Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32345 and RR42_RS32350 overlap by 1 nucleotides RR42_RS32350 and RR42_RS32355 are separated by 127 nucleotides RR42_RS32355 and RR42_RS32360 are separated by 601 nucleotides
RR42_RS32345: RR42_RS32345 - hypothetical protein, at 2,649,965 to 2,650,381
_RS32345
RR42_RS32350: RR42_RS32350 - integral membrane protein, at 2,650,381 to 2,651,067
_RS32350
RR42_RS32355: RR42_RS32355 - polysaccharide deacetylase, at 2,651,195 to 2,652,058
_RS32355
RR42_RS32360: RR42_RS32360 - hypothetical protein, at 2,652,660 to 2,653,226
_RS32360
Position (kb)
2651
2652
2653 Strain fitness (log2 ratio)
-2
-1
0
1 at 2650.532 kb on + strand, within RR42_RS32350 at 2650.609 kb on - strand, within RR42_RS32350 at 2650.775 kb on + strand, within RR42_RS32350 at 2650.776 kb on - strand, within RR42_RS32350 at 2651.126 kb on + strand at 2651.127 kb on - strand at 2651.127 kb on - strand at 2651.127 kb on - strand at 2651.131 kb on + strand at 2651.131 kb on + strand at 2651.132 kb on - strand at 2651.217 kb on + strand at 2651.218 kb on - strand at 2651.337 kb on + strand, within RR42_RS32355 at 2651.338 kb on - strand, within RR42_RS32355 at 2651.362 kb on - strand, within RR42_RS32355 at 2651.461 kb on + strand, within RR42_RS32355 at 2651.468 kb on - strand, within RR42_RS32355 at 2651.468 kb on - strand, within RR42_RS32355 at 2651.468 kb on - strand, within RR42_RS32355 at 2651.468 kb on - strand, within RR42_RS32355 at 2651.492 kb on - strand, within RR42_RS32355 at 2651.502 kb on + strand, within RR42_RS32355 at 2651.562 kb on + strand, within RR42_RS32355 at 2651.587 kb on - strand, within RR42_RS32355 at 2651.695 kb on + strand, within RR42_RS32355 at 2651.695 kb on + strand, within RR42_RS32355 at 2652.377 kb on - strand at 2652.757 kb on + strand, within RR42_RS32360 at 2652.792 kb on - strand, within RR42_RS32360 at 2652.792 kb on - strand, within RR42_RS32360 at 2652.968 kb on - strand, within RR42_RS32360 at 2653.014 kb on - strand, within RR42_RS32360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,650,532 + RR42_RS32350 0.22 -1.8 2,650,609 - RR42_RS32350 0.33 +0.5 2,650,775 + RR42_RS32350 0.57 -0.7 2,650,776 - RR42_RS32350 0.57 +0.2 2,651,126 + +1.0 2,651,127 - -0.1 2,651,127 - -0.7 2,651,127 - +0.4 2,651,131 + -0.2 2,651,131 + +1.6 2,651,132 - -0.1 2,651,217 + +0.8 2,651,218 - -0.5 2,651,337 + RR42_RS32355 0.16 -0.7 2,651,338 - RR42_RS32355 0.17 +0.4 2,651,362 - RR42_RS32355 0.19 -0.2 2,651,461 + RR42_RS32355 0.31 +0.1 2,651,468 - RR42_RS32355 0.32 -1.0 2,651,468 - RR42_RS32355 0.32 +0.2 2,651,468 - RR42_RS32355 0.32 -2.1 2,651,468 - RR42_RS32355 0.32 -2.3 2,651,492 - RR42_RS32355 0.34 -1.2 2,651,502 + RR42_RS32355 0.36 -0.6 2,651,562 + RR42_RS32355 0.42 +0.3 2,651,587 - RR42_RS32355 0.45 +0.3 2,651,695 + RR42_RS32355 0.58 -0.9 2,651,695 + RR42_RS32355 0.58 -0.5 2,652,377 - -1.5 2,652,757 + RR42_RS32360 0.17 +0.3 2,652,792 - RR42_RS32360 0.23 +0.6 2,652,792 - RR42_RS32360 0.23 -0.5 2,652,968 - RR42_RS32360 0.54 -0.4 2,653,014 - RR42_RS32360 0.62 +0.9
Or see this region's nucleotide sequence