Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29650 and RR42_RS29655 are separated by 3 nucleotides RR42_RS29655 and RR42_RS29660 are separated by 59 nucleotides RR42_RS29660 and RR42_RS29665 are separated by 170 nucleotides
RR42_RS29650: RR42_RS29650 - histidine ABC transporter permease, at 2,020,144 to 2,020,833
_RS29650
RR42_RS29655: RR42_RS29655 - amino acid ABC transporter permease, at 2,020,837 to 2,021,550
_RS29655
RR42_RS29660: RR42_RS29660 - amino acid transporter, at 2,021,610 to 2,022,404
_RS29660
RR42_RS29665: RR42_RS29665 - AsnC family transcriptional regulator, at 2,022,575 to 2,022,994
_RS29665
Position (kb)
2021
2022
2023 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2020.748 kb on - strand, within RR42_RS29650 at 2020.748 kb on - strand, within RR42_RS29650 at 2020.756 kb on - strand, within RR42_RS29650 at 2020.801 kb on + strand at 2020.801 kb on + strand at 2020.802 kb on - strand at 2020.802 kb on - strand at 2020.831 kb on + strand at 2020.832 kb on - strand at 2020.845 kb on + strand at 2020.877 kb on - strand at 2020.951 kb on - strand, within RR42_RS29655 at 2020.980 kb on + strand, within RR42_RS29655 at 2020.980 kb on + strand, within RR42_RS29655 at 2020.981 kb on - strand, within RR42_RS29655 at 2021.021 kb on - strand, within RR42_RS29655 at 2021.021 kb on - strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.031 kb on - strand, within RR42_RS29655 at 2021.031 kb on - strand, within RR42_RS29655 at 2021.072 kb on - strand, within RR42_RS29655 at 2021.072 kb on - strand, within RR42_RS29655 at 2021.140 kb on + strand, within RR42_RS29655 at 2021.143 kb on + strand, within RR42_RS29655 at 2021.144 kb on - strand, within RR42_RS29655 at 2021.144 kb on - strand, within RR42_RS29655 at 2021.182 kb on + strand, within RR42_RS29655 at 2021.276 kb on - strand, within RR42_RS29655 at 2021.323 kb on + strand, within RR42_RS29655 at 2021.324 kb on - strand, within RR42_RS29655 at 2021.355 kb on + strand, within RR42_RS29655 at 2021.355 kb on + strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.464 kb on + strand, within RR42_RS29655 at 2021.465 kb on - strand, within RR42_RS29655 at 2021.528 kb on - strand at 2021.528 kb on - strand at 2021.528 kb on - strand at 2021.548 kb on + strand at 2021.548 kb on + strand at 2021.549 kb on - strand at 2021.549 kb on - strand at 2021.615 kb on - strand at 2021.615 kb on - strand at 2021.689 kb on + strand at 2021.689 kb on + strand at 2021.689 kb on + strand at 2021.726 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2022.033 kb on + strand, within RR42_RS29660 at 2022.033 kb on + strand, within RR42_RS29660 at 2022.034 kb on - strand, within RR42_RS29660 at 2022.075 kb on + strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.205 kb on + strand, within RR42_RS29660 at 2022.206 kb on - strand, within RR42_RS29660 at 2022.206 kb on - strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.254 kb on + strand, within RR42_RS29660 at 2022.304 kb on + strand, within RR42_RS29660 at 2022.305 kb on - strand, within RR42_RS29660 at 2022.318 kb on - strand, within RR42_RS29660 at 2022.402 kb on + strand at 2022.403 kb on - strand at 2022.414 kb on + strand at 2022.414 kb on + strand at 2022.414 kb on + strand at 2022.468 kb on - strand at 2022.494 kb on + strand at 2022.529 kb on + strand at 2022.530 kb on - strand at 2022.607 kb on - strand at 2022.687 kb on + strand, within RR42_RS29665 at 2022.688 kb on - strand, within RR42_RS29665 at 2022.722 kb on + strand, within RR42_RS29665 at 2022.723 kb on - strand, within RR42_RS29665 at 2022.780 kb on + strand, within RR42_RS29665 at 2022.781 kb on - strand, within RR42_RS29665 at 2022.786 kb on + strand, within RR42_RS29665 at 2022.787 kb on - strand, within RR42_RS29665 at 2022.888 kb on + strand, within RR42_RS29665 at 2022.889 kb on - strand, within RR42_RS29665 at 2022.921 kb on + strand, within RR42_RS29665 at 2022.922 kb on - strand, within RR42_RS29665 at 2022.924 kb on + strand, within RR42_RS29665 at 2022.925 kb on - strand, within RR42_RS29665 at 2022.934 kb on - strand, within RR42_RS29665 at 2022.943 kb on + strand, within RR42_RS29665 at 2022.958 kb on + strand at 2022.993 kb on - strand at 2022.996 kb on + strand at 2022.996 kb on + strand at 2022.997 kb on - strand at 2022.997 kb on - strand at 2023.000 kb on - strand at 2023.022 kb on + strand at 2023.022 kb on + strand at 2023.022 kb on + strand at 2023.022 kb on + strand at 2023.023 kb on - strand at 2023.023 kb on - strand at 2023.026 kb on + strand at 2023.039 kb on + strand at 2023.040 kb on - strand at 2023.068 kb on + strand at 2023.068 kb on + strand at 2023.069 kb on - strand at 2023.069 kb on - strand at 2023.069 kb on - strand at 2023.069 kb on - strand at 2023.070 kb on + strand at 2023.071 kb on - strand at 2023.110 kb on + strand at 2023.110 kb on + strand at 2023.111 kb on - strand at 2023.129 kb on - strand at 2023.166 kb on - strand at 2023.174 kb on - strand at 2023.192 kb on + strand at 2023.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,020,748 - RR42_RS29650 0.88 -1.1 2,020,748 - RR42_RS29650 0.88 -1.9 2,020,756 - RR42_RS29650 0.89 -1.1 2,020,801 + -0.1 2,020,801 + -1.8 2,020,802 - -1.8 2,020,802 - -2.3 2,020,831 + +0.4 2,020,832 - +0.9 2,020,845 + -1.7 2,020,877 - -1.6 2,020,951 - RR42_RS29655 0.16 -1.1 2,020,980 + RR42_RS29655 0.20 -1.9 2,020,980 + RR42_RS29655 0.20 -2.5 2,020,981 - RR42_RS29655 0.20 -1.2 2,021,021 - RR42_RS29655 0.26 -2.2 2,021,021 - RR42_RS29655 0.26 -1.1 2,021,026 + RR42_RS29655 0.26 -0.0 2,021,026 + RR42_RS29655 0.26 -1.5 2,021,026 + RR42_RS29655 0.26 -0.2 2,021,026 + RR42_RS29655 0.26 -1.8 2,021,027 - RR42_RS29655 0.27 +1.8 2,021,027 - RR42_RS29655 0.27 -1.0 2,021,027 - RR42_RS29655 0.27 -0.8 2,021,031 - RR42_RS29655 0.27 +0.4 2,021,031 - RR42_RS29655 0.27 -0.9 2,021,072 - RR42_RS29655 0.33 -1.6 2,021,072 - RR42_RS29655 0.33 -1.9 2,021,140 + RR42_RS29655 0.42 -1.2 2,021,143 + RR42_RS29655 0.43 +0.3 2,021,144 - RR42_RS29655 0.43 -0.8 2,021,144 - RR42_RS29655 0.43 -0.0 2,021,182 + RR42_RS29655 0.48 -1.5 2,021,276 - RR42_RS29655 0.61 -0.7 2,021,323 + RR42_RS29655 0.68 -1.5 2,021,324 - RR42_RS29655 0.68 -0.8 2,021,355 + RR42_RS29655 0.73 -0.9 2,021,355 + RR42_RS29655 0.73 -1.0 2,021,356 - RR42_RS29655 0.73 -0.8 2,021,356 - RR42_RS29655 0.73 -0.6 2,021,356 - RR42_RS29655 0.73 -0.8 2,021,356 - RR42_RS29655 0.73 -0.5 2,021,356 - RR42_RS29655 0.73 -1.2 2,021,425 + RR42_RS29655 0.82 -1.0 2,021,425 + RR42_RS29655 0.82 -2.7 2,021,425 + RR42_RS29655 0.82 -0.7 2,021,425 + RR42_RS29655 0.82 -0.2 2,021,426 - RR42_RS29655 0.82 +0.7 2,021,426 - RR42_RS29655 0.82 -0.6 2,021,426 - RR42_RS29655 0.82 -1.2 2,021,426 - RR42_RS29655 0.82 -2.4 2,021,426 - RR42_RS29655 0.82 -0.3 2,021,426 - RR42_RS29655 0.82 -1.0 2,021,464 + RR42_RS29655 0.88 -1.1 2,021,465 - RR42_RS29655 0.88 -1.2 2,021,528 - -0.8 2,021,528 - +0.4 2,021,528 - +0.3 2,021,548 + -0.1 2,021,548 + -1.0 2,021,549 - -1.6 2,021,549 - -1.4 2,021,615 - -1.5 2,021,615 - -2.5 2,021,689 + -0.9 2,021,689 + -1.7 2,021,689 + +0.1 2,021,726 + RR42_RS29660 0.15 -0.9 2,021,732 + RR42_RS29660 0.15 -2.3 2,021,732 + RR42_RS29660 0.15 -0.8 2,021,732 + RR42_RS29660 0.15 -2.2 2,022,033 + RR42_RS29660 0.53 -1.4 2,022,033 + RR42_RS29660 0.53 -1.3 2,022,034 - RR42_RS29660 0.53 -0.5 2,022,075 + RR42_RS29660 0.58 -2.1 2,022,076 - RR42_RS29660 0.59 -1.6 2,022,076 - RR42_RS29660 0.59 +0.7 2,022,076 - RR42_RS29660 0.59 +2.0 2,022,167 + RR42_RS29660 0.70 -1.2 2,022,167 + RR42_RS29660 0.70 +0.9 2,022,167 + RR42_RS29660 0.70 -0.5 2,022,168 - RR42_RS29660 0.70 -0.2 2,022,168 - RR42_RS29660 0.70 -0.6 2,022,168 - RR42_RS29660 0.70 -0.3 2,022,205 + RR42_RS29660 0.75 -0.8 2,022,206 - RR42_RS29660 0.75 -1.6 2,022,206 - RR42_RS29660 0.75 -2.2 2,022,250 + RR42_RS29660 0.81 -0.6 2,022,250 + RR42_RS29660 0.81 +1.0 2,022,250 + RR42_RS29660 0.81 +0.2 2,022,250 + RR42_RS29660 0.81 -1.2 2,022,250 + RR42_RS29660 0.81 -0.8 2,022,250 + RR42_RS29660 0.81 -0.0 2,022,251 - RR42_RS29660 0.81 -0.6 2,022,251 - RR42_RS29660 0.81 -0.7 2,022,251 - RR42_RS29660 0.81 +0.0 2,022,251 - RR42_RS29660 0.81 -0.2 2,022,254 + RR42_RS29660 0.81 -1.2 2,022,304 + RR42_RS29660 0.87 -1.2 2,022,305 - RR42_RS29660 0.87 +1.5 2,022,318 - RR42_RS29660 0.89 -1.6 2,022,402 + -1.5 2,022,403 - -0.7 2,022,414 + +0.7 2,022,414 + +0.1 2,022,414 + -1.3 2,022,468 - -1.2 2,022,494 + -0.1 2,022,529 + -1.0 2,022,530 - -1.1 2,022,607 - -1.6 2,022,687 + RR42_RS29665 0.27 -0.7 2,022,688 - RR42_RS29665 0.27 +0.1 2,022,722 + RR42_RS29665 0.35 -2.1 2,022,723 - RR42_RS29665 0.35 -1.9 2,022,780 + RR42_RS29665 0.49 -2.6 2,022,781 - RR42_RS29665 0.49 -2.3 2,022,786 + RR42_RS29665 0.50 -1.4 2,022,787 - RR42_RS29665 0.50 -1.3 2,022,888 + RR42_RS29665 0.75 -1.8 2,022,889 - RR42_RS29665 0.75 +0.4 2,022,921 + RR42_RS29665 0.82 -0.6 2,022,922 - RR42_RS29665 0.83 +0.5 2,022,924 + RR42_RS29665 0.83 +0.6 2,022,925 - RR42_RS29665 0.83 -0.3 2,022,934 - RR42_RS29665 0.85 +0.5 2,022,943 + RR42_RS29665 0.88 -1.1 2,022,958 + +0.2 2,022,993 - -0.0 2,022,996 + -0.1 2,022,996 + -0.5 2,022,997 - -0.8 2,022,997 - -1.3 2,023,000 - -3.1 2,023,022 + -0.5 2,023,022 + -0.2 2,023,022 + +0.1 2,023,022 + -2.2 2,023,023 - -0.6 2,023,023 - -1.5 2,023,026 + -1.9 2,023,039 + -1.3 2,023,040 - -1.6 2,023,068 + -2.0 2,023,068 + +0.4 2,023,069 - -0.7 2,023,069 - -1.5 2,023,069 - -1.1 2,023,069 - -0.2 2,023,070 + -0.9 2,023,071 - -0.9 2,023,110 + -1.5 2,023,110 + -0.9 2,023,111 - -0.8 2,023,129 - -1.2 2,023,166 - -0.9 2,023,174 - -1.7 2,023,192 + -0.1 2,023,193 - -1.1
Or see this region's nucleotide sequence