Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35645 and RR42_RS35650 are separated by 67 nucleotides RR42_RS35650 and RR42_RS35655 are separated by 120 nucleotides RR42_RS35655 and RR42_RS35660 are separated by 927 nucleotides
RR42_RS35645: RR42_RS35645 - MFS transporter, at 3,449,431 to 3,450,732
_RS35645
RR42_RS35650: RR42_RS35650 - hypothetical protein, at 3,450,800 to 3,451,204
_RS35650
RR42_RS35655: RR42_RS35655 - hypothetical protein, at 3,451,325 to 3,451,780
_RS35655
RR42_RS35660: RR42_RS35660 - hypothetical protein, at 3,452,708 to 3,453,019
_RS35660
Position (kb)
3451
3452 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3450.414 kb on - strand, within RR42_RS35645 at 3450.549 kb on + strand, within RR42_RS35645 at 3450.549 kb on + strand, within RR42_RS35645 at 3450.549 kb on + strand, within RR42_RS35645 at 3450.549 kb on + strand, within RR42_RS35645 at 3450.553 kb on + strand, within RR42_RS35645 at 3450.685 kb on + strand at 3450.685 kb on + strand at 3450.685 kb on + strand at 3450.685 kb on + strand at 3450.751 kb on + strand at 3450.752 kb on - strand at 3450.841 kb on + strand, within RR42_RS35650 at 3450.951 kb on + strand, within RR42_RS35650 at 3450.952 kb on - strand, within RR42_RS35650 at 3450.952 kb on - strand, within RR42_RS35650 at 3450.992 kb on + strand, within RR42_RS35650 at 3451.013 kb on + strand, within RR42_RS35650 at 3451.014 kb on - strand, within RR42_RS35650 at 3451.033 kb on + strand, within RR42_RS35650 at 3451.059 kb on + strand, within RR42_RS35650 at 3451.060 kb on - strand, within RR42_RS35650 at 3451.060 kb on - strand, within RR42_RS35650 at 3451.206 kb on + strand at 3451.206 kb on + strand at 3451.207 kb on - strand at 3451.210 kb on + strand at 3451.230 kb on + strand at 3451.231 kb on - strand at 3451.231 kb on - strand at 3451.231 kb on - strand at 3451.246 kb on + strand at 3451.246 kb on + strand at 3451.319 kb on + strand at 3451.425 kb on + strand, within RR42_RS35655 at 3451.425 kb on + strand, within RR42_RS35655 at 3451.426 kb on - strand, within RR42_RS35655 at 3451.566 kb on + strand, within RR42_RS35655 at 3451.566 kb on + strand, within RR42_RS35655 at 3451.566 kb on + strand, within RR42_RS35655 at 3451.571 kb on + strand, within RR42_RS35655 at 3451.571 kb on + strand, within RR42_RS35655 at 3451.571 kb on + strand, within RR42_RS35655 at 3451.571 kb on + strand, within RR42_RS35655 at 3451.572 kb on - strand, within RR42_RS35655 at 3451.572 kb on - strand, within RR42_RS35655 at 3451.592 kb on + strand, within RR42_RS35655 at 3451.593 kb on - strand, within RR42_RS35655 at 3451.670 kb on + strand, within RR42_RS35655 at 3451.670 kb on + strand, within RR42_RS35655 at 3451.670 kb on + strand, within RR42_RS35655 at 3451.788 kb on - strand at 3451.788 kb on - strand at 3451.788 kb on - strand at 3451.791 kb on - strand at 3451.791 kb on - strand at 3451.835 kb on - strand at 3452.030 kb on + strand at 3452.030 kb on + strand at 3452.030 kb on + strand at 3452.092 kb on - strand at 3452.209 kb on + strand at 3452.209 kb on + strand at 3452.370 kb on + strand at 3452.371 kb on - strand at 3452.431 kb on + strand at 3452.431 kb on + strand at 3452.445 kb on - strand at 3452.445 kb on - strand at 3452.584 kb on + strand at 3452.584 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.718 kb on + strand at 3452.719 kb on - strand at 3452.719 kb on - strand at 3452.733 kb on - strand at 3452.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,450,414 - RR42_RS35645 0.75 +0.4 3,450,549 + RR42_RS35645 0.86 +2.5 3,450,549 + RR42_RS35645 0.86 +2.4 3,450,549 + RR42_RS35645 0.86 +1.3 3,450,549 + RR42_RS35645 0.86 +1.8 3,450,553 + RR42_RS35645 0.86 +1.7 3,450,685 + +2.0 3,450,685 + +1.4 3,450,685 + -1.1 3,450,685 + +2.7 3,450,751 + +0.4 3,450,752 - -0.8 3,450,841 + RR42_RS35650 0.10 -1.7 3,450,951 + RR42_RS35650 0.37 +0.9 3,450,952 - RR42_RS35650 0.38 -0.4 3,450,952 - RR42_RS35650 0.38 -0.2 3,450,992 + RR42_RS35650 0.47 -1.3 3,451,013 + RR42_RS35650 0.53 +0.1 3,451,014 - RR42_RS35650 0.53 -2.0 3,451,033 + RR42_RS35650 0.58 +0.2 3,451,059 + RR42_RS35650 0.64 -2.6 3,451,060 - RR42_RS35650 0.64 -0.6 3,451,060 - RR42_RS35650 0.64 -1.2 3,451,206 + -0.5 3,451,206 + +0.0 3,451,207 - -1.0 3,451,210 + -0.7 3,451,230 + +0.3 3,451,231 - +0.7 3,451,231 - -0.9 3,451,231 - -0.4 3,451,246 + -0.1 3,451,246 + +0.1 3,451,319 + -0.3 3,451,425 + RR42_RS35655 0.22 +0.2 3,451,425 + RR42_RS35655 0.22 -0.0 3,451,426 - RR42_RS35655 0.22 +1.2 3,451,566 + RR42_RS35655 0.53 +0.7 3,451,566 + RR42_RS35655 0.53 +1.7 3,451,566 + RR42_RS35655 0.53 +0.8 3,451,571 + RR42_RS35655 0.54 -1.0 3,451,571 + RR42_RS35655 0.54 +0.3 3,451,571 + RR42_RS35655 0.54 -0.0 3,451,571 + RR42_RS35655 0.54 -0.1 3,451,572 - RR42_RS35655 0.54 -0.4 3,451,572 - RR42_RS35655 0.54 +0.5 3,451,592 + RR42_RS35655 0.59 +0.2 3,451,593 - RR42_RS35655 0.59 -0.8 3,451,670 + RR42_RS35655 0.76 -0.9 3,451,670 + RR42_RS35655 0.76 -0.8 3,451,670 + RR42_RS35655 0.76 -0.4 3,451,788 - +0.4 3,451,788 - +0.0 3,451,788 - -0.6 3,451,791 - +0.4 3,451,791 - -0.8 3,451,835 - -1.2 3,452,030 + -0.6 3,452,030 + -0.5 3,452,030 + -1.0 3,452,092 - +0.3 3,452,209 + +0.5 3,452,209 + -1.2 3,452,370 + +0.2 3,452,371 - +0.4 3,452,431 + -0.8 3,452,431 + +1.1 3,452,445 - +0.7 3,452,445 - +0.7 3,452,584 + +1.3 3,452,584 + +0.6 3,452,601 + -0.3 3,452,601 + +0.6 3,452,601 + -0.5 3,452,601 + -0.4 3,452,602 - +0.3 3,452,602 - +1.1 3,452,602 - -0.4 3,452,602 - -0.5 3,452,718 + -0.8 3,452,719 - -0.3 3,452,719 - +0.3 3,452,733 - +1.3 3,452,733 - +1.2
Or see this region's nucleotide sequence