Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31425 and RR42_RS31430 are separated by 129 nucleotides RR42_RS31430 and RR42_RS31435 are separated by 28 nucleotides RR42_RS31435 and RR42_RS31440 are separated by 174 nucleotides RR42_RS31440 and RR42_RS31445 are separated by 55 nucleotides
RR42_RS31425: RR42_RS31425 - chloroperoxidase, at 2,432,437 to 2,433,123
_RS31425
RR42_RS31430: RR42_RS31430 - amidohydrolase, at 2,433,253 to 2,435,124
_RS31430
RR42_RS31435: RR42_RS31435 - hypothetical protein, at 2,435,153 to 2,435,587
_RS31435
RR42_RS31440: RR42_RS31440 - ABC transporter substrate-binding protein, at 2,435,762 to 2,436,055
_RS31440
RR42_RS31445: RR42_RS31445 - porin, at 2,436,111 to 2,437,208
_RS31445
Position (kb)
2433
2434
2435
2436 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2432.266 kb on + strand at 2432.267 kb on - strand at 2432.270 kb on - strand at 2432.270 kb on - strand at 2432.306 kb on - strand at 2432.354 kb on + strand at 2432.409 kb on + strand at 2432.592 kb on + strand, within RR42_RS31425 at 2433.043 kb on + strand, within RR42_RS31425 at 2433.049 kb on + strand, within RR42_RS31425 at 2433.121 kb on + strand at 2433.130 kb on - strand at 2433.144 kb on + strand at 2433.176 kb on - strand at 2433.245 kb on + strand at 2433.246 kb on - strand at 2433.373 kb on - strand at 2433.373 kb on - strand at 2433.494 kb on - strand, within RR42_RS31430 at 2433.701 kb on - strand, within RR42_RS31430 at 2433.747 kb on + strand, within RR42_RS31430 at 2433.848 kb on - strand, within RR42_RS31430 at 2433.989 kb on - strand, within RR42_RS31430 at 2434.043 kb on - strand, within RR42_RS31430 at 2434.165 kb on + strand, within RR42_RS31430 at 2434.294 kb on + strand, within RR42_RS31430 at 2434.294 kb on + strand, within RR42_RS31430 at 2434.294 kb on + strand, within RR42_RS31430 at 2434.295 kb on - strand, within RR42_RS31430 at 2434.463 kb on - strand, within RR42_RS31430 at 2434.475 kb on - strand, within RR42_RS31430 at 2434.528 kb on + strand, within RR42_RS31430 at 2434.588 kb on + strand, within RR42_RS31430 at 2434.588 kb on + strand, within RR42_RS31430 at 2434.588 kb on + strand, within RR42_RS31430 at 2434.610 kb on - strand, within RR42_RS31430 at 2434.610 kb on - strand, within RR42_RS31430 at 2434.610 kb on - strand, within RR42_RS31430 at 2434.793 kb on - strand, within RR42_RS31430 at 2434.795 kb on + strand, within RR42_RS31430 at 2434.864 kb on + strand, within RR42_RS31430 at 2435.194 kb on + strand at 2435.253 kb on - strand, within RR42_RS31435 at 2435.664 kb on - strand at 2435.761 kb on + strand at 2435.761 kb on + strand at 2435.761 kb on + strand at 2435.762 kb on - strand at 2435.762 kb on - strand at 2435.771 kb on + strand at 2435.771 kb on + strand at 2435.772 kb on - strand at 2435.813 kb on + strand, within RR42_RS31440 at 2435.813 kb on + strand, within RR42_RS31440 at 2435.813 kb on + strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2435.814 kb on - strand, within RR42_RS31440 at 2436.035 kb on - strand at 2436.092 kb on + strand at 2436.093 kb on - strand at 2436.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,432,266 + +0.1 2,432,267 - -0.5 2,432,270 - +2.4 2,432,270 - +1.3 2,432,306 - +0.3 2,432,354 + +0.5 2,432,409 + -2.8 2,432,592 + RR42_RS31425 0.23 -0.7 2,433,043 + RR42_RS31425 0.88 -1.3 2,433,049 + RR42_RS31425 0.89 +0.1 2,433,121 + +0.0 2,433,130 - -1.0 2,433,144 + -0.1 2,433,176 - +2.0 2,433,245 + +0.3 2,433,246 - -0.9 2,433,373 - +0.7 2,433,373 - -1.8 2,433,494 - RR42_RS31430 0.13 +1.5 2,433,701 - RR42_RS31430 0.24 -1.7 2,433,747 + RR42_RS31430 0.26 -0.1 2,433,848 - RR42_RS31430 0.32 +0.0 2,433,989 - RR42_RS31430 0.39 -0.2 2,434,043 - RR42_RS31430 0.42 +0.5 2,434,165 + RR42_RS31430 0.49 -0.6 2,434,294 + RR42_RS31430 0.56 +0.3 2,434,294 + RR42_RS31430 0.56 +0.6 2,434,294 + RR42_RS31430 0.56 +1.3 2,434,295 - RR42_RS31430 0.56 +0.2 2,434,463 - RR42_RS31430 0.65 -1.6 2,434,475 - RR42_RS31430 0.65 -2.5 2,434,528 + RR42_RS31430 0.68 -0.8 2,434,588 + RR42_RS31430 0.71 -1.3 2,434,588 + RR42_RS31430 0.71 -0.8 2,434,588 + RR42_RS31430 0.71 +0.4 2,434,610 - RR42_RS31430 0.72 +0.1 2,434,610 - RR42_RS31430 0.72 +0.2 2,434,610 - RR42_RS31430 0.72 -0.3 2,434,793 - RR42_RS31430 0.82 -0.5 2,434,795 + RR42_RS31430 0.82 +0.4 2,434,864 + RR42_RS31430 0.86 -0.8 2,435,194 + -1.3 2,435,253 - RR42_RS31435 0.23 -0.7 2,435,664 - -0.6 2,435,761 + -0.3 2,435,761 + +0.4 2,435,761 + +0.4 2,435,762 - -0.2 2,435,762 - +0.1 2,435,771 + +0.3 2,435,771 + -1.2 2,435,772 - +0.1 2,435,813 + RR42_RS31440 0.17 -1.3 2,435,813 + RR42_RS31440 0.17 -0.3 2,435,813 + RR42_RS31440 0.17 +0.5 2,435,814 - RR42_RS31440 0.18 -1.0 2,435,814 - RR42_RS31440 0.18 -1.0 2,435,814 - RR42_RS31440 0.18 -1.0 2,435,814 - RR42_RS31440 0.18 +0.4 2,435,814 - RR42_RS31440 0.18 -0.9 2,435,814 - RR42_RS31440 0.18 +2.7 2,435,814 - RR42_RS31440 0.18 -1.5 2,435,814 - RR42_RS31440 0.18 -0.0 2,436,035 - +0.6 2,436,092 + +0.2 2,436,093 - -0.1 2,436,093 - -0.3
Or see this region's nucleotide sequence