Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30170 and RR42_RS30175 are separated by 154 nucleotides RR42_RS30175 and RR42_RS30180 are separated by 81 nucleotides
RR42_RS30170: RR42_RS30170 - aldolase, at 2,142,844 to 2,143,608
_RS30170
RR42_RS30175: RR42_RS30175 - beta-glucosidase, at 2,143,763 to 2,145,889
_RS30175
RR42_RS30180: RR42_RS30180 - hypothetical protein, at 2,145,971 to 2,147,392
_RS30180
Position (kb)
2143
2144
2145
2146 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2142.845 kb on + strand at 2142.846 kb on - strand at 2142.846 kb on - strand at 2143.034 kb on + strand, within RR42_RS30170 at 2143.034 kb on + strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.266 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.600 kb on - strand at 2143.630 kb on + strand at 2143.731 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.794 kb on - strand at 2143.915 kb on + strand at 2144.045 kb on - strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.119 kb on - strand, within RR42_RS30175 at 2144.202 kb on + strand, within RR42_RS30175 at 2144.323 kb on + strand, within RR42_RS30175 at 2144.539 kb on - strand, within RR42_RS30175 at 2144.863 kb on + strand, within RR42_RS30175 at 2144.863 kb on + strand, within RR42_RS30175 at 2144.864 kb on - strand, within RR42_RS30175 at 2144.956 kb on - strand, within RR42_RS30175 at 2145.112 kb on - strand, within RR42_RS30175 at 2145.112 kb on - strand, within RR42_RS30175 at 2145.201 kb on + strand, within RR42_RS30175 at 2145.201 kb on + strand, within RR42_RS30175 at 2145.202 kb on - strand, within RR42_RS30175 at 2145.222 kb on + strand, within RR42_RS30175 at 2145.223 kb on - strand, within RR42_RS30175 at 2145.223 kb on - strand, within RR42_RS30175 at 2145.510 kb on - strand, within RR42_RS30175 at 2145.707 kb on + strand at 2145.707 kb on + strand at 2145.708 kb on - strand at 2145.708 kb on - strand at 2146.407 kb on + strand, within RR42_RS30180 at 2146.557 kb on + strand, within RR42_RS30180 at 2146.570 kb on - strand, within RR42_RS30180 at 2146.761 kb on + strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180 at 2146.762 kb on - strand, within RR42_RS30180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,142,845 + +0.8 2,142,846 - -0.9 2,142,846 - +2.3 2,143,034 + RR42_RS30170 0.25 +0.4 2,143,034 + RR42_RS30170 0.25 -0.6 2,143,035 - RR42_RS30170 0.25 -0.7 2,143,035 - RR42_RS30170 0.25 +0.1 2,143,035 - RR42_RS30170 0.25 +0.2 2,143,266 + RR42_RS30170 0.55 +0.3 2,143,272 + RR42_RS30170 0.56 +2.1 2,143,272 + RR42_RS30170 0.56 -1.0 2,143,600 - +0.9 2,143,630 + +0.4 2,143,731 + +0.4 2,143,793 + -0.7 2,143,793 + -0.0 2,143,793 + +0.5 2,143,794 - +0.2 2,143,915 + +1.0 2,144,045 - RR42_RS30175 0.13 -1.0 2,144,118 + RR42_RS30175 0.17 -0.7 2,144,118 + RR42_RS30175 0.17 -0.2 2,144,118 + RR42_RS30175 0.17 -0.6 2,144,118 + RR42_RS30175 0.17 -0.4 2,144,119 - RR42_RS30175 0.17 +0.3 2,144,202 + RR42_RS30175 0.21 -0.6 2,144,323 + RR42_RS30175 0.26 +1.3 2,144,539 - RR42_RS30175 0.36 -0.1 2,144,863 + RR42_RS30175 0.52 +0.7 2,144,863 + RR42_RS30175 0.52 -0.6 2,144,864 - RR42_RS30175 0.52 +0.4 2,144,956 - RR42_RS30175 0.56 -0.7 2,145,112 - RR42_RS30175 0.63 -0.2 2,145,112 - RR42_RS30175 0.63 +0.2 2,145,201 + RR42_RS30175 0.68 +0.4 2,145,201 + RR42_RS30175 0.68 +0.2 2,145,202 - RR42_RS30175 0.68 -0.4 2,145,222 + RR42_RS30175 0.69 -0.5 2,145,223 - RR42_RS30175 0.69 +0.6 2,145,223 - RR42_RS30175 0.69 -2.2 2,145,510 - RR42_RS30175 0.82 -2.2 2,145,707 + -0.7 2,145,707 + +1.5 2,145,708 - -0.5 2,145,708 - -0.1 2,146,407 + RR42_RS30180 0.31 -0.1 2,146,557 + RR42_RS30180 0.41 +0.4 2,146,570 - RR42_RS30180 0.42 -0.4 2,146,761 + RR42_RS30180 0.56 +0.2 2,146,762 - RR42_RS30180 0.56 +0.2 2,146,762 - RR42_RS30180 0.56 -0.3 2,146,762 - RR42_RS30180 0.56 -0.7 2,146,762 - RR42_RS30180 0.56 -0.6 2,146,762 - RR42_RS30180 0.56 +0.0 2,146,762 - RR42_RS30180 0.56 +1.7
Or see this region's nucleotide sequence