Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34460 and RR42_RS34465 are separated by 5 nucleotides RR42_RS34465 and RR42_RS34470 are separated by 65 nucleotides
RR42_RS34460: RR42_RS34460 - GDP-L-fucose synthase, at 3,160,524 to 3,161,513
_RS34460
RR42_RS34465: RR42_RS34465 - GDP-mannose 4,6-dehydratase, at 3,161,519 to 3,162,637
_RS34465
RR42_RS34470: RR42_RS34470 - protein tyrosine kinase, at 3,162,703 to 3,164,937
_RS34470
Position (kb)
3161
3162
3163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3160.613 kb on - strand at 3160.613 kb on - strand at 3160.812 kb on + strand, within RR42_RS34460 at 3160.993 kb on + strand, within RR42_RS34460 at 3160.994 kb on - strand, within RR42_RS34460 at 3161.039 kb on - strand, within RR42_RS34460 at 3161.063 kb on - strand, within RR42_RS34460 at 3161.105 kb on - strand, within RR42_RS34460 at 3161.179 kb on + strand, within RR42_RS34460 at 3161.179 kb on + strand, within RR42_RS34460 at 3161.180 kb on - strand, within RR42_RS34460 at 3161.180 kb on - strand, within RR42_RS34460 at 3161.227 kb on + strand, within RR42_RS34460 at 3161.489 kb on + strand at 3161.520 kb on + strand at 3161.520 kb on + strand at 3161.521 kb on - strand at 3161.865 kb on + strand, within RR42_RS34465 at 3161.865 kb on + strand, within RR42_RS34465 at 3161.866 kb on - strand, within RR42_RS34465 at 3161.986 kb on - strand, within RR42_RS34465 at 3161.986 kb on - strand, within RR42_RS34465 at 3162.102 kb on + strand, within RR42_RS34465 at 3162.103 kb on - strand, within RR42_RS34465 at 3162.123 kb on + strand, within RR42_RS34465 at 3162.147 kb on + strand, within RR42_RS34465 at 3162.250 kb on - strand, within RR42_RS34465 at 3162.384 kb on + strand, within RR42_RS34465 at 3162.385 kb on - strand, within RR42_RS34465 at 3162.385 kb on - strand, within RR42_RS34465 at 3162.514 kb on + strand, within RR42_RS34465 at 3162.585 kb on + strand at 3162.585 kb on + strand at 3162.585 kb on + strand at 3162.585 kb on + strand at 3162.586 kb on - strand at 3162.663 kb on + strand at 3162.704 kb on + strand at 3162.704 kb on + strand at 3162.959 kb on + strand, within RR42_RS34470 at 3163.508 kb on + strand, within RR42_RS34470
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,160,613 - +0.0 3,160,613 - +1.9 3,160,812 + RR42_RS34460 0.29 +1.8 3,160,993 + RR42_RS34460 0.47 -1.7 3,160,994 - RR42_RS34460 0.47 -0.0 3,161,039 - RR42_RS34460 0.52 -0.5 3,161,063 - RR42_RS34460 0.54 -0.5 3,161,105 - RR42_RS34460 0.59 -0.4 3,161,179 + RR42_RS34460 0.66 -0.9 3,161,179 + RR42_RS34460 0.66 -1.1 3,161,180 - RR42_RS34460 0.66 -0.6 3,161,180 - RR42_RS34460 0.66 +0.6 3,161,227 + RR42_RS34460 0.71 -0.1 3,161,489 + +0.7 3,161,520 + -1.7 3,161,520 + +0.1 3,161,521 - -1.1 3,161,865 + RR42_RS34465 0.31 +0.6 3,161,865 + RR42_RS34465 0.31 +0.6 3,161,866 - RR42_RS34465 0.31 +0.2 3,161,986 - RR42_RS34465 0.42 -1.5 3,161,986 - RR42_RS34465 0.42 -2.5 3,162,102 + RR42_RS34465 0.52 -0.0 3,162,103 - RR42_RS34465 0.52 -0.6 3,162,123 + RR42_RS34465 0.54 -1.5 3,162,147 + RR42_RS34465 0.56 +0.4 3,162,250 - RR42_RS34465 0.65 -1.3 3,162,384 + RR42_RS34465 0.77 -1.0 3,162,385 - RR42_RS34465 0.77 +0.8 3,162,385 - RR42_RS34465 0.77 -1.9 3,162,514 + RR42_RS34465 0.89 -0.6 3,162,585 + +0.1 3,162,585 + +0.6 3,162,585 + -0.5 3,162,585 + +0.3 3,162,586 - -0.2 3,162,663 + -0.7 3,162,704 + -0.7 3,162,704 + -0.8 3,162,959 + RR42_RS34470 0.11 -0.4 3,163,508 + RR42_RS34470 0.36 +0.3
Or see this region's nucleotide sequence