Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34905 and RR42_RS34910 are separated by 23 nucleotides RR42_RS34910 and RR42_RS34915 are separated by 65 nucleotides RR42_RS34915 and RR42_RS34920 are separated by 177 nucleotides
RR42_RS34905: RR42_RS34905 - enoyl-CoA hydratase, at 3,282,728 to 3,283,456
_RS34905
RR42_RS34910: RR42_RS34910 - cysteine dioxygenase, at 3,283,480 to 3,284,082
_RS34910
RR42_RS34915: RR42_RS34915 - carnitine dehydratase, at 3,284,148 to 3,285,335
_RS34915
RR42_RS34920: RR42_RS34920 - IclR family transcriptional regulator, at 3,285,513 to 3,286,319
_RS34920
Position (kb)
3284
3285
3286 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3283.347 kb on + strand, within RR42_RS34905 at 3283.348 kb on - strand, within RR42_RS34905 at 3283.348 kb on - strand, within RR42_RS34905 at 3283.466 kb on - strand at 3283.580 kb on + strand, within RR42_RS34910 at 3283.580 kb on + strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.594 kb on + strand, within RR42_RS34910 at 3283.616 kb on + strand, within RR42_RS34910 at 3283.892 kb on + strand, within RR42_RS34910 at 3283.892 kb on + strand, within RR42_RS34910 at 3283.893 kb on - strand, within RR42_RS34910 at 3283.893 kb on - strand, within RR42_RS34910 at 3283.910 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.914 kb on - strand, within RR42_RS34910 at 3284.149 kb on + strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.153 kb on + strand at 3284.154 kb on - strand at 3284.333 kb on + strand, within RR42_RS34915 at 3284.369 kb on - strand, within RR42_RS34915 at 3284.369 kb on - strand, within RR42_RS34915 at 3284.379 kb on - strand, within RR42_RS34915 at 3284.621 kb on - strand, within RR42_RS34915 at 3284.722 kb on + strand, within RR42_RS34915 at 3284.722 kb on + strand, within RR42_RS34915 at 3284.738 kb on + strand, within RR42_RS34915 at 3284.944 kb on + strand, within RR42_RS34915 at 3284.962 kb on + strand, within RR42_RS34915 at 3284.962 kb on + strand, within RR42_RS34915 at 3284.963 kb on - strand, within RR42_RS34915 at 3284.963 kb on - strand, within RR42_RS34915 at 3285.422 kb on - strand at 3285.423 kb on + strand at 3285.438 kb on + strand at 3285.439 kb on - strand at 3285.444 kb on - strand at 3285.504 kb on + strand at 3285.504 kb on + strand at 3285.504 kb on + strand at 3285.505 kb on - strand at 3285.660 kb on + strand, within RR42_RS34920 at 3285.660 kb on + strand, within RR42_RS34920 at 3285.660 kb on + strand, within RR42_RS34920 at 3285.660 kb on + strand, within RR42_RS34920 at 3285.661 kb on - strand, within RR42_RS34920 at 3285.661 kb on - strand, within RR42_RS34920 at 3285.661 kb on - strand, within RR42_RS34920 at 3285.661 kb on - strand, within RR42_RS34920 at 3285.661 kb on - strand, within RR42_RS34920 at 3285.847 kb on - strand, within RR42_RS34920 at 3285.847 kb on - strand, within RR42_RS34920 at 3285.847 kb on - strand, within RR42_RS34920 at 3285.886 kb on - strand, within RR42_RS34920 at 3286.066 kb on - strand, within RR42_RS34920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,283,347 + RR42_RS34905 0.85 +0.4 3,283,348 - RR42_RS34905 0.85 -0.4 3,283,348 - RR42_RS34905 0.85 +0.4 3,283,466 - +0.4 3,283,580 + RR42_RS34910 0.17 -0.9 3,283,580 + RR42_RS34910 0.17 -1.2 3,283,581 - RR42_RS34910 0.17 -0.8 3,283,581 - RR42_RS34910 0.17 +0.4 3,283,581 - RR42_RS34910 0.17 +0.0 3,283,594 + RR42_RS34910 0.19 -0.8 3,283,616 + RR42_RS34910 0.23 -0.6 3,283,892 + RR42_RS34910 0.68 -0.2 3,283,892 + RR42_RS34910 0.68 -0.2 3,283,893 - RR42_RS34910 0.68 -2.0 3,283,893 - RR42_RS34910 0.68 +0.5 3,283,910 + RR42_RS34910 0.71 -0.8 3,283,913 + RR42_RS34910 0.72 +0.0 3,283,913 + RR42_RS34910 0.72 +1.1 3,283,913 + RR42_RS34910 0.72 +0.3 3,283,914 - RR42_RS34910 0.72 -1.9 3,284,149 + +0.0 3,284,150 - +1.3 3,284,150 - -0.3 3,284,150 - -0.3 3,284,150 - +0.3 3,284,150 - -0.0 3,284,153 + +2.0 3,284,154 - +0.4 3,284,333 + RR42_RS34915 0.16 -0.7 3,284,369 - RR42_RS34915 0.19 -0.1 3,284,369 - RR42_RS34915 0.19 +1.4 3,284,379 - RR42_RS34915 0.19 -0.7 3,284,621 - RR42_RS34915 0.40 -0.5 3,284,722 + RR42_RS34915 0.48 +1.3 3,284,722 + RR42_RS34915 0.48 -0.4 3,284,738 + RR42_RS34915 0.50 -0.5 3,284,944 + RR42_RS34915 0.67 -1.7 3,284,962 + RR42_RS34915 0.69 -0.3 3,284,962 + RR42_RS34915 0.69 -1.0 3,284,963 - RR42_RS34915 0.69 -0.8 3,284,963 - RR42_RS34915 0.69 -1.1 3,285,422 - -1.7 3,285,423 + -0.4 3,285,438 + -2.0 3,285,439 - -0.2 3,285,444 - +0.2 3,285,504 + -0.9 3,285,504 + +0.7 3,285,504 + -1.3 3,285,505 - -1.4 3,285,660 + RR42_RS34920 0.18 +0.4 3,285,660 + RR42_RS34920 0.18 -0.6 3,285,660 + RR42_RS34920 0.18 -0.4 3,285,660 + RR42_RS34920 0.18 +0.1 3,285,661 - RR42_RS34920 0.18 -0.3 3,285,661 - RR42_RS34920 0.18 -0.0 3,285,661 - RR42_RS34920 0.18 -0.1 3,285,661 - RR42_RS34920 0.18 +0.1 3,285,661 - RR42_RS34920 0.18 +1.5 3,285,847 - RR42_RS34920 0.41 +0.1 3,285,847 - RR42_RS34920 0.41 +0.1 3,285,847 - RR42_RS34920 0.41 -0.2 3,285,886 - RR42_RS34920 0.46 +0.2 3,286,066 - RR42_RS34920 0.69 -0.4
Or see this region's nucleotide sequence