Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33100 and RR42_RS33105 are separated by 133 nucleotides RR42_RS33105 and RR42_RS33110 are separated by 250 nucleotides RR42_RS33110 and RR42_RS33115 are separated by 9 nucleotides
RR42_RS33100: RR42_RS33100 - oxidoreductase, at 2,833,749 to 2,834,609
_RS33100
RR42_RS33105: RR42_RS33105 - FAD-dependent oxidoreductase, at 2,834,743 to 2,836,026
_RS33105
RR42_RS33110: RR42_RS33110 - hypothetical protein, at 2,836,277 to 2,836,564
_RS33110
RR42_RS33115: RR42_RS33115 - hypothetical protein, at 2,836,574 to 2,837,602
_RS33115
Position (kb)
2834
2835
2836
2837 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2833.754 kb on + strand at 2833.771 kb on - strand at 2833.771 kb on - strand at 2833.800 kb on - strand at 2833.911 kb on - strand, within RR42_RS33100 at 2833.911 kb on - strand, within RR42_RS33100 at 2833.930 kb on - strand, within RR42_RS33100 at 2834.128 kb on + strand, within RR42_RS33100 at 2834.128 kb on + strand, within RR42_RS33100 at 2834.128 kb on + strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.129 kb on - strand, within RR42_RS33100 at 2834.147 kb on + strand, within RR42_RS33100 at 2834.147 kb on + strand, within RR42_RS33100 at 2834.147 kb on + strand, within RR42_RS33100 at 2834.148 kb on - strand, within RR42_RS33100 at 2834.367 kb on + strand, within RR42_RS33100 at 2834.493 kb on - strand, within RR42_RS33100 at 2834.504 kb on + strand, within RR42_RS33100 at 2834.505 kb on - strand, within RR42_RS33100 at 2834.567 kb on - strand at 2834.628 kb on + strand at 2834.693 kb on - strand at 2834.867 kb on + strand at 2834.867 kb on + strand at 2834.867 kb on + strand at 2834.867 kb on + strand at 2834.868 kb on - strand at 2834.868 kb on - strand at 2834.868 kb on - strand at 2835.089 kb on + strand, within RR42_RS33105 at 2835.089 kb on + strand, within RR42_RS33105 at 2835.090 kb on - strand, within RR42_RS33105 at 2835.119 kb on + strand, within RR42_RS33105 at 2835.119 kb on + strand, within RR42_RS33105 at 2835.119 kb on + strand, within RR42_RS33105 at 2835.120 kb on - strand, within RR42_RS33105 at 2835.120 kb on - strand, within RR42_RS33105 at 2835.272 kb on + strand, within RR42_RS33105 at 2835.272 kb on + strand, within RR42_RS33105 at 2835.273 kb on - strand, within RR42_RS33105 at 2835.273 kb on - strand, within RR42_RS33105 at 2835.273 kb on - strand, within RR42_RS33105 at 2835.332 kb on + strand, within RR42_RS33105 at 2835.332 kb on + strand, within RR42_RS33105 at 2835.332 kb on + strand, within RR42_RS33105 at 2835.333 kb on - strand, within RR42_RS33105 at 2835.333 kb on - strand, within RR42_RS33105 at 2835.333 kb on - strand, within RR42_RS33105 at 2835.335 kb on + strand, within RR42_RS33105 at 2835.335 kb on + strand, within RR42_RS33105 at 2835.335 kb on + strand, within RR42_RS33105 at 2835.376 kb on + strand, within RR42_RS33105 at 2835.378 kb on - strand, within RR42_RS33105 at 2835.521 kb on + strand, within RR42_RS33105 at 2835.656 kb on + strand, within RR42_RS33105 at 2835.657 kb on - strand, within RR42_RS33105 at 2835.870 kb on + strand, within RR42_RS33105 at 2835.870 kb on + strand, within RR42_RS33105 at 2835.929 kb on + strand at 2835.929 kb on + strand at 2835.929 kb on + strand at 2835.929 kb on + strand at 2835.930 kb on - strand at 2835.930 kb on - strand at 2835.930 kb on - strand at 2835.930 kb on - strand at 2835.977 kb on + strand at 2835.978 kb on - strand at 2835.978 kb on - strand at 2836.279 kb on - strand at 2836.279 kb on - strand at 2836.383 kb on + strand, within RR42_RS33110 at 2836.389 kb on + strand, within RR42_RS33110 at 2836.389 kb on + strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.390 kb on - strand, within RR42_RS33110 at 2836.461 kb on + strand, within RR42_RS33110 at 2836.541 kb on + strand at 2836.541 kb on + strand at 2836.541 kb on + strand at 2836.542 kb on - strand at 2836.542 kb on - strand at 2836.542 kb on - strand at 2836.542 kb on - strand at 2836.797 kb on + strand, within RR42_RS33115 at 2836.797 kb on + strand, within RR42_RS33115 at 2836.798 kb on - strand, within RR42_RS33115
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,833,754 + -0.2 2,833,771 - +0.7 2,833,771 - -0.5 2,833,800 - +0.3 2,833,911 - RR42_RS33100 0.19 +0.6 2,833,911 - RR42_RS33100 0.19 -0.7 2,833,930 - RR42_RS33100 0.21 -1.1 2,834,128 + RR42_RS33100 0.44 +1.0 2,834,128 + RR42_RS33100 0.44 +0.5 2,834,128 + RR42_RS33100 0.44 +1.1 2,834,129 - RR42_RS33100 0.44 -0.4 2,834,129 - RR42_RS33100 0.44 +0.1 2,834,129 - RR42_RS33100 0.44 -0.4 2,834,129 - RR42_RS33100 0.44 -0.2 2,834,129 - RR42_RS33100 0.44 -0.4 2,834,129 - RR42_RS33100 0.44 +0.2 2,834,147 + RR42_RS33100 0.46 -1.3 2,834,147 + RR42_RS33100 0.46 -0.2 2,834,147 + RR42_RS33100 0.46 -4.4 2,834,148 - RR42_RS33100 0.46 -0.2 2,834,367 + RR42_RS33100 0.72 -0.1 2,834,493 - RR42_RS33100 0.86 +0.5 2,834,504 + RR42_RS33100 0.88 +0.3 2,834,505 - RR42_RS33100 0.88 -0.3 2,834,567 - +0.2 2,834,628 + -0.6 2,834,693 - -0.1 2,834,867 + -0.4 2,834,867 + -0.2 2,834,867 + +0.2 2,834,867 + +0.5 2,834,868 - +0.6 2,834,868 - +2.5 2,834,868 - -1.0 2,835,089 + RR42_RS33105 0.27 -0.8 2,835,089 + RR42_RS33105 0.27 +0.1 2,835,090 - RR42_RS33105 0.27 +0.5 2,835,119 + RR42_RS33105 0.29 +0.4 2,835,119 + RR42_RS33105 0.29 -0.6 2,835,119 + RR42_RS33105 0.29 -0.5 2,835,120 - RR42_RS33105 0.29 -0.4 2,835,120 - RR42_RS33105 0.29 +1.7 2,835,272 + RR42_RS33105 0.41 -0.0 2,835,272 + RR42_RS33105 0.41 +0.0 2,835,273 - RR42_RS33105 0.41 +0.1 2,835,273 - RR42_RS33105 0.41 +0.3 2,835,273 - RR42_RS33105 0.41 -0.1 2,835,332 + RR42_RS33105 0.46 +0.3 2,835,332 + RR42_RS33105 0.46 -0.9 2,835,332 + RR42_RS33105 0.46 -1.9 2,835,333 - RR42_RS33105 0.46 +1.0 2,835,333 - RR42_RS33105 0.46 +0.4 2,835,333 - RR42_RS33105 0.46 +0.9 2,835,335 + RR42_RS33105 0.46 -0.0 2,835,335 + RR42_RS33105 0.46 -0.2 2,835,335 + RR42_RS33105 0.46 +0.2 2,835,376 + RR42_RS33105 0.49 -0.4 2,835,378 - RR42_RS33105 0.49 +0.6 2,835,521 + RR42_RS33105 0.61 +0.6 2,835,656 + RR42_RS33105 0.71 +0.3 2,835,657 - RR42_RS33105 0.71 -0.5 2,835,870 + RR42_RS33105 0.88 -0.2 2,835,870 + RR42_RS33105 0.88 +1.2 2,835,929 + +0.2 2,835,929 + -1.6 2,835,929 + +0.8 2,835,929 + +1.1 2,835,930 - +0.7 2,835,930 - -0.3 2,835,930 - -1.8 2,835,930 - -0.8 2,835,977 + +0.1 2,835,978 - -0.3 2,835,978 - +1.0 2,836,279 - -0.3 2,836,279 - -1.1 2,836,383 + RR42_RS33110 0.37 +0.4 2,836,389 + RR42_RS33110 0.39 +0.1 2,836,389 + RR42_RS33110 0.39 -0.4 2,836,390 - RR42_RS33110 0.39 -0.5 2,836,390 - RR42_RS33110 0.39 +0.5 2,836,390 - RR42_RS33110 0.39 +0.8 2,836,390 - RR42_RS33110 0.39 +1.5 2,836,390 - RR42_RS33110 0.39 -0.5 2,836,390 - RR42_RS33110 0.39 +0.2 2,836,461 + RR42_RS33110 0.64 +0.0 2,836,541 + -0.8 2,836,541 + -0.1 2,836,541 + -0.6 2,836,542 - +0.7 2,836,542 - -0.9 2,836,542 - +0.3 2,836,542 - +1.1 2,836,797 + RR42_RS33115 0.22 +1.3 2,836,797 + RR42_RS33115 0.22 -0.8 2,836,798 - RR42_RS33115 0.22 -1.1
Or see this region's nucleotide sequence