1,206,261 - CSW01_05775 0.65 -0.1 1,206,323 + CSW01_05775 0.73 +0.1 1,206,331 - CSW01_05775 0.74 -0.2 1,206,331 - CSW01_05775 0.74 +0.6 1,206,333 + CSW01_05775 0.74 +1.2 1,206,333 + CSW01_05775 0.74 +0.0 1,206,333 + CSW01_05775 0.74 -2.6 1,206,333 + CSW01_05775 0.74 -1.1 1,206,341 - CSW01_05775 0.75 +1.2 1,206,354 + CSW01_05775 0.77 +0.3 1,206,420 - CSW01_05775 0.86 -3.6 1,206,447 - CSW01_05775 0.89 -0.2 1,206,497 + +0.5 1,206,505 - +0.4 1,206,527 + +1.2 1,206,638 - CSW01_05780 0.17 +0.2 1,206,716 - CSW01_05780 0.30 -0.8 1,206,725 + CSW01_05780 0.31 +0.4 1,206,819 + CSW01_05780 0.46 -3.0 1,206,827 + CSW01_05780 0.47 +1.5 1,206,835 - CSW01_05780 0.48 -1.4 1,206,848 - CSW01_05780 0.51 +2.3 1,206,865 + CSW01_05780 0.53 -0.5 1,206,884 + CSW01_05780 0.56 -0.1 1,206,916 - CSW01_05780 0.61 +1.0 1,206,916 - CSW01_05780 0.61 +0.3 1,206,966 - CSW01_05780 0.69 +1.6 1,207,047 - CSW01_05780 0.82 +0.0 1,207,104 + -1.9 1,207,104 + +1.4 1,207,112 - -3.1 1,207,112 - -0.6 1,207,358 + -2.3 1,207,366 - -0.1 1,207,430 + +1.7 1,207,533 - CSW01_05785 0.24 -1.1 1,207,574 - CSW01_05785 0.29 +0.3 1,207,604 + CSW01_05785 0.33 +1.8 1,207,615 + CSW01_05785 0.35 +0.8 1,207,650 - CSW01_05785 0.40 +0.4 1,207,650 - CSW01_05785 0.40 +1.1 1,207,698 + CSW01_05785 0.46 -1.5 1,207,698 + CSW01_05785 0.46 +1.0 1,207,706 - CSW01_05785 0.48 +0.0 1,207,890 + CSW01_05785 0.73 +0.6 1,207,970 + CSW01_05785 0.84 +0.3 1,208,054 - +0.8 1,208,095 - +0.9 1,208,148 - -0.8
Or see this region's nucleotide sequence