Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36240 and RR42_RS36245 are separated by 6 nucleotides RR42_RS36245 and RR42_RS36250 are separated by 109 nucleotides
RR42_RS36240: RR42_RS36240 - amidohydrolase, at 3,579,560 to 3,581,029
_RS36240
RR42_RS36245: RR42_RS36245 - excisionase, at 3,581,036 to 3,582,214
_RS36245
RR42_RS36250: RR42_RS36250 - hypothetical protein, at 3,582,324 to 3,583,742
_RS36250
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3580.226 kb on + strand, within RR42_RS36240 at 3580.226 kb on + strand, within RR42_RS36240 at 3580.227 kb on - strand, within RR42_RS36240 at 3580.344 kb on - strand, within RR42_RS36240 at 3580.805 kb on + strand, within RR42_RS36240 at 3581.058 kb on + strand at 3581.059 kb on - strand at 3581.059 kb on - strand at 3581.280 kb on - strand, within RR42_RS36245 at 3581.765 kb on - strand, within RR42_RS36245 at 3582.087 kb on + strand, within RR42_RS36245 at 3582.144 kb on + strand at 3582.144 kb on + strand at 3582.144 kb on + strand at 3582.144 kb on + strand at 3582.145 kb on - strand at 3582.145 kb on - strand at 3582.145 kb on - strand at 3582.145 kb on - strand at 3582.145 kb on - strand at 3582.150 kb on + strand at 3582.150 kb on + strand at 3582.150 kb on + strand at 3582.150 kb on + strand at 3582.150 kb on + strand at 3582.150 kb on + strand at 3582.151 kb on - strand at 3582.151 kb on - strand at 3582.151 kb on - strand at 3582.266 kb on - strand at 3582.279 kb on + strand at 3582.279 kb on + strand at 3582.279 kb on + strand at 3582.557 kb on + strand, within RR42_RS36250 at 3582.557 kb on + strand, within RR42_RS36250 at 3582.558 kb on - strand, within RR42_RS36250 at 3582.558 kb on - strand, within RR42_RS36250 at 3582.595 kb on + strand, within RR42_RS36250 at 3582.667 kb on - strand, within RR42_RS36250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,580,226 + RR42_RS36240 0.45 -1.4 3,580,226 + RR42_RS36240 0.45 -0.1 3,580,227 - RR42_RS36240 0.45 +0.5 3,580,344 - RR42_RS36240 0.53 -1.6 3,580,805 + RR42_RS36240 0.85 +0.9 3,581,058 + +0.3 3,581,059 - -1.3 3,581,059 - -0.7 3,581,280 - RR42_RS36245 0.21 +0.1 3,581,765 - RR42_RS36245 0.62 -0.3 3,582,087 + RR42_RS36245 0.89 +0.4 3,582,144 + -0.4 3,582,144 + -0.3 3,582,144 + +0.4 3,582,144 + +0.6 3,582,145 - +0.3 3,582,145 - -1.7 3,582,145 - +0.1 3,582,145 - +0.2 3,582,145 - -1.7 3,582,150 + -2.1 3,582,150 + +1.4 3,582,150 + +0.3 3,582,150 + -0.4 3,582,150 + +0.2 3,582,150 + -1.0 3,582,151 - -0.3 3,582,151 - +0.3 3,582,151 - +0.6 3,582,266 - -0.1 3,582,279 + +0.4 3,582,279 + +2.4 3,582,279 + +1.7 3,582,557 + RR42_RS36250 0.16 +0.0 3,582,557 + RR42_RS36250 0.16 -0.6 3,582,558 - RR42_RS36250 0.16 -1.2 3,582,558 - RR42_RS36250 0.16 -0.3 3,582,595 + RR42_RS36250 0.19 +0.6 3,582,667 - RR42_RS36250 0.24 -1.0
Or see this region's nucleotide sequence