Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29745 and RR42_RS29750 are separated by 267 nucleotides RR42_RS29750 and RR42_RS29755 are separated by 93 nucleotides RR42_RS29755 and RR42_RS29760 are separated by 12 nucleotides
RR42_RS29745: RR42_RS29745 - peptidase M15, at 2,042,715 to 2,043,437
_RS29745
RR42_RS29750: RR42_RS29750 - MarR family transcriptional regulator, at 2,043,705 to 2,044,085
_RS29750
RR42_RS29755: RR42_RS29755 - membrane protein, at 2,044,179 to 2,044,583
_RS29755
RR42_RS29760: RR42_RS29760 - 3-oxoacyl-ACP reductase, at 2,044,596 to 2,045,327
_RS29760
Position (kb)
2043
2044
2045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2042.712 kb on + strand at 2042.773 kb on + strand at 2042.788 kb on + strand, within RR42_RS29745 at 2042.792 kb on + strand, within RR42_RS29745 at 2042.793 kb on - strand, within RR42_RS29745 at 2042.808 kb on + strand, within RR42_RS29745 at 2042.808 kb on + strand, within RR42_RS29745 at 2042.809 kb on - strand, within RR42_RS29745 at 2042.809 kb on - strand, within RR42_RS29745 at 2042.809 kb on - strand, within RR42_RS29745 at 2042.809 kb on - strand, within RR42_RS29745 at 2042.950 kb on + strand, within RR42_RS29745 at 2042.950 kb on + strand, within RR42_RS29745 at 2042.950 kb on + strand, within RR42_RS29745 at 2042.950 kb on + strand, within RR42_RS29745 at 2042.950 kb on + strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2042.951 kb on - strand, within RR42_RS29745 at 2043.006 kb on - strand, within RR42_RS29745 at 2043.006 kb on - strand, within RR42_RS29745 at 2043.051 kb on + strand, within RR42_RS29745 at 2043.052 kb on - strand, within RR42_RS29745 at 2043.064 kb on + strand, within RR42_RS29745 at 2043.065 kb on - strand, within RR42_RS29745 at 2043.104 kb on + strand, within RR42_RS29745 at 2043.105 kb on - strand, within RR42_RS29745 at 2043.121 kb on - strand, within RR42_RS29745 at 2043.180 kb on + strand, within RR42_RS29745 at 2043.180 kb on + strand, within RR42_RS29745 at 2043.213 kb on + strand, within RR42_RS29745 at 2043.213 kb on + strand, within RR42_RS29745 at 2043.214 kb on - strand, within RR42_RS29745 at 2043.263 kb on + strand, within RR42_RS29745 at 2043.288 kb on - strand, within RR42_RS29745 at 2043.288 kb on - strand, within RR42_RS29745 at 2043.303 kb on + strand, within RR42_RS29745 at 2043.304 kb on - strand, within RR42_RS29745 at 2043.304 kb on - strand, within RR42_RS29745 at 2043.304 kb on - strand, within RR42_RS29745 at 2043.500 kb on - strand at 2043.501 kb on + strand at 2043.530 kb on + strand at 2043.589 kb on + strand at 2043.604 kb on + strand at 2043.605 kb on - strand at 2043.621 kb on - strand at 2043.706 kb on + strand at 2043.706 kb on + strand at 2043.706 kb on + strand at 2043.707 kb on - strand at 2043.763 kb on + strand, within RR42_RS29750 at 2043.763 kb on + strand, within RR42_RS29750 at 2043.763 kb on + strand, within RR42_RS29750 at 2043.764 kb on - strand, within RR42_RS29750 at 2043.876 kb on + strand, within RR42_RS29750 at 2043.876 kb on + strand, within RR42_RS29750 at 2043.876 kb on + strand, within RR42_RS29750 at 2043.936 kb on + strand, within RR42_RS29750 at 2043.964 kb on + strand, within RR42_RS29750 at 2043.965 kb on - strand, within RR42_RS29750 at 2043.965 kb on - strand, within RR42_RS29750 at 2044.070 kb on + strand at 2044.070 kb on + strand at 2044.085 kb on + strand at 2044.086 kb on - strand at 2044.088 kb on + strand at 2044.088 kb on + strand at 2044.088 kb on + strand at 2044.088 kb on + strand at 2044.089 kb on - strand at 2044.089 kb on - strand at 2044.089 kb on - strand at 2044.108 kb on + strand at 2044.109 kb on - strand at 2044.109 kb on - strand at 2044.123 kb on - strand at 2044.127 kb on + strand at 2044.127 kb on + strand at 2044.168 kb on + strand at 2044.169 kb on - strand at 2044.169 kb on - strand at 2044.191 kb on + strand at 2044.191 kb on + strand at 2044.192 kb on - strand at 2044.192 kb on - strand at 2044.192 kb on - strand at 2044.197 kb on + strand at 2044.250 kb on + strand, within RR42_RS29755 at 2044.251 kb on + strand, within RR42_RS29755 at 2044.251 kb on + strand, within RR42_RS29755 at 2044.251 kb on + strand, within RR42_RS29755 at 2044.251 kb on + strand, within RR42_RS29755 at 2044.252 kb on - strand, within RR42_RS29755 at 2044.252 kb on - strand, within RR42_RS29755 at 2044.256 kb on - strand, within RR42_RS29755 at 2044.286 kb on + strand, within RR42_RS29755 at 2044.287 kb on - strand, within RR42_RS29755 at 2044.299 kb on + strand, within RR42_RS29755 at 2044.315 kb on + strand, within RR42_RS29755 at 2044.316 kb on - strand, within RR42_RS29755 at 2044.381 kb on + strand, within RR42_RS29755 at 2044.410 kb on + strand, within RR42_RS29755 at 2044.545 kb on + strand at 2044.545 kb on + strand at 2044.546 kb on - strand at 2044.546 kb on - strand at 2044.546 kb on - strand at 2044.581 kb on + strand at 2044.582 kb on - strand at 2044.809 kb on - strand, within RR42_RS29760 at 2044.820 kb on + strand, within RR42_RS29760 at 2044.821 kb on - strand, within RR42_RS29760 at 2044.824 kb on + strand, within RR42_RS29760 at 2044.824 kb on + strand, within RR42_RS29760 at 2044.825 kb on - strand, within RR42_RS29760 at 2044.825 kb on - strand, within RR42_RS29760 at 2044.942 kb on - strand, within RR42_RS29760 at 2044.942 kb on - strand, within RR42_RS29760 at 2044.966 kb on - strand, within RR42_RS29760 at 2045.043 kb on + strand, within RR42_RS29760 at 2045.052 kb on + strand, within RR42_RS29760 at 2045.053 kb on - strand, within RR42_RS29760 at 2045.053 kb on - strand, within RR42_RS29760 at 2045.053 kb on - strand, within RR42_RS29760 at 2045.053 kb on - strand, within RR42_RS29760 at 2045.053 kb on - strand, within RR42_RS29760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,042,712 + -1.1 2,042,773 + -0.5 2,042,788 + RR42_RS29745 0.10 -0.7 2,042,792 + RR42_RS29745 0.11 -0.6 2,042,793 - RR42_RS29745 0.11 -0.8 2,042,808 + RR42_RS29745 0.13 -3.5 2,042,808 + RR42_RS29745 0.13 -2.7 2,042,809 - RR42_RS29745 0.13 -0.7 2,042,809 - RR42_RS29745 0.13 -1.0 2,042,809 - RR42_RS29745 0.13 -0.6 2,042,809 - RR42_RS29745 0.13 -0.6 2,042,950 + RR42_RS29745 0.33 +0.7 2,042,950 + RR42_RS29745 0.33 -1.6 2,042,950 + RR42_RS29745 0.33 -0.6 2,042,950 + RR42_RS29745 0.33 -0.3 2,042,950 + RR42_RS29745 0.33 -1.8 2,042,951 - RR42_RS29745 0.33 +0.7 2,042,951 - RR42_RS29745 0.33 -1.2 2,042,951 - RR42_RS29745 0.33 -0.2 2,042,951 - RR42_RS29745 0.33 +0.6 2,042,951 - RR42_RS29745 0.33 -0.4 2,042,951 - RR42_RS29745 0.33 -0.1 2,043,006 - RR42_RS29745 0.40 -0.3 2,043,006 - RR42_RS29745 0.40 -1.1 2,043,051 + RR42_RS29745 0.46 -1.0 2,043,052 - RR42_RS29745 0.47 +0.2 2,043,064 + RR42_RS29745 0.48 +0.5 2,043,065 - RR42_RS29745 0.48 -2.2 2,043,104 + RR42_RS29745 0.54 -1.2 2,043,105 - RR42_RS29745 0.54 +0.5 2,043,121 - RR42_RS29745 0.56 -0.6 2,043,180 + RR42_RS29745 0.64 -1.6 2,043,180 + RR42_RS29745 0.64 -1.2 2,043,213 + RR42_RS29745 0.69 -0.9 2,043,213 + RR42_RS29745 0.69 -1.5 2,043,214 - RR42_RS29745 0.69 -1.8 2,043,263 + RR42_RS29745 0.76 -2.6 2,043,288 - RR42_RS29745 0.79 -0.9 2,043,288 - RR42_RS29745 0.79 +0.2 2,043,303 + RR42_RS29745 0.81 -0.1 2,043,304 - RR42_RS29745 0.81 -0.2 2,043,304 - RR42_RS29745 0.81 +0.6 2,043,304 - RR42_RS29745 0.81 -0.3 2,043,500 - -0.4 2,043,501 + -0.9 2,043,530 + -0.7 2,043,589 + -1.3 2,043,604 + -0.6 2,043,605 - +0.1 2,043,621 - -2.7 2,043,706 + -2.9 2,043,706 + +1.3 2,043,706 + -0.3 2,043,707 - -2.1 2,043,763 + RR42_RS29750 0.15 -1.3 2,043,763 + RR42_RS29750 0.15 +0.0 2,043,763 + RR42_RS29750 0.15 -0.9 2,043,764 - RR42_RS29750 0.15 -0.8 2,043,876 + RR42_RS29750 0.45 -0.2 2,043,876 + RR42_RS29750 0.45 -1.4 2,043,876 + RR42_RS29750 0.45 -0.9 2,043,936 + RR42_RS29750 0.61 -1.5 2,043,964 + RR42_RS29750 0.68 +0.1 2,043,965 - RR42_RS29750 0.68 +0.2 2,043,965 - RR42_RS29750 0.68 +2.3 2,044,070 + -0.7 2,044,070 + -0.2 2,044,085 + -1.5 2,044,086 - -1.5 2,044,088 + +0.4 2,044,088 + +0.8 2,044,088 + -0.7 2,044,088 + -0.0 2,044,089 - -1.3 2,044,089 - -0.3 2,044,089 - -1.8 2,044,108 + +1.2 2,044,109 - -0.5 2,044,109 - -0.8 2,044,123 - -0.6 2,044,127 + +1.7 2,044,127 + +0.0 2,044,168 + -1.8 2,044,169 - -2.0 2,044,169 - -2.2 2,044,191 + -1.2 2,044,191 + -2.6 2,044,192 - +0.3 2,044,192 - -0.0 2,044,192 - +0.1 2,044,197 + -0.1 2,044,250 + RR42_RS29755 0.18 -1.3 2,044,251 + RR42_RS29755 0.18 -0.9 2,044,251 + RR42_RS29755 0.18 +0.3 2,044,251 + RR42_RS29755 0.18 -2.2 2,044,251 + RR42_RS29755 0.18 -1.2 2,044,252 - RR42_RS29755 0.18 +0.2 2,044,252 - RR42_RS29755 0.18 -0.3 2,044,256 - RR42_RS29755 0.19 -1.4 2,044,286 + RR42_RS29755 0.26 -0.6 2,044,287 - RR42_RS29755 0.27 -0.8 2,044,299 + RR42_RS29755 0.30 -0.5 2,044,315 + RR42_RS29755 0.34 +0.5 2,044,316 - RR42_RS29755 0.34 -0.9 2,044,381 + RR42_RS29755 0.50 +0.5 2,044,410 + RR42_RS29755 0.57 -2.7 2,044,545 + +1.7 2,044,545 + +0.6 2,044,546 - -0.4 2,044,546 - -0.1 2,044,546 - +0.4 2,044,581 + +0.2 2,044,582 - -0.4 2,044,809 - RR42_RS29760 0.29 -0.9 2,044,820 + RR42_RS29760 0.31 -1.4 2,044,821 - RR42_RS29760 0.31 -0.8 2,044,824 + RR42_RS29760 0.31 -0.5 2,044,824 + RR42_RS29760 0.31 +0.8 2,044,825 - RR42_RS29760 0.31 +0.9 2,044,825 - RR42_RS29760 0.31 -0.3 2,044,942 - RR42_RS29760 0.47 -0.7 2,044,942 - RR42_RS29760 0.47 +0.2 2,044,966 - RR42_RS29760 0.51 +0.2 2,045,043 + RR42_RS29760 0.61 +0.8 2,045,052 + RR42_RS29760 0.62 -0.8 2,045,053 - RR42_RS29760 0.62 +0.2 2,045,053 - RR42_RS29760 0.62 -1.3 2,045,053 - RR42_RS29760 0.62 -1.9 2,045,053 - RR42_RS29760 0.62 +0.1 2,045,053 - RR42_RS29760 0.62 -2.7
Or see this region's nucleotide sequence