Experiment: Cephalothin 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispG and amiA are separated by 70 nucleotides amiA and tRNA-Leu are separated by 158 nucleotides tRNA-Leu and murZ are separated by 299 nucleotides
Synpcc7942_0713: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 706,450 to 707,673
ispG
Synpcc7942_0714: amiA - Cell wall hydrolase/autolysin, at 707,744 to 709,450
amiA
Synpcc7942_R0039: tRNA-Leu - tRNA-Leu, at 709,609 to 709,692
tRNA-Leu
Synpcc7942_0715: murZ - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 709,992 to 711,179
murZ
Position (kb) 707
708
709
710 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 707.665 kb on + strand at 707.666 kb on + strand at 707.678 kb on - strand at 707.742 kb on - strand at 707.750 kb on - strand at 707.750 kb on - strand at 707.751 kb on + strand at 707.768 kb on + strand at 707.773 kb on + strand at 707.776 kb on - strand at 707.782 kb on - strand at 707.788 kb on + strand at 707.850 kb on + strand at 707.861 kb on + strand at 707.863 kb on - strand at 707.869 kb on + strand at 707.876 kb on + strand at 707.878 kb on + strand at 707.883 kb on + strand at 707.889 kb on + strand at 707.891 kb on - strand at 707.899 kb on + strand at 707.902 kb on - strand at 707.907 kb on - strand at 707.930 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.957 kb on + strand, within amiA at 707.965 kb on - strand, within amiA at 707.965 kb on - strand, within amiA at 707.965 kb on - strand at 707.965 kb on - strand, within amiA at 707.965 kb on - strand, within amiA at 707.965 kb on - strand, within amiA at 707.965 kb on - strand, within amiA at 708.047 kb on - strand, within amiA at 708.075 kb on + strand, within amiA at 708.090 kb on + strand, within amiA at 708.095 kb on + strand, within amiA at 708.095 kb on + strand, within amiA at 708.135 kb on + strand, within amiA at 708.135 kb on + strand, within amiA at 708.136 kb on + strand, within amiA at 708.146 kb on + strand, within amiA at 708.146 kb on + strand, within amiA at 708.154 kb on - strand, within amiA at 708.159 kb on + strand, within amiA at 708.169 kb on + strand, within amiA at 708.191 kb on + strand, within amiA at 708.199 kb on - strand, within amiA at 708.199 kb on - strand, within amiA at 708.208 kb on - strand, within amiA at 708.208 kb on - strand, within amiA at 708.258 kb on + strand, within amiA at 708.271 kb on - strand, within amiA at 708.271 kb on - strand, within amiA at 708.276 kb on - strand, within amiA at 708.304 kb on - strand, within amiA at 708.304 kb on - strand, within amiA at 708.352 kb on + strand, within amiA at 708.377 kb on - strand, within amiA at 708.387 kb on - strand, within amiA at 708.415 kb on + strand, within amiA at 708.416 kb on + strand, within amiA at 708.416 kb on - strand, within amiA at 708.440 kb on + strand, within amiA at 708.440 kb on + strand, within amiA at 708.440 kb on + strand, within amiA at 708.444 kb on - strand, within amiA at 708.448 kb on - strand, within amiA at 708.448 kb on - strand, within amiA at 708.482 kb on - strand, within amiA at 708.485 kb on - strand, within amiA at 708.485 kb on - strand, within amiA at 708.485 kb on - strand, within amiA at 708.485 kb on - strand, within amiA at 708.520 kb on + strand, within amiA at 708.528 kb on - strand, within amiA at 708.528 kb on - strand, within amiA at 708.534 kb on - strand, within amiA at 708.580 kb on - strand, within amiA at 708.654 kb on - strand, within amiA at 708.670 kb on + strand, within amiA at 708.693 kb on + strand, within amiA at 708.732 kb on - strand, within amiA at 708.747 kb on - strand, within amiA at 708.754 kb on - strand, within amiA at 708.769 kb on - strand, within amiA at 708.780 kb on + strand, within amiA at 708.782 kb on + strand, within amiA at 708.788 kb on - strand, within amiA at 708.788 kb on - strand, within amiA at 708.790 kb on + strand, within amiA at 708.798 kb on - strand, within amiA at 708.805 kb on + strand, within amiA at 708.813 kb on - strand, within amiA at 708.829 kb on + strand, within amiA at 708.831 kb on + strand at 708.831 kb on + strand, within amiA at 708.831 kb on + strand, within amiA at 708.831 kb on + strand, within amiA at 708.831 kb on + strand, within amiA at 708.831 kb on + strand, within amiA at 708.839 kb on - strand, within amiA at 708.839 kb on - strand, within amiA at 708.839 kb on - strand, within amiA at 708.839 kb on - strand, within amiA at 708.839 kb on - strand, within amiA at 708.877 kb on + strand, within amiA at 708.935 kb on + strand, within amiA at 708.950 kb on - strand, within amiA at 708.955 kb on - strand, within amiA at 708.976 kb on - strand, within amiA at 709.001 kb on + strand, within amiA at 709.001 kb on + strand, within amiA at 709.001 kb on + strand, within amiA at 709.001 kb on + strand, within amiA at 709.009 kb on - strand, within amiA at 709.011 kb on + strand, within amiA at 709.037 kb on + strand at 709.045 kb on - strand, within amiA at 709.077 kb on + strand, within amiA at 709.110 kb on + strand, within amiA at 709.126 kb on + strand, within amiA at 709.126 kb on + strand, within amiA at 709.126 kb on + strand, within amiA at 709.134 kb on - strand, within amiA at 709.134 kb on - strand, within amiA at 709.136 kb on + strand, within amiA at 709.148 kb on - strand, within amiA at 709.163 kb on - strand, within amiA at 709.183 kb on + strand, within amiA at 709.217 kb on + strand, within amiA at 709.230 kb on - strand, within amiA at 709.232 kb on - strand, within amiA at 709.232 kb on - strand, within amiA at 709.266 kb on - strand, within amiA at 709.305 kb on + strand at 709.310 kb on + strand at 709.310 kb on + strand at 709.310 kb on + strand at 709.315 kb on + strand at 709.315 kb on + strand at 709.342 kb on - strand at 709.389 kb on + strand at 709.392 kb on + strand at 709.440 kb on + strand at 709.440 kb on + strand at 709.448 kb on - strand at 709.469 kb on - strand at 709.492 kb on - strand at 709.492 kb on - strand at 709.492 kb on - strand at 709.524 kb on - strand at 709.524 kb on - strand at 709.524 kb on - strand at 709.530 kb on + strand at 709.532 kb on + strand at 709.538 kb on - strand at 709.538 kb on - strand at 709.538 kb on - strand at 709.538 kb on - strand at 709.575 kb on + strand at 709.760 kb on - strand at 709.770 kb on + strand at 709.770 kb on + strand at 709.772 kb on + strand at 709.775 kb on + strand at 709.775 kb on + strand at 709.775 kb on + strand at 709.775 kb on + strand at 709.775 kb on + strand at 709.783 kb on - strand at 709.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.00390625 mg/ml remove 707,665 + +2.8 707,666 + +1.8 707,678 - -3.1 707,742 - -0.4 707,750 - -2.3 707,750 - -1.1 707,751 + +0.0 707,768 + -0.7 707,773 + -0.7 707,776 - -2.0 707,782 - -0.5 707,788 + -2.2 707,850 + -0.2 707,861 + -2.7 707,863 - -0.2 707,869 + -2.3 707,876 + -2.7 707,878 + -0.7 707,883 + -2.2 707,889 + -0.0 707,891 - -2.6 707,899 + -0.5 707,902 - -3.6 707,907 - -1.7 707,930 + amiA Synpcc7942_0714 0.11 +1.4 707,957 + amiA Synpcc7942_0714 0.12 -1.5 707,957 + amiA Synpcc7942_0714 0.12 -0.8 707,957 + amiA Synpcc7942_0714 0.12 +1.5 707,957 + amiA Synpcc7942_0714 0.12 -2.3 707,957 + amiA Synpcc7942_0714 0.12 -2.3 707,957 + amiA Synpcc7942_0714 0.12 -1.2 707,957 + amiA Synpcc7942_0714 0.12 -3.1 707,957 + amiA Synpcc7942_0714 0.12 -3.2 707,957 + amiA Synpcc7942_0714 0.12 -4.1 707,965 - amiA Synpcc7942_0714 0.13 -1.2 707,965 - amiA Synpcc7942_0714 0.13 -2.9 707,965 - +0.8 707,965 - amiA Synpcc7942_0714 0.13 -2.7 707,965 - amiA Synpcc7942_0714 0.13 -1.9 707,965 - amiA Synpcc7942_0714 0.13 -3.0 707,965 - amiA Synpcc7942_0714 0.13 -1.5 708,047 - amiA Synpcc7942_0714 0.18 -3.5 708,075 + amiA Synpcc7942_0714 0.19 -1.2 708,090 + amiA Synpcc7942_0714 0.20 -2.0 708,095 + amiA Synpcc7942_0714 0.21 -2.5 708,095 + amiA Synpcc7942_0714 0.21 -1.2 708,135 + amiA Synpcc7942_0714 0.23 -2.5 708,135 + amiA Synpcc7942_0714 0.23 -3.2 708,136 + amiA Synpcc7942_0714 0.23 -0.1 708,146 + amiA Synpcc7942_0714 0.24 -1.5 708,146 + amiA Synpcc7942_0714 0.24 -2.6 708,154 - amiA Synpcc7942_0714 0.24 -1.5 708,159 + amiA Synpcc7942_0714 0.24 -1.5 708,169 + amiA Synpcc7942_0714 0.25 -1.2 708,191 + amiA Synpcc7942_0714 0.26 +0.8 708,199 - amiA Synpcc7942_0714 0.27 -3.2 708,199 - amiA Synpcc7942_0714 0.27 +0.4 708,208 - amiA Synpcc7942_0714 0.27 -2.9 708,208 - amiA Synpcc7942_0714 0.27 -2.0 708,258 + amiA Synpcc7942_0714 0.30 -1.5 708,271 - amiA Synpcc7942_0714 0.31 -0.2 708,271 - amiA Synpcc7942_0714 0.31 -2.5 708,276 - amiA Synpcc7942_0714 0.31 -0.7 708,304 - amiA Synpcc7942_0714 0.33 -3.3 708,304 - amiA Synpcc7942_0714 0.33 -1.5 708,352 + amiA Synpcc7942_0714 0.36 -3.1 708,377 - amiA Synpcc7942_0714 0.37 -2.6 708,387 - amiA Synpcc7942_0714 0.38 -4.0 708,415 + amiA Synpcc7942_0714 0.39 -1.6 708,416 + amiA Synpcc7942_0714 0.39 -0.7 708,416 - amiA Synpcc7942_0714 0.39 -2.3 708,440 + amiA Synpcc7942_0714 0.41 +1.8 708,440 + amiA Synpcc7942_0714 0.41 +0.3 708,440 + amiA Synpcc7942_0714 0.41 -2.6 708,444 - amiA Synpcc7942_0714 0.41 -0.2 708,448 - amiA Synpcc7942_0714 0.41 -1.2 708,448 - amiA Synpcc7942_0714 0.41 +1.8 708,482 - amiA Synpcc7942_0714 0.43 -0.2 708,485 - amiA Synpcc7942_0714 0.43 -3.0 708,485 - amiA Synpcc7942_0714 0.43 -2.0 708,485 - amiA Synpcc7942_0714 0.43 -0.2 708,485 - amiA Synpcc7942_0714 0.43 -2.3 708,520 + amiA Synpcc7942_0714 0.45 -0.7 708,528 - amiA Synpcc7942_0714 0.46 -2.2 708,528 - amiA Synpcc7942_0714 0.46 +1.0 708,534 - amiA Synpcc7942_0714 0.46 +3.5 708,580 - amiA Synpcc7942_0714 0.49 -1.2 708,654 - amiA Synpcc7942_0714 0.53 -2.3 708,670 + amiA Synpcc7942_0714 0.54 -2.5 708,693 + amiA Synpcc7942_0714 0.56 +0.2 708,732 - amiA Synpcc7942_0714 0.58 -2.0 708,747 - amiA Synpcc7942_0714 0.59 -2.5 708,754 - amiA Synpcc7942_0714 0.59 -3.7 708,769 - amiA Synpcc7942_0714 0.60 -0.5 708,780 + amiA Synpcc7942_0714 0.61 -2.9 708,782 + amiA Synpcc7942_0714 0.61 -3.7 708,788 - amiA Synpcc7942_0714 0.61 -1.2 708,788 - amiA Synpcc7942_0714 0.61 +1.1 708,790 + amiA Synpcc7942_0714 0.61 -1.7 708,798 - amiA Synpcc7942_0714 0.62 +0.2 708,805 + amiA Synpcc7942_0714 0.62 -2.0 708,813 - amiA Synpcc7942_0714 0.63 +0.8 708,829 + amiA Synpcc7942_0714 0.64 -2.7 708,831 + +0.8 708,831 + amiA Synpcc7942_0714 0.64 -2.2 708,831 + amiA Synpcc7942_0714 0.64 -1.5 708,831 + amiA Synpcc7942_0714 0.64 -0.2 708,831 + amiA Synpcc7942_0714 0.64 -0.7 708,831 + amiA Synpcc7942_0714 0.64 -2.3 708,839 - amiA Synpcc7942_0714 0.64 -0.2 708,839 - amiA Synpcc7942_0714 0.64 -1.2 708,839 - amiA Synpcc7942_0714 0.64 -0.6 708,839 - amiA Synpcc7942_0714 0.64 -0.7 708,839 - amiA Synpcc7942_0714 0.64 -2.2 708,877 + amiA Synpcc7942_0714 0.66 -2.0 708,935 + amiA Synpcc7942_0714 0.70 -1.7 708,950 - amiA Synpcc7942_0714 0.71 -2.7 708,955 - amiA Synpcc7942_0714 0.71 -0.2 708,976 - amiA Synpcc7942_0714 0.72 -3.0 709,001 + amiA Synpcc7942_0714 0.74 -1.5 709,001 + amiA Synpcc7942_0714 0.74 -2.5 709,001 + amiA Synpcc7942_0714 0.74 -3.2 709,001 + amiA Synpcc7942_0714 0.74 +2.1 709,009 - amiA Synpcc7942_0714 0.74 -0.1 709,011 + amiA Synpcc7942_0714 0.74 -1.7 709,037 + -0.7 709,045 - amiA Synpcc7942_0714 0.76 -0.2 709,077 + amiA Synpcc7942_0714 0.78 -0.2 709,110 + amiA Synpcc7942_0714 0.80 -2.5 709,126 + amiA Synpcc7942_0714 0.81 -1.0 709,126 + amiA Synpcc7942_0714 0.81 -0.2 709,126 + amiA Synpcc7942_0714 0.81 -4.4 709,134 - amiA Synpcc7942_0714 0.81 -3.7 709,134 - amiA Synpcc7942_0714 0.81 +0.8 709,136 + amiA Synpcc7942_0714 0.82 -1.9 709,148 - amiA Synpcc7942_0714 0.82 +1.6 709,163 - amiA Synpcc7942_0714 0.83 -2.3 709,183 + amiA Synpcc7942_0714 0.84 -0.6 709,217 + amiA Synpcc7942_0714 0.86 -0.7 709,230 - amiA Synpcc7942_0714 0.87 +0.4 709,232 - amiA Synpcc7942_0714 0.87 -0.6 709,232 - amiA Synpcc7942_0714 0.87 +1.3 709,266 - amiA Synpcc7942_0714 0.89 -2.2 709,305 + +0.8 709,310 + -2.3 709,310 + -2.0 709,310 + -3.1 709,315 + -2.7 709,315 + -2.0 709,342 - +2.2 709,389 + +0.8 709,392 + -1.2 709,440 + +0.3 709,440 + -0.2 709,448 - -0.5 709,469 - +1.3 709,492 - +0.6 709,492 - +1.0 709,492 - -0.9 709,524 - +2.7 709,524 - +0.9 709,524 - -0.7 709,530 + -0.7 709,532 + -0.2 709,538 - +3.0 709,538 - +0.8 709,538 - -0.7 709,538 - -1.5 709,575 + -0.4 709,760 - -1.5 709,770 + -1.7 709,770 + +0.4 709,772 + -2.2 709,775 + -0.5 709,775 + +0.8 709,775 + -2.0 709,775 + -1.5 709,775 + -1.0 709,783 - -1.7 709,812 + -0.7
Or see this region's nucleotide sequence