Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32210 and RR42_RS32215 are separated by 425 nucleotides RR42_RS32215 and RR42_RS32220 are separated by 71 nucleotides
RR42_RS32210: RR42_RS32210 - AraC family transcriptional regulator, at 2,612,914 to 2,613,699
_RS32210
RR42_RS32215: RR42_RS32215 - aminomethyltransferase, at 2,614,125 to 2,615,000
_RS32215
RR42_RS32220: RR42_RS32220 - glycosyl transferase family 2, at 2,615,072 to 2,616,211
_RS32220
Position (kb)
2614
2615
2616 Strain fitness (log2 ratio)
-2
-1
0
1 at 2613.380 kb on - strand, within RR42_RS32210 at 2613.486 kb on + strand, within RR42_RS32210 at 2613.567 kb on + strand, within RR42_RS32210 at 2613.568 kb on - strand, within RR42_RS32210 at 2613.606 kb on + strand, within RR42_RS32210 at 2613.607 kb on - strand, within RR42_RS32210 at 2613.740 kb on + strand at 2613.740 kb on + strand at 2613.821 kb on + strand at 2613.821 kb on + strand at 2613.821 kb on + strand at 2613.821 kb on + strand at 2613.822 kb on - strand at 2613.822 kb on - strand at 2613.822 kb on - strand at 2613.822 kb on - strand at 2613.860 kb on + strand at 2613.863 kb on + strand at 2613.864 kb on - strand at 2613.900 kb on - strand at 2613.939 kb on + strand at 2613.940 kb on - strand at 2613.993 kb on + strand at 2614.220 kb on + strand, within RR42_RS32215 at 2614.220 kb on + strand, within RR42_RS32215 at 2614.221 kb on - strand, within RR42_RS32215 at 2614.221 kb on - strand, within RR42_RS32215 at 2614.245 kb on + strand, within RR42_RS32215 at 2614.471 kb on - strand, within RR42_RS32215 at 2614.471 kb on - strand, within RR42_RS32215 at 2614.471 kb on - strand, within RR42_RS32215 at 2614.545 kb on + strand, within RR42_RS32215 at 2614.563 kb on - strand, within RR42_RS32215 at 2614.698 kb on + strand, within RR42_RS32215 at 2614.699 kb on - strand, within RR42_RS32215 at 2614.699 kb on - strand, within RR42_RS32215 at 2614.734 kb on + strand, within RR42_RS32215 at 2614.734 kb on + strand, within RR42_RS32215 at 2614.735 kb on - strand, within RR42_RS32215 at 2615.028 kb on + strand at 2615.028 kb on + strand at 2615.044 kb on + strand at 2615.044 kb on + strand at 2615.302 kb on + strand, within RR42_RS32220 at 2615.302 kb on + strand, within RR42_RS32220 at 2615.302 kb on + strand, within RR42_RS32220 at 2615.303 kb on - strand, within RR42_RS32220 at 2615.303 kb on - strand, within RR42_RS32220 at 2615.475 kb on + strand, within RR42_RS32220 at 2615.779 kb on + strand, within RR42_RS32220 at 2615.780 kb on - strand, within RR42_RS32220 at 2615.901 kb on - strand, within RR42_RS32220 at 2615.916 kb on - strand, within RR42_RS32220 at 2615.916 kb on - strand, within RR42_RS32220 at 2615.964 kb on - strand, within RR42_RS32220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,613,380 - RR42_RS32210 0.59 +0.4 2,613,486 + RR42_RS32210 0.73 -1.3 2,613,567 + RR42_RS32210 0.83 -0.0 2,613,568 - RR42_RS32210 0.83 +0.4 2,613,606 + RR42_RS32210 0.88 +0.1 2,613,607 - RR42_RS32210 0.88 +0.6 2,613,740 + -1.0 2,613,740 + +0.2 2,613,821 + -1.6 2,613,821 + -0.1 2,613,821 + -1.1 2,613,821 + +1.3 2,613,822 - -0.7 2,613,822 - -0.5 2,613,822 - -0.4 2,613,822 - +0.8 2,613,860 + -0.0 2,613,863 + +0.4 2,613,864 - -0.3 2,613,900 - -0.0 2,613,939 + -2.1 2,613,940 - +1.0 2,613,993 + -0.0 2,614,220 + RR42_RS32215 0.11 -0.7 2,614,220 + RR42_RS32215 0.11 -0.1 2,614,221 - RR42_RS32215 0.11 -0.1 2,614,221 - RR42_RS32215 0.11 -0.2 2,614,245 + RR42_RS32215 0.14 -0.5 2,614,471 - RR42_RS32215 0.39 +0.2 2,614,471 - RR42_RS32215 0.39 +1.2 2,614,471 - RR42_RS32215 0.39 -1.7 2,614,545 + RR42_RS32215 0.48 -0.4 2,614,563 - RR42_RS32215 0.50 -0.6 2,614,698 + RR42_RS32215 0.65 -0.8 2,614,699 - RR42_RS32215 0.66 +0.6 2,614,699 - RR42_RS32215 0.66 +0.6 2,614,734 + RR42_RS32215 0.70 -1.6 2,614,734 + RR42_RS32215 0.70 -1.0 2,614,735 - RR42_RS32215 0.70 +0.8 2,615,028 + -0.2 2,615,028 + -0.0 2,615,044 + +0.0 2,615,044 + +0.3 2,615,302 + RR42_RS32220 0.20 -0.9 2,615,302 + RR42_RS32220 0.20 +0.2 2,615,302 + RR42_RS32220 0.20 +0.1 2,615,303 - RR42_RS32220 0.20 -0.9 2,615,303 - RR42_RS32220 0.20 +0.1 2,615,475 + RR42_RS32220 0.35 +0.3 2,615,779 + RR42_RS32220 0.62 -0.3 2,615,780 - RR42_RS32220 0.62 -0.9 2,615,901 - RR42_RS32220 0.73 -0.3 2,615,916 - RR42_RS32220 0.74 -0.7 2,615,916 - RR42_RS32220 0.74 -2.5 2,615,964 - RR42_RS32220 0.78 -0.4
Or see this region's nucleotide sequence