Experiment: ICP1_phage_23.5_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_01985 and CSW01_01990 are separated by 160 nucleotides CSW01_01990 and CSW01_01995 are separated by 88 nucleotides CSW01_01995 and CSW01_02000 overlap by 47 nucleotides CSW01_02000 and CSW01_02005 overlap by 43 nucleotides
CSW01_01985: CSW01_01985 - tRNA dihydrouridine(20/20a) synthase DusA, at 393,070 to 394,053
_01985
CSW01_01990: CSW01_01990 - envelope stress response protein PspG, at 394,214 to 394,426
_01990
CSW01_01995: CSW01_01995 - iron-hydroxamate ABC transporter substrate-binding protein, at 394,515 to 395,180
_01995
CSW01_02000: CSW01_02000 - Fe3+ hydroxamate ABC transporter substrate-binding protein, at 395,134 to 395,304
_02000
CSW01_02005: CSW01_02005 - hypothetical protein, at 395,262 to 395,531
_02005
Position (kb)
394
395 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 393.238 kb on - strand, within CSW01_01985 at 393.293 kb on + strand, within CSW01_01985 at 393.333 kb on + strand, within CSW01_01985 at 393.586 kb on + strand, within CSW01_01985 at 393.594 kb on - strand, within CSW01_01985 at 393.690 kb on + strand, within CSW01_01985 at 393.691 kb on + strand, within CSW01_01985 at 393.696 kb on - strand, within CSW01_01985 at 393.735 kb on - strand, within CSW01_01985 at 393.835 kb on + strand, within CSW01_01985 at 393.914 kb on + strand, within CSW01_01985 at 393.914 kb on + strand, within CSW01_01985 at 393.914 kb on + strand, within CSW01_01985 at 393.922 kb on - strand, within CSW01_01985 at 393.922 kb on - strand, within CSW01_01985 at 393.922 kb on - strand, within CSW01_01985 at 393.966 kb on - strand at 393.976 kb on - strand at 393.995 kb on + strand at 394.003 kb on - strand at 394.193 kb on + strand at 394.211 kb on - strand at 394.274 kb on + strand, within CSW01_01990 at 394.274 kb on + strand, within CSW01_01990 at 394.282 kb on - strand, within CSW01_01990 at 394.282 kb on - strand, within CSW01_01990 at 394.291 kb on - strand, within CSW01_01990 at 394.335 kb on - strand, within CSW01_01990 at 394.346 kb on + strand, within CSW01_01990 at 394.346 kb on + strand, within CSW01_01990 at 394.354 kb on - strand, within CSW01_01990 at 394.354 kb on - strand, within CSW01_01990 at 394.354 kb on - strand, within CSW01_01990 at 394.354 kb on - strand, within CSW01_01990 at 394.368 kb on - strand, within CSW01_01990 at 394.416 kb on - strand at 394.432 kb on + strand at 394.455 kb on + strand at 394.504 kb on + strand at 394.512 kb on - strand at 394.544 kb on - strand at 394.549 kb on - strand at 394.589 kb on + strand, within CSW01_01995 at 394.589 kb on + strand, within CSW01_01995 at 394.597 kb on - strand, within CSW01_01995 at 394.597 kb on - strand, within CSW01_01995 at 394.597 kb on - strand, within CSW01_01995 at 394.597 kb on - strand, within CSW01_01995 at 394.599 kb on + strand, within CSW01_01995 at 394.607 kb on - strand, within CSW01_01995 at 394.676 kb on + strand, within CSW01_01995 at 394.677 kb on - strand, within CSW01_01995 at 394.730 kb on - strand, within CSW01_01995 at 394.730 kb on - strand, within CSW01_01995 at 394.816 kb on + strand, within CSW01_01995 at 394.824 kb on - strand, within CSW01_01995 at 394.826 kb on + strand, within CSW01_01995 at 394.834 kb on + strand, within CSW01_01995 at 394.842 kb on - strand, within CSW01_01995 at 394.842 kb on - strand, within CSW01_01995 at 395.083 kb on + strand, within CSW01_01995 at 395.100 kb on - strand, within CSW01_01995 at 395.109 kb on - strand, within CSW01_01995 at 395.119 kb on - strand at 395.161 kb on - strand at 395.317 kb on + strand, within CSW01_02005 at 395.332 kb on + strand, within CSW01_02005 at 395.340 kb on - strand, within CSW01_02005 at 395.361 kb on - strand, within CSW01_02005 at 395.364 kb on - strand, within CSW01_02005 at 395.368 kb on + strand, within CSW01_02005 at 395.376 kb on - strand, within CSW01_02005 at 395.376 kb on - strand, within CSW01_02005
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep1 remove 393,238 - CSW01_01985 0.17 +0.5 393,293 + CSW01_01985 0.23 +0.8 393,333 + CSW01_01985 0.27 -2.1 393,586 + CSW01_01985 0.52 -4.0 393,594 - CSW01_01985 0.53 +0.9 393,690 + CSW01_01985 0.63 -1.3 393,691 + CSW01_01985 0.63 +0.4 393,696 - CSW01_01985 0.64 +1.4 393,735 - CSW01_01985 0.68 +0.5 393,835 + CSW01_01985 0.78 +0.5 393,914 + CSW01_01985 0.86 -1.2 393,914 + CSW01_01985 0.86 +1.5 393,914 + CSW01_01985 0.86 +0.7 393,922 - CSW01_01985 0.87 -1.7 393,922 - CSW01_01985 0.87 -0.7 393,922 - CSW01_01985 0.87 -1.6 393,966 - -0.1 393,976 - +0.1 393,995 + +0.5 394,003 - +0.3 394,193 + +0.7 394,211 - +1.0 394,274 + CSW01_01990 0.28 +0.5 394,274 + CSW01_01990 0.28 -3.7 394,282 - CSW01_01990 0.32 -2.5 394,282 - CSW01_01990 0.32 +0.5 394,291 - CSW01_01990 0.36 +1.1 394,335 - CSW01_01990 0.57 -3.4 394,346 + CSW01_01990 0.62 -1.2 394,346 + CSW01_01990 0.62 +0.4 394,354 - CSW01_01990 0.66 +0.3 394,354 - CSW01_01990 0.66 +0.4 394,354 - CSW01_01990 0.66 +0.5 394,354 - CSW01_01990 0.66 -0.8 394,368 - CSW01_01990 0.72 -2.2 394,416 - +0.4 394,432 + -2.2 394,455 + -0.1 394,504 + +0.4 394,512 - +0.9 394,544 - +0.4 394,549 - -2.0 394,589 + CSW01_01995 0.11 -2.6 394,589 + CSW01_01995 0.11 -0.7 394,597 - CSW01_01995 0.12 -0.2 394,597 - CSW01_01995 0.12 +1.3 394,597 - CSW01_01995 0.12 -3.3 394,597 - CSW01_01995 0.12 -2.0 394,599 + CSW01_01995 0.13 -3.6 394,607 - CSW01_01995 0.14 -1.1 394,676 + CSW01_01995 0.24 -1.3 394,677 - CSW01_01995 0.24 +0.8 394,730 - CSW01_01995 0.32 +0.4 394,730 - CSW01_01995 0.32 -0.1 394,816 + CSW01_01995 0.45 -0.1 394,824 - CSW01_01995 0.46 -2.6 394,826 + CSW01_01995 0.47 -4.2 394,834 + CSW01_01995 0.48 -3.9 394,842 - CSW01_01995 0.49 -1.6 394,842 - CSW01_01995 0.49 -3.6 395,083 + CSW01_01995 0.85 +0.4 395,100 - CSW01_01995 0.88 +0.4 395,109 - CSW01_01995 0.89 -2.0 395,119 - -0.6 395,161 - +1.4 395,317 + CSW01_02005 0.20 -3.8 395,332 + CSW01_02005 0.26 +0.0 395,340 - CSW01_02005 0.29 -1.1 395,361 - CSW01_02005 0.37 -1.5 395,364 - CSW01_02005 0.38 -0.4 395,368 + CSW01_02005 0.39 -0.8 395,376 - CSW01_02005 0.42 -4.1 395,376 - CSW01_02005 0.42 +0.1
Or see this region's nucleotide sequence