Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS35105

Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS35100 and RR42_RS35105 are separated by 169 nucleotidesRR42_RS35105 and RR42_RS35110 are separated by 88 nucleotidesRR42_RS35110 and RR42_RS35115 are separated by 152 nucleotidesRR42_RS35115 and RR42_RS35120 are separated by 52 nucleotides RR42_RS35100: RR42_RS35100 - TetR family transcriptional regulator, at 3,327,552 to 3,328,160 _RS35100 RR42_RS35105: RR42_RS35105 - hydrolase, at 3,328,330 to 3,329,418 _RS35105 RR42_RS35110: RR42_RS35110 - hypothetical protein, at 3,329,507 to 3,329,689 _RS35110 RR42_RS35115: RR42_RS35115 - riboflavin synthase subunit beta, at 3,329,842 to 3,330,312 _RS35115 RR42_RS35120: RR42_RS35120 - benzoyl-CoA oxygenase, at 3,330,365 to 3,331,645 _RS35120 Position (kb) 3328 3329 3330Strain fitness (log2 ratio) -2 -1 0 1at 3327.339 kb on - strandat 3327.385 kb on + strandat 3327.386 kb on - strandat 3327.386 kb on - strandat 3327.386 kb on - strandat 3327.386 kb on - strandat 3327.407 kb on + strandat 3327.407 kb on + strandat 3327.407 kb on + strandat 3327.408 kb on - strandat 3327.408 kb on - strandat 3327.553 kb on + strandat 3327.931 kb on + strand, within RR42_RS35100at 3327.932 kb on - strand, within RR42_RS35100at 3327.966 kb on + strand, within RR42_RS35100at 3327.966 kb on + strand, within RR42_RS35100at 3327.978 kb on + strand, within RR42_RS35100at 3327.978 kb on + strand, within RR42_RS35100at 3327.978 kb on + strand, within RR42_RS35100at 3328.018 kb on + strand, within RR42_RS35100at 3328.019 kb on - strand, within RR42_RS35100at 3328.019 kb on - strand, within RR42_RS35100at 3328.019 kb on - strand, within RR42_RS35100at 3328.369 kb on + strandat 3328.370 kb on - strandat 3328.370 kb on - strandat 3328.370 kb on - strandat 3328.370 kb on - strandat 3328.599 kb on - strand, within RR42_RS35105at 3328.599 kb on - strand, within RR42_RS35105at 3328.599 kb on - strand, within RR42_RS35105at 3328.632 kb on + strand, within RR42_RS35105at 3328.633 kb on - strand, within RR42_RS35105at 3328.745 kb on - strand, within RR42_RS35105at 3328.745 kb on - strand, within RR42_RS35105at 3328.745 kb on - strand, within RR42_RS35105at 3328.962 kb on + strand, within RR42_RS35105at 3328.963 kb on - strand, within RR42_RS35105at 3329.101 kb on + strand, within RR42_RS35105at 3329.421 kb on + strandat 3329.515 kb on + strandat 3329.515 kb on + strandat 3329.515 kb on + strandat 3329.515 kb on + strandat 3329.516 kb on - strandat 3329.516 kb on - strandat 3329.516 kb on - strandat 3329.606 kb on + strand, within RR42_RS35110at 3329.606 kb on + strandat 3329.607 kb on - strand, within RR42_RS35110at 3329.689 kb on + strandat 3329.690 kb on - strandat 3330.094 kb on + strand, within RR42_RS35115at 3330.094 kb on + strand, within RR42_RS35115at 3330.095 kb on - strand, within RR42_RS35115at 3330.370 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml
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3,327,339 - +0.2
3,327,385 + -0.5
3,327,386 - -0.7
3,327,386 - +0.7
3,327,386 - +0.3
3,327,386 - +0.3
3,327,407 + +0.6
3,327,407 + +0.1
3,327,407 + -1.3
3,327,408 - +1.3
3,327,408 - -0.6
3,327,553 + -0.8
3,327,931 + RR42_RS35100 0.62 -0.1
3,327,932 - RR42_RS35100 0.62 -1.3
3,327,966 + RR42_RS35100 0.68 -0.4
3,327,966 + RR42_RS35100 0.68 +1.5
3,327,978 + RR42_RS35100 0.70 +1.0
3,327,978 + RR42_RS35100 0.70 +0.6
3,327,978 + RR42_RS35100 0.70 +0.4
3,328,018 + RR42_RS35100 0.77 +0.2
3,328,019 - RR42_RS35100 0.77 +0.9
3,328,019 - RR42_RS35100 0.77 +1.0
3,328,019 - RR42_RS35100 0.77 +0.4
3,328,369 + +0.8
3,328,370 - +0.1
3,328,370 - +0.1
3,328,370 - +0.5
3,328,370 - -0.0
3,328,599 - RR42_RS35105 0.25 +0.1
3,328,599 - RR42_RS35105 0.25 -2.0
3,328,599 - RR42_RS35105 0.25 +0.1
3,328,632 + RR42_RS35105 0.28 +0.1
3,328,633 - RR42_RS35105 0.28 -0.2
3,328,745 - RR42_RS35105 0.38 +0.1
3,328,745 - RR42_RS35105 0.38 -1.5
3,328,745 - RR42_RS35105 0.38 +0.1
3,328,962 + RR42_RS35105 0.58 -1.8
3,328,963 - RR42_RS35105 0.58 -0.2
3,329,101 + RR42_RS35105 0.71 +1.4
3,329,421 + +0.1
3,329,515 + -0.5
3,329,515 + -0.7
3,329,515 + +0.3
3,329,515 + +0.4
3,329,516 - +1.2
3,329,516 - -0.1
3,329,516 - +0.2
3,329,606 + RR42_RS35110 0.54 +0.2
3,329,606 + +0.1
3,329,607 - RR42_RS35110 0.55 +1.3
3,329,689 + +0.3
3,329,690 - -0.5
3,330,094 + RR42_RS35115 0.54 +0.5
3,330,094 + RR42_RS35115 0.54 +0.2
3,330,095 - RR42_RS35115 0.54 +0.2
3,330,370 - +0.6

Or see this region's nucleotide sequence