Experiment: 1-ethyl-3-methylimidazolium lysine 10.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_04490 and AO353_04495 are separated by 124 nucleotides AO353_04495 and AO353_04500 are separated by 49 nucleotides AO353_04500 and AO353_04505 are separated by 201 nucleotides
AO353_04490: AO353_04490 - murein transglycosylase, at 968,939 to 970,399
_04490
AO353_04495: AO353_04495 - pyridoxine 5'-phosphate synthase, at 970,524 to 971,267
_04495
AO353_04500: AO353_04500 - DNA repair protein RecO, at 971,317 to 972,006
_04500
AO353_04505: AO353_04505 - GTPase Era, at 972,208 to 973,110
_04505
Position (kb) 971
972
973 Strain fitness (log2 ratio) -2
-1
0
1
2 at 970.397 kb on + strand at 970.398 kb on - strand at 971.307 kb on + strand at 971.307 kb on + strand at 971.308 kb on - strand at 971.486 kb on + strand, within AO353_04500 at 971.486 kb on + strand, within AO353_04500 at 971.541 kb on + strand, within AO353_04500 at 971.669 kb on + strand, within AO353_04500 at 971.735 kb on + strand, within AO353_04500 at 971.736 kb on - strand, within AO353_04500 at 971.736 kb on - strand, within AO353_04500 at 971.941 kb on - strand at 971.941 kb on - strand at 971.961 kb on + strand at 971.972 kb on + strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 972.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10.5 mM remove 970,397 + -0.6 970,398 - -1.4 971,307 + +1.1 971,307 + +2.2 971,308 - -0.3 971,486 + AO353_04500 0.24 -0.9 971,486 + AO353_04500 0.24 -1.1 971,541 + AO353_04500 0.32 -1.7 971,669 + AO353_04500 0.51 -0.3 971,735 + AO353_04500 0.61 -1.7 971,736 - AO353_04500 0.61 +1.1 971,736 - AO353_04500 0.61 -0.9 971,941 - -0.9 971,941 - +0.5 971,961 + -1.8 971,972 + +0.3 971,973 - +1.1 971,973 - -0.4 971,973 - +0.3 971,973 - +2.7 971,973 - +0.9 972,210 - +0.2
Or see this region's nucleotide sequence