Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36035 and RR42_RS36040 are separated by 185 nucleotides RR42_RS36040 and RR42_RS36045 are separated by 143 nucleotides RR42_RS36045 and RR42_RS36050 overlap by 32 nucleotides
RR42_RS36035: RR42_RS36035 - TonB-dependent receptor, at 3,533,159 to 3,535,447
_RS36035
RR42_RS36040: RR42_RS36040 - TonB-dependent receptor, at 3,535,633 to 3,537,918
_RS36040
RR42_RS36045: RR42_RS36045 - hypothetical protein, at 3,538,062 to 3,538,850
_RS36045
RR42_RS36050: RR42_RS36050 - hypothetical protein, at 3,538,819 to 3,539,025
_RS36050
Position (kb)
3535
3536
3537
3538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3534.683 kb on - strand, within RR42_RS36035 at 3534.708 kb on - strand, within RR42_RS36035 at 3534.725 kb on + strand, within RR42_RS36035 at 3534.752 kb on + strand, within RR42_RS36035 at 3534.752 kb on + strand, within RR42_RS36035 at 3534.752 kb on + strand, within RR42_RS36035 at 3534.753 kb on - strand, within RR42_RS36035 at 3534.753 kb on - strand, within RR42_RS36035 at 3534.753 kb on - strand, within RR42_RS36035 at 3534.761 kb on + strand, within RR42_RS36035 at 3535.180 kb on + strand, within RR42_RS36035 at 3535.181 kb on - strand, within RR42_RS36035 at 3535.184 kb on + strand, within RR42_RS36035 at 3535.184 kb on + strand, within RR42_RS36035 at 3535.184 kb on + strand, within RR42_RS36035 at 3535.184 kb on + strand, within RR42_RS36035 at 3535.185 kb on - strand, within RR42_RS36035 at 3535.223 kb on + strand at 3535.223 kb on + strand at 3535.224 kb on - strand at 3535.224 kb on - strand at 3535.226 kb on + strand at 3535.226 kb on + strand at 3535.226 kb on + strand at 3535.226 kb on + strand at 3535.227 kb on - strand at 3535.304 kb on + strand at 3535.364 kb on + strand at 3535.365 kb on - strand at 3535.367 kb on + strand at 3535.367 kb on + strand at 3535.368 kb on - strand at 3535.368 kb on - strand at 3535.368 kb on - strand at 3535.368 kb on - strand at 3535.368 kb on - strand at 3535.391 kb on + strand at 3535.392 kb on - strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.500 kb on + strand at 3535.501 kb on - strand at 3535.501 kb on - strand at 3535.501 kb on - strand at 3535.501 kb on - strand at 3535.501 kb on - strand at 3535.501 kb on - strand at 3535.543 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.556 kb on + strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.557 kb on - strand at 3535.575 kb on - strand at 3536.009 kb on - strand, within RR42_RS36040 at 3536.290 kb on + strand, within RR42_RS36040 at 3536.291 kb on - strand, within RR42_RS36040 at 3536.398 kb on + strand, within RR42_RS36040 at 3536.398 kb on + strand, within RR42_RS36040 at 3536.399 kb on - strand, within RR42_RS36040 at 3536.401 kb on + strand, within RR42_RS36040 at 3536.402 kb on - strand, within RR42_RS36040 at 3536.402 kb on - strand, within RR42_RS36040 at 3536.402 kb on - strand, within RR42_RS36040 at 3536.443 kb on + strand, within RR42_RS36040 at 3536.536 kb on + strand, within RR42_RS36040 at 3536.536 kb on + strand, within RR42_RS36040 at 3536.536 kb on + strand, within RR42_RS36040 at 3536.536 kb on + strand, within RR42_RS36040 at 3536.537 kb on - strand, within RR42_RS36040 at 3536.540 kb on - strand, within RR42_RS36040 at 3536.644 kb on - strand, within RR42_RS36040 at 3536.665 kb on + strand, within RR42_RS36040 at 3536.666 kb on - strand, within RR42_RS36040 at 3536.666 kb on - strand, within RR42_RS36040 at 3536.666 kb on - strand, within RR42_RS36040 at 3536.704 kb on + strand, within RR42_RS36040 at 3536.707 kb on + strand, within RR42_RS36040 at 3536.707 kb on + strand, within RR42_RS36040 at 3536.815 kb on + strand, within RR42_RS36040 at 3536.863 kb on + strand, within RR42_RS36040 at 3536.863 kb on + strand, within RR42_RS36040 at 3536.864 kb on - strand, within RR42_RS36040 at 3536.864 kb on - strand, within RR42_RS36040 at 3536.864 kb on - strand, within RR42_RS36040 at 3536.920 kb on + strand, within RR42_RS36040 at 3536.938 kb on + strand, within RR42_RS36040 at 3536.939 kb on - strand, within RR42_RS36040 at 3537.026 kb on - strand, within RR42_RS36040 at 3537.091 kb on + strand, within RR42_RS36040 at 3537.169 kb on + strand, within RR42_RS36040 at 3537.169 kb on + strand, within RR42_RS36040 at 3537.187 kb on + strand, within RR42_RS36040 at 3537.187 kb on + strand, within RR42_RS36040 at 3537.187 kb on + strand, within RR42_RS36040 at 3537.214 kb on + strand, within RR42_RS36040 at 3537.540 kb on + strand, within RR42_RS36040 at 3537.540 kb on + strand, within RR42_RS36040 at 3537.540 kb on + strand, within RR42_RS36040 at 3537.540 kb on + strand, within RR42_RS36040 at 3537.541 kb on - strand, within RR42_RS36040 at 3537.655 kb on + strand, within RR42_RS36040 at 3537.694 kb on + strand at 3537.697 kb on + strand at 3537.697 kb on + strand at 3537.698 kb on - strand at 3537.698 kb on - strand at 3537.835 kb on + strand at 3537.835 kb on + strand at 3537.851 kb on - strand at 3537.913 kb on + strand at 3538.387 kb on - strand, within RR42_RS36045 at 3538.425 kb on + strand, within RR42_RS36045 at 3538.426 kb on - strand, within RR42_RS36045 at 3538.494 kb on + strand, within RR42_RS36045 at 3538.494 kb on + strand, within RR42_RS36045 at 3538.551 kb on + strand, within RR42_RS36045 at 3538.552 kb on - strand, within RR42_RS36045 at 3538.552 kb on - strand, within RR42_RS36045 at 3538.571 kb on - strand, within RR42_RS36045 at 3538.571 kb on - strand, within RR42_RS36045 at 3538.599 kb on + strand, within RR42_RS36045 at 3538.600 kb on - strand, within RR42_RS36045 at 3538.602 kb on + strand, within RR42_RS36045 at 3538.647 kb on + strand, within RR42_RS36045 at 3538.667 kb on - strand, within RR42_RS36045 at 3538.793 kb on + strand at 3538.917 kb on + strand, within RR42_RS36050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,534,683 - RR42_RS36035 0.67 -0.2 3,534,708 - RR42_RS36035 0.68 -0.5 3,534,725 + RR42_RS36035 0.68 +0.4 3,534,752 + RR42_RS36035 0.70 +0.2 3,534,752 + RR42_RS36035 0.70 +2.4 3,534,752 + RR42_RS36035 0.70 +1.4 3,534,753 - RR42_RS36035 0.70 -0.5 3,534,753 - RR42_RS36035 0.70 +0.1 3,534,753 - RR42_RS36035 0.70 +0.1 3,534,761 + RR42_RS36035 0.70 -0.5 3,535,180 + RR42_RS36035 0.88 -0.6 3,535,181 - RR42_RS36035 0.88 +0.3 3,535,184 + RR42_RS36035 0.88 +0.3 3,535,184 + RR42_RS36035 0.88 +0.3 3,535,184 + RR42_RS36035 0.88 -0.6 3,535,184 + RR42_RS36035 0.88 +0.0 3,535,185 - RR42_RS36035 0.89 -1.7 3,535,223 + +0.8 3,535,223 + +0.1 3,535,224 - -0.9 3,535,224 - +0.0 3,535,226 + +0.0 3,535,226 + +0.1 3,535,226 + -0.4 3,535,226 + -0.2 3,535,227 - -1.4 3,535,304 + +0.4 3,535,364 + +0.3 3,535,365 - -0.9 3,535,367 + -0.7 3,535,367 + -0.7 3,535,368 - -1.0 3,535,368 - -0.5 3,535,368 - +1.0 3,535,368 - -0.2 3,535,368 - -0.3 3,535,391 + -1.0 3,535,392 - -0.0 3,535,500 + +0.4 3,535,500 + +0.1 3,535,500 + +0.5 3,535,500 + -0.3 3,535,500 + -0.9 3,535,500 + -0.7 3,535,500 + +0.4 3,535,500 + -0.9 3,535,501 - +0.4 3,535,501 - +0.8 3,535,501 - +0.7 3,535,501 - +0.2 3,535,501 - +0.2 3,535,501 - +0.1 3,535,543 + -0.6 3,535,556 + +0.4 3,535,556 + +1.0 3,535,556 + -0.5 3,535,556 + -0.2 3,535,556 + -1.6 3,535,556 + -0.3 3,535,556 + -0.9 3,535,557 - -0.3 3,535,557 - +1.7 3,535,557 - +0.8 3,535,557 - +0.6 3,535,557 - +0.8 3,535,557 - +0.1 3,535,557 - -2.9 3,535,557 - +0.7 3,535,557 - -1.1 3,535,557 - +0.3 3,535,557 - +1.0 3,535,557 - -0.9 3,535,575 - -0.1 3,536,009 - RR42_RS36040 0.16 -0.8 3,536,290 + RR42_RS36040 0.29 -0.7 3,536,291 - RR42_RS36040 0.29 -1.8 3,536,398 + RR42_RS36040 0.33 +0.5 3,536,398 + RR42_RS36040 0.33 +0.1 3,536,399 - RR42_RS36040 0.34 +1.4 3,536,401 + RR42_RS36040 0.34 +0.2 3,536,402 - RR42_RS36040 0.34 +0.5 3,536,402 - RR42_RS36040 0.34 +0.3 3,536,402 - RR42_RS36040 0.34 +0.6 3,536,443 + RR42_RS36040 0.35 +0.8 3,536,536 + RR42_RS36040 0.40 -0.4 3,536,536 + RR42_RS36040 0.40 +0.9 3,536,536 + RR42_RS36040 0.40 -0.4 3,536,536 + RR42_RS36040 0.40 +1.4 3,536,537 - RR42_RS36040 0.40 +0.2 3,536,540 - RR42_RS36040 0.40 -0.2 3,536,644 - RR42_RS36040 0.44 -0.6 3,536,665 + RR42_RS36040 0.45 +0.8 3,536,666 - RR42_RS36040 0.45 +0.4 3,536,666 - RR42_RS36040 0.45 +2.4 3,536,666 - RR42_RS36040 0.45 -0.2 3,536,704 + RR42_RS36040 0.47 +0.8 3,536,707 + RR42_RS36040 0.47 +1.7 3,536,707 + RR42_RS36040 0.47 -0.1 3,536,815 + RR42_RS36040 0.52 +1.1 3,536,863 + RR42_RS36040 0.54 +0.5 3,536,863 + RR42_RS36040 0.54 +0.8 3,536,864 - RR42_RS36040 0.54 -0.2 3,536,864 - RR42_RS36040 0.54 +1.7 3,536,864 - RR42_RS36040 0.54 +0.1 3,536,920 + RR42_RS36040 0.56 +1.0 3,536,938 + RR42_RS36040 0.57 -1.0 3,536,939 - RR42_RS36040 0.57 +0.4 3,537,026 - RR42_RS36040 0.61 -1.3 3,537,091 + RR42_RS36040 0.64 -0.8 3,537,169 + RR42_RS36040 0.67 +1.0 3,537,169 + RR42_RS36040 0.67 +0.3 3,537,187 + RR42_RS36040 0.68 +1.6 3,537,187 + RR42_RS36040 0.68 -0.8 3,537,187 + RR42_RS36040 0.68 +0.7 3,537,214 + RR42_RS36040 0.69 +0.5 3,537,540 + RR42_RS36040 0.83 +0.0 3,537,540 + RR42_RS36040 0.83 +0.8 3,537,540 + RR42_RS36040 0.83 +0.3 3,537,540 + RR42_RS36040 0.83 +0.7 3,537,541 - RR42_RS36040 0.83 +0.0 3,537,655 + RR42_RS36040 0.88 +0.8 3,537,694 + -0.6 3,537,697 + -1.5 3,537,697 + +0.3 3,537,698 - -1.2 3,537,698 - -0.2 3,537,835 + -1.8 3,537,835 + -0.4 3,537,851 - -0.5 3,537,913 + -1.0 3,538,387 - RR42_RS36045 0.41 +0.8 3,538,425 + RR42_RS36045 0.46 +2.2 3,538,426 - RR42_RS36045 0.46 +0.4 3,538,494 + RR42_RS36045 0.55 -1.7 3,538,494 + RR42_RS36045 0.55 +0.7 3,538,551 + RR42_RS36045 0.62 +0.5 3,538,552 - RR42_RS36045 0.62 -0.4 3,538,552 - RR42_RS36045 0.62 -0.3 3,538,571 - RR42_RS36045 0.65 -0.3 3,538,571 - RR42_RS36045 0.65 -0.2 3,538,599 + RR42_RS36045 0.68 +0.1 3,538,600 - RR42_RS36045 0.68 -0.3 3,538,602 + RR42_RS36045 0.68 -0.4 3,538,647 + RR42_RS36045 0.74 -2.2 3,538,667 - RR42_RS36045 0.77 -1.7 3,538,793 + +1.0 3,538,917 + RR42_RS36050 0.47 +0.5
Or see this region's nucleotide sequence