Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35275 and RR42_RS35280 overlap by 4 nucleotides RR42_RS35280 and RR42_RS35285 are separated by 17 nucleotides
RR42_RS35275: RR42_RS35275 - membrane protein, at 3,368,843 to 3,369,814
_RS35275
RR42_RS35280: RR42_RS35280 - hypothetical protein, at 3,369,811 to 3,370,662
_RS35280
RR42_RS35285: RR42_RS35285 - hopanoid biosynthesis associated radical SAM protein HpnJ, at 3,370,680 to 3,372,101
_RS35285
Position (kb)
3369
3370
3371 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3368.844 kb on + strand at 3368.844 kb on + strand at 3368.845 kb on - strand at 3368.845 kb on - strand at 3368.845 kb on - strand at 3369.292 kb on + strand, within RR42_RS35275 at 3369.292 kb on + strand, within RR42_RS35275 at 3369.293 kb on - strand, within RR42_RS35275 at 3369.426 kb on + strand, within RR42_RS35275 at 3369.773 kb on + strand at 3369.798 kb on + strand at 3369.799 kb on - strand at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.993 kb on - strand, within RR42_RS35280 at 3369.993 kb on - strand, within RR42_RS35280 at 3370.083 kb on - strand, within RR42_RS35280 at 3370.801 kb on + strand at 3370.801 kb on + strand at 3370.802 kb on - strand at 3370.811 kb on + strand at 3370.811 kb on + strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.814 kb on - strand at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.952 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3371.243 kb on - strand, within RR42_RS35285 at 3371.243 kb on - strand, within RR42_RS35285 at 3371.499 kb on - strand, within RR42_RS35285 at 3371.500 kb on + strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.512 kb on + strand, within RR42_RS35285 at 3371.539 kb on + strand, within RR42_RS35285 at 3371.539 kb on + strand, within RR42_RS35285 at 3371.540 kb on - strand, within RR42_RS35285 at 3371.566 kb on + strand, within RR42_RS35285 at 3371.566 kb on + strand, within RR42_RS35285 at 3371.567 kb on - strand, within RR42_RS35285 at 3371.567 kb on - strand, within RR42_RS35285 at 3371.653 kb on + strand, within RR42_RS35285 at 3371.654 kb on - strand, within RR42_RS35285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,368,844 + +0.9 3,368,844 + -0.6 3,368,845 - +1.9 3,368,845 - -0.4 3,368,845 - -0.5 3,369,292 + RR42_RS35275 0.46 +0.1 3,369,292 + RR42_RS35275 0.46 -0.2 3,369,293 - RR42_RS35275 0.46 -0.2 3,369,426 + RR42_RS35275 0.60 -0.3 3,369,773 + -1.1 3,369,798 + -0.4 3,369,799 - -0.4 3,369,951 + RR42_RS35280 0.16 -0.4 3,369,951 + RR42_RS35280 0.16 -0.7 3,369,951 + RR42_RS35280 0.16 -0.4 3,369,951 + RR42_RS35280 0.16 -0.2 3,369,951 + RR42_RS35280 0.16 -0.9 3,369,951 + RR42_RS35280 0.16 -0.8 3,369,951 + RR42_RS35280 0.16 +0.4 3,369,952 - RR42_RS35280 0.17 -0.8 3,369,952 - RR42_RS35280 0.17 -0.5 3,369,952 - RR42_RS35280 0.17 -0.9 3,369,952 - RR42_RS35280 0.17 +0.2 3,369,952 - RR42_RS35280 0.17 -0.6 3,369,952 - RR42_RS35280 0.17 -0.5 3,369,952 - RR42_RS35280 0.17 -0.1 3,369,952 - RR42_RS35280 0.17 -1.2 3,369,992 + RR42_RS35280 0.21 -0.3 3,369,992 + RR42_RS35280 0.21 -0.3 3,369,992 + RR42_RS35280 0.21 -0.1 3,369,993 - RR42_RS35280 0.21 +0.8 3,369,993 - RR42_RS35280 0.21 -1.2 3,370,083 - RR42_RS35280 0.32 -0.7 3,370,801 + +0.7 3,370,801 + +0.3 3,370,802 - -0.1 3,370,811 + -1.3 3,370,811 + -0.6 3,370,812 - -0.3 3,370,812 - -0.9 3,370,812 - -0.1 3,370,812 - +0.7 3,370,812 - -0.3 3,370,813 + -0.8 3,370,813 + +0.1 3,370,813 + -2.0 3,370,813 + -1.6 3,370,813 + -0.6 3,370,813 + +0.2 3,370,813 + -1.4 3,370,814 - -0.4 3,370,834 + RR42_RS35285 0.11 -1.8 3,370,834 + RR42_RS35285 0.11 +0.6 3,370,834 + RR42_RS35285 0.11 -0.6 3,370,834 + RR42_RS35285 0.11 -0.8 3,370,834 + RR42_RS35285 0.11 -0.7 3,370,834 + RR42_RS35285 0.11 -0.5 3,370,834 + RR42_RS35285 0.11 -0.7 3,370,834 + RR42_RS35285 0.11 +0.1 3,370,835 - RR42_RS35285 0.11 -0.2 3,370,835 - RR42_RS35285 0.11 -1.2 3,370,835 - RR42_RS35285 0.11 +0.7 3,370,835 - RR42_RS35285 0.11 -0.2 3,370,835 - RR42_RS35285 0.11 -0.3 3,370,952 - RR42_RS35285 0.19 -0.6 3,370,954 - RR42_RS35285 0.19 +0.0 3,370,954 - RR42_RS35285 0.19 -0.2 3,370,954 - RR42_RS35285 0.19 -0.0 3,370,954 - RR42_RS35285 0.19 +1.4 3,371,243 - RR42_RS35285 0.40 -0.0 3,371,243 - RR42_RS35285 0.40 -0.5 3,371,499 - RR42_RS35285 0.58 -1.2 3,371,500 + RR42_RS35285 0.58 +0.1 3,371,501 - RR42_RS35285 0.58 -0.9 3,371,501 - RR42_RS35285 0.58 -1.6 3,371,501 - RR42_RS35285 0.58 -0.0 3,371,512 + RR42_RS35285 0.59 -0.8 3,371,539 + RR42_RS35285 0.60 -0.5 3,371,539 + RR42_RS35285 0.60 -0.2 3,371,540 - RR42_RS35285 0.60 -0.1 3,371,566 + RR42_RS35285 0.62 -1.3 3,371,566 + RR42_RS35285 0.62 +1.0 3,371,567 - RR42_RS35285 0.62 +1.7 3,371,567 - RR42_RS35285 0.62 +0.1 3,371,653 + RR42_RS35285 0.68 +0.1 3,371,654 - RR42_RS35285 0.68 +0.2
Or see this region's nucleotide sequence