Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32675 and RR42_RS32680 are separated by 84 nucleotides RR42_RS32680 and RR42_RS32685 are separated by 10 nucleotides RR42_RS32685 and RR42_RS32690 are separated by 77 nucleotides RR42_RS32690 and RR42_RS32695 are separated by 409 nucleotides
RR42_RS32675: RR42_RS32675 - phenol 2-monooxygenase, at 2,725,847 to 2,727,361
_RS32675
RR42_RS32680: RR42_RS32680 - monooxygenase, at 2,727,446 to 2,727,718
_RS32680
RR42_RS32685: RR42_RS32685 - phenol hydroxylase, at 2,727,729 to 2,728,724
_RS32685
RR42_RS32690: RR42_RS32690 - phenol hydroxylase, at 2,728,802 to 2,729,020
_RS32690
RR42_RS32695: RR42_RS32695 - Fis family transcriptional regulator, at 2,729,430 to 2,731,145
_RS32695
Position (kb)
2727
2728
2729 Strain fitness (log2 ratio)
-1
0
1 at 2726.766 kb on + strand, within RR42_RS32675 at 2726.854 kb on - strand, within RR42_RS32675 at 2726.872 kb on + strand, within RR42_RS32675 at 2726.872 kb on + strand, within RR42_RS32675 at 2726.872 kb on + strand, within RR42_RS32675 at 2726.873 kb on - strand, within RR42_RS32675 at 2726.873 kb on - strand, within RR42_RS32675 at 2726.901 kb on + strand, within RR42_RS32675 at 2726.901 kb on + strand, within RR42_RS32675 at 2727.021 kb on + strand, within RR42_RS32675 at 2727.175 kb on - strand, within RR42_RS32675 at 2727.175 kb on - strand, within RR42_RS32675 at 2727.249 kb on + strand at 2727.250 kb on - strand at 2727.250 kb on - strand at 2727.285 kb on + strand at 2727.285 kb on + strand at 2727.324 kb on + strand at 2727.418 kb on - strand at 2727.700 kb on + strand at 2728.229 kb on - strand, within RR42_RS32685 at 2728.261 kb on + strand, within RR42_RS32685 at 2728.474 kb on + strand, within RR42_RS32685 at 2728.489 kb on + strand, within RR42_RS32685 at 2728.552 kb on + strand, within RR42_RS32685 at 2728.615 kb on + strand, within RR42_RS32685 at 2728.616 kb on - strand, within RR42_RS32685 at 2728.676 kb on - strand at 2728.753 kb on + strand at 2728.754 kb on - strand at 2728.754 kb on - strand at 2729.045 kb on + strand at 2729.045 kb on + strand at 2729.054 kb on + strand at 2729.055 kb on - strand at 2729.353 kb on + strand at 2729.353 kb on + strand at 2729.354 kb on - strand at 2729.394 kb on - strand at 2729.522 kb on + strand at 2729.522 kb on + strand at 2729.523 kb on - strand at 2729.600 kb on + strand at 2729.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,726,766 + RR42_RS32675 0.61 +0.1 2,726,854 - RR42_RS32675 0.66 -1.1 2,726,872 + RR42_RS32675 0.68 +0.3 2,726,872 + RR42_RS32675 0.68 -0.6 2,726,872 + RR42_RS32675 0.68 +1.4 2,726,873 - RR42_RS32675 0.68 -0.4 2,726,873 - RR42_RS32675 0.68 +1.0 2,726,901 + RR42_RS32675 0.70 +0.6 2,726,901 + RR42_RS32675 0.70 -0.6 2,727,021 + RR42_RS32675 0.77 -0.1 2,727,175 - RR42_RS32675 0.88 -0.9 2,727,175 - RR42_RS32675 0.88 +1.0 2,727,249 + -0.2 2,727,250 - +1.2 2,727,250 - -0.5 2,727,285 + +0.2 2,727,285 + -1.2 2,727,324 + -0.3 2,727,418 - +0.9 2,727,700 + -0.3 2,728,229 - RR42_RS32685 0.50 +0.7 2,728,261 + RR42_RS32685 0.53 -0.2 2,728,474 + RR42_RS32685 0.75 -1.5 2,728,489 + RR42_RS32685 0.76 -0.4 2,728,552 + RR42_RS32685 0.83 -0.8 2,728,615 + RR42_RS32685 0.89 +0.2 2,728,616 - RR42_RS32685 0.89 -1.3 2,728,676 - -0.5 2,728,753 + +0.2 2,728,754 - +0.0 2,728,754 - -0.1 2,729,045 + -0.5 2,729,045 + -1.3 2,729,054 + +1.0 2,729,055 - -0.2 2,729,353 + -0.3 2,729,353 + +0.2 2,729,354 - +0.8 2,729,394 - -0.3 2,729,522 + -0.3 2,729,522 + +0.7 2,729,523 - -0.0 2,729,600 + +0.4 2,729,601 - -0.5
Or see this region's nucleotide sequence