Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36965 and RR42_RS36970 are separated by 5 nucleotides RR42_RS36970 and RR42_RS36975 are separated by 177 nucleotides RR42_RS36975 and RR42_RS36980 are separated by 22 nucleotides
RR42_RS36965: RR42_RS36965 - cellulose synthase, at 3,748,145 to 3,750,130
_RS36965
RR42_RS36970: RR42_RS36970 - RND transporter, at 3,750,136 to 3,751,581
_RS36970
RR42_RS36975: RR42_RS36975 - polar amino acid ABC transporter permease, at 3,751,759 to 3,752,394
_RS36975
RR42_RS36980: RR42_RS36980 - amino acid ABC transporter permease, at 3,752,417 to 3,753,091
_RS36980
Position (kb)
3750
3751
3752 Strain fitness (log2 ratio)
-2
-1
0
1 at 3749.153 kb on + strand, within RR42_RS36965 at 3749.258 kb on + strand, within RR42_RS36965 at 3749.259 kb on - strand, within RR42_RS36965 at 3749.327 kb on + strand, within RR42_RS36965 at 3749.327 kb on + strand, within RR42_RS36965 at 3749.328 kb on - strand, within RR42_RS36965 at 3749.328 kb on - strand, within RR42_RS36965 at 3749.328 kb on - strand, within RR42_RS36965 at 3749.333 kb on + strand, within RR42_RS36965 at 3749.334 kb on - strand, within RR42_RS36965 at 3749.603 kb on + strand, within RR42_RS36965 at 3749.604 kb on - strand, within RR42_RS36965 at 3749.838 kb on - strand, within RR42_RS36965 at 3749.848 kb on + strand, within RR42_RS36965 at 3750.220 kb on + strand at 3750.220 kb on + strand at 3750.221 kb on - strand at 3750.221 kb on - strand at 3750.221 kb on - strand at 3750.272 kb on - strand at 3750.637 kb on + strand, within RR42_RS36970 at 3750.638 kb on - strand, within RR42_RS36970 at 3750.638 kb on - strand, within RR42_RS36970 at 3750.638 kb on - strand, within RR42_RS36970 at 3750.672 kb on + strand, within RR42_RS36970 at 3750.672 kb on + strand, within RR42_RS36970 at 3750.672 kb on + strand, within RR42_RS36970 at 3750.704 kb on - strand, within RR42_RS36970 at 3750.877 kb on + strand, within RR42_RS36970 at 3750.877 kb on + strand, within RR42_RS36970 at 3750.877 kb on + strand, within RR42_RS36970 at 3750.878 kb on - strand, within RR42_RS36970 at 3751.004 kb on + strand, within RR42_RS36970 at 3751.275 kb on + strand, within RR42_RS36970 at 3751.275 kb on + strand, within RR42_RS36970 at 3751.275 kb on + strand, within RR42_RS36970 at 3751.275 kb on + strand, within RR42_RS36970 at 3751.276 kb on - strand, within RR42_RS36970 at 3751.348 kb on + strand, within RR42_RS36970 at 3751.349 kb on - strand, within RR42_RS36970 at 3751.349 kb on - strand, within RR42_RS36970 at 3751.444 kb on + strand at 3751.444 kb on + strand at 3751.445 kb on - strand at 3751.445 kb on - strand at 3751.477 kb on + strand at 3751.477 kb on + strand at 3751.540 kb on + strand at 3751.541 kb on - strand at 3751.541 kb on - strand at 3751.648 kb on + strand at 3751.648 kb on + strand at 3751.649 kb on - strand at 3751.793 kb on + strand at 3751.829 kb on + strand, within RR42_RS36975 at 3751.891 kb on - strand, within RR42_RS36975 at 3751.892 kb on + strand, within RR42_RS36975 at 3751.892 kb on + strand, within RR42_RS36975 at 3751.893 kb on - strand, within RR42_RS36975 at 3751.905 kb on + strand, within RR42_RS36975 at 3751.905 kb on + strand, within RR42_RS36975 at 3751.905 kb on + strand, within RR42_RS36975 at 3752.078 kb on + strand, within RR42_RS36975 at 3752.162 kb on + strand, within RR42_RS36975 at 3752.499 kb on + strand, within RR42_RS36980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,749,153 + RR42_RS36965 0.51 -0.3 3,749,258 + RR42_RS36965 0.56 +1.2 3,749,259 - RR42_RS36965 0.56 -0.6 3,749,327 + RR42_RS36965 0.60 -0.7 3,749,327 + RR42_RS36965 0.60 -0.5 3,749,328 - RR42_RS36965 0.60 -0.1 3,749,328 - RR42_RS36965 0.60 +0.0 3,749,328 - RR42_RS36965 0.60 -0.8 3,749,333 + RR42_RS36965 0.60 -0.1 3,749,334 - RR42_RS36965 0.60 +0.0 3,749,603 + RR42_RS36965 0.73 +0.1 3,749,604 - RR42_RS36965 0.73 -0.1 3,749,838 - RR42_RS36965 0.85 -0.5 3,749,848 + RR42_RS36965 0.86 -0.7 3,750,220 + +0.4 3,750,220 + -0.2 3,750,221 - +0.2 3,750,221 - -1.9 3,750,221 - -0.0 3,750,272 - -2.3 3,750,637 + RR42_RS36970 0.35 +0.9 3,750,638 - RR42_RS36970 0.35 -0.8 3,750,638 - RR42_RS36970 0.35 -0.3 3,750,638 - RR42_RS36970 0.35 -0.1 3,750,672 + RR42_RS36970 0.37 +0.3 3,750,672 + RR42_RS36970 0.37 +1.3 3,750,672 + RR42_RS36970 0.37 -0.0 3,750,704 - RR42_RS36970 0.39 -1.2 3,750,877 + RR42_RS36970 0.51 -0.4 3,750,877 + RR42_RS36970 0.51 +0.7 3,750,877 + RR42_RS36970 0.51 +1.1 3,750,878 - RR42_RS36970 0.51 -0.0 3,751,004 + RR42_RS36970 0.60 +1.0 3,751,275 + RR42_RS36970 0.79 +0.8 3,751,275 + RR42_RS36970 0.79 -0.1 3,751,275 + RR42_RS36970 0.79 -2.1 3,751,275 + RR42_RS36970 0.79 -1.2 3,751,276 - RR42_RS36970 0.79 -1.1 3,751,348 + RR42_RS36970 0.84 -0.0 3,751,349 - RR42_RS36970 0.84 +0.5 3,751,349 - RR42_RS36970 0.84 -0.0 3,751,444 + +0.4 3,751,444 + +0.1 3,751,445 - +0.3 3,751,445 - -0.2 3,751,477 + -0.3 3,751,477 + -0.2 3,751,540 + +0.1 3,751,541 - +0.3 3,751,541 - -0.4 3,751,648 + -0.1 3,751,648 + -0.9 3,751,649 - +0.5 3,751,793 + +0.6 3,751,829 + RR42_RS36975 0.11 -0.3 3,751,891 - RR42_RS36975 0.21 +0.2 3,751,892 + RR42_RS36975 0.21 +0.3 3,751,892 + RR42_RS36975 0.21 -2.6 3,751,893 - RR42_RS36975 0.21 -0.7 3,751,905 + RR42_RS36975 0.23 -0.5 3,751,905 + RR42_RS36975 0.23 -0.3 3,751,905 + RR42_RS36975 0.23 -1.1 3,752,078 + RR42_RS36975 0.50 -0.8 3,752,162 + RR42_RS36975 0.63 -0.9 3,752,499 + RR42_RS36980 0.12 -0.1
Or see this region's nucleotide sequence