Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29375

Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29370 and RR42_RS29375 are separated by 26 nucleotidesRR42_RS29375 and RR42_RS29380 are separated by 15 nucleotidesRR42_RS29380 and RR42_RS29385 are separated by 402 nucleotides RR42_RS29370: RR42_RS29370 - chromate transporter, at 1,940,451 to 1,941,677 _RS29370 RR42_RS29375: RR42_RS29375 - arsenite oxidase large subunit, at 1,941,704 to 1,944,190 _RS29375 RR42_RS29380: RR42_RS29380 - arsenite oxidase small subunit, at 1,944,206 to 1,944,724 _RS29380 RR42_RS29385: RR42_RS29385 - long-chain fatty acid--CoA ligase, at 1,945,127 to 1,946,695 _RS29385 Position (kb) 1941 1942 1943 1944 1945Strain fitness (log2 ratio) -2 -1 0 1 2at 1940.909 kb on + strand, within RR42_RS29370at 1941.032 kb on - strand, within RR42_RS29370at 1941.157 kb on + strand, within RR42_RS29370at 1941.157 kb on + strand, within RR42_RS29370at 1941.171 kb on + strand, within RR42_RS29370at 1941.171 kb on + strand, within RR42_RS29370at 1941.171 kb on + strand, within RR42_RS29370at 1941.310 kb on + strand, within RR42_RS29370at 1941.310 kb on + strand, within RR42_RS29370at 1941.412 kb on + strand, within RR42_RS29370at 1941.413 kb on - strand, within RR42_RS29370at 1941.509 kb on - strand, within RR42_RS29370at 1941.509 kb on - strand, within RR42_RS29370at 1941.526 kb on - strand, within RR42_RS29370at 1941.526 kb on - strand, within RR42_RS29370at 1941.798 kb on + strandat 1941.798 kb on + strandat 1941.798 kb on + strandat 1941.798 kb on + strandat 1941.799 kb on - strandat 1941.799 kb on - strandat 1941.910 kb on - strandat 1941.910 kb on - strandat 1941.921 kb on + strandat 1941.921 kb on + strandat 1941.921 kb on + strandat 1941.922 kb on - strandat 1941.922 kb on - strandat 1941.922 kb on - strandat 1941.942 kb on + strandat 1941.943 kb on - strandat 1942.009 kb on - strand, within RR42_RS29375at 1942.056 kb on + strand, within RR42_RS29375at 1942.057 kb on - strand, within RR42_RS29375at 1942.107 kb on + strand, within RR42_RS29375at 1942.107 kb on + strand, within RR42_RS29375at 1942.107 kb on + strand, within RR42_RS29375at 1942.107 kb on + strand, within RR42_RS29375at 1942.108 kb on - strand, within RR42_RS29375at 1942.206 kb on + strand, within RR42_RS29375at 1942.207 kb on - strand, within RR42_RS29375at 1942.207 kb on - strand, within RR42_RS29375at 1942.243 kb on - strand, within RR42_RS29375at 1942.297 kb on - strand, within RR42_RS29375at 1942.297 kb on - strand, within RR42_RS29375at 1942.297 kb on - strand, within RR42_RS29375at 1942.453 kb on - strand, within RR42_RS29375at 1942.453 kb on - strand, within RR42_RS29375at 1942.453 kb on - strand, within RR42_RS29375at 1942.453 kb on - strand, within RR42_RS29375at 1942.469 kb on - strand, within RR42_RS29375at 1942.478 kb on - strand, within RR42_RS29375at 1942.639 kb on - strand, within RR42_RS29375at 1942.639 kb on - strand, within RR42_RS29375at 1942.689 kb on + strand, within RR42_RS29375at 1942.689 kb on + strand, within RR42_RS29375at 1942.690 kb on - strand, within RR42_RS29375at 1942.833 kb on + strand, within RR42_RS29375at 1942.833 kb on + strand, within RR42_RS29375at 1942.833 kb on + strand, within RR42_RS29375at 1942.834 kb on - strand, within RR42_RS29375at 1942.834 kb on - strand, within RR42_RS29375at 1942.872 kb on + strand, within RR42_RS29375at 1942.873 kb on - strand, within RR42_RS29375at 1942.894 kb on - strand, within RR42_RS29375at 1943.007 kb on + strand, within RR42_RS29375at 1943.007 kb on + strand, within RR42_RS29375at 1943.007 kb on + strand, within RR42_RS29375at 1943.007 kb on + strand, within RR42_RS29375at 1943.007 kb on + strand, within RR42_RS29375at 1943.008 kb on - strand, within RR42_RS29375at 1943.008 kb on - strand, within RR42_RS29375at 1943.040 kb on + strand, within RR42_RS29375at 1943.053 kb on + strand, within RR42_RS29375at 1943.054 kb on - strand, within RR42_RS29375at 1943.086 kb on - strand, within RR42_RS29375at 1943.086 kb on - strand, within RR42_RS29375at 1943.194 kb on + strand, within RR42_RS29375at 1943.194 kb on + strand, within RR42_RS29375at 1943.194 kb on + strand, within RR42_RS29375at 1943.195 kb on - strand, within RR42_RS29375at 1943.195 kb on - strand, within RR42_RS29375at 1943.195 kb on - strand, within RR42_RS29375at 1943.196 kb on + strand, within RR42_RS29375at 1943.463 kb on + strand, within RR42_RS29375at 1943.463 kb on + strand, within RR42_RS29375at 1943.464 kb on - strand, within RR42_RS29375at 1943.464 kb on - strand, within RR42_RS29375at 1943.481 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.532 kb on + strand, within RR42_RS29375at 1943.533 kb on - strand, within RR42_RS29375at 1943.533 kb on - strand, within RR42_RS29375at 1943.589 kb on + strand, within RR42_RS29375at 1943.590 kb on - strand, within RR42_RS29375at 1943.590 kb on - strand, within RR42_RS29375at 1943.750 kb on + strand, within RR42_RS29375at 1943.788 kb on + strand, within RR42_RS29375at 1943.809 kb on + strand, within RR42_RS29375at 1943.810 kb on - strand, within RR42_RS29375at 1943.810 kb on - strand, within RR42_RS29375at 1943.811 kb on + strand, within RR42_RS29375at 1943.811 kb on + strand, within RR42_RS29375at 1943.811 kb on + strand, within RR42_RS29375at 1943.812 kb on - strand, within RR42_RS29375at 1943.812 kb on - strand, within RR42_RS29375at 1943.859 kb on + strand, within RR42_RS29375at 1943.859 kb on + strand, within RR42_RS29375at 1943.859 kb on + strand, within RR42_RS29375at 1943.860 kb on - strand, within RR42_RS29375at 1944.090 kb on + strandat 1944.091 kb on - strandat 1944.091 kb on - strandat 1944.231 kb on + strandat 1944.231 kb on + strandat 1944.232 kb on - strandat 1944.232 kb on - strandat 1944.232 kb on - strandat 1944.232 kb on - strandat 1944.402 kb on + strand, within RR42_RS29380at 1944.403 kb on - strand, within RR42_RS29380at 1944.427 kb on + strand, within RR42_RS29380at 1944.427 kb on + strand, within RR42_RS29380at 1944.428 kb on - strand, within RR42_RS29380at 1944.428 kb on - strand, within RR42_RS29380at 1944.872 kb on + strandat 1944.921 kb on + strandat 1944.921 kb on + strandat 1944.921 kb on + strandat 1944.922 kb on - strandat 1944.922 kb on - strandat 1944.935 kb on - strandat 1944.974 kb on - strandat 1944.974 kb on - strandat 1944.978 kb on + strandat 1944.979 kb on - strandat 1944.979 kb on - strandat 1944.984 kb on + strandat 1944.985 kb on - strandat 1944.985 kb on - strandat 1945.128 kb on + strandat 1945.128 kb on + strandat 1945.129 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml
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1,940,909 + RR42_RS29370 0.37 -0.2
1,941,032 - RR42_RS29370 0.47 -0.0
1,941,157 + RR42_RS29370 0.58 -0.1
1,941,157 + RR42_RS29370 0.58 +0.3
1,941,171 + RR42_RS29370 0.59 -1.5
1,941,171 + RR42_RS29370 0.59 +0.5
1,941,171 + RR42_RS29370 0.59 -1.2
1,941,310 + RR42_RS29370 0.70 +0.2
1,941,310 + RR42_RS29370 0.70 -1.3
1,941,412 + RR42_RS29370 0.78 +0.3
1,941,413 - RR42_RS29370 0.78 +2.4
1,941,509 - RR42_RS29370 0.86 +0.1
1,941,509 - RR42_RS29370 0.86 -0.1
1,941,526 - RR42_RS29370 0.88 +0.5
1,941,526 - RR42_RS29370 0.88 +0.2
1,941,798 + +0.3
1,941,798 + +0.8
1,941,798 + +0.9
1,941,798 + +1.3
1,941,799 - +0.7
1,941,799 - -0.6
1,941,910 - +0.2
1,941,910 - -0.4
1,941,921 + +0.5
1,941,921 + +0.6
1,941,921 + -0.4
1,941,922 - +0.9
1,941,922 - -1.3
1,941,922 - +0.4
1,941,942 + -0.4
1,941,943 - +0.5
1,942,009 - RR42_RS29375 0.12 +0.4
1,942,056 + RR42_RS29375 0.14 +0.8
1,942,057 - RR42_RS29375 0.14 +1.1
1,942,107 + RR42_RS29375 0.16 +0.7
1,942,107 + RR42_RS29375 0.16 +1.1
1,942,107 + RR42_RS29375 0.16 -1.0
1,942,107 + RR42_RS29375 0.16 -0.1
1,942,108 - RR42_RS29375 0.16 -1.1
1,942,206 + RR42_RS29375 0.20 +2.0
1,942,207 - RR42_RS29375 0.20 +1.9
1,942,207 - RR42_RS29375 0.20 +0.4
1,942,243 - RR42_RS29375 0.22 +0.1
1,942,297 - RR42_RS29375 0.24 +0.1
1,942,297 - RR42_RS29375 0.24 +0.7
1,942,297 - RR42_RS29375 0.24 -0.0
1,942,453 - RR42_RS29375 0.30 -0.7
1,942,453 - RR42_RS29375 0.30 +0.0
1,942,453 - RR42_RS29375 0.30 +0.3
1,942,453 - RR42_RS29375 0.30 +1.6
1,942,469 - RR42_RS29375 0.31 +0.2
1,942,478 - RR42_RS29375 0.31 +0.4
1,942,639 - RR42_RS29375 0.38 -0.9
1,942,639 - RR42_RS29375 0.38 +0.9
1,942,689 + RR42_RS29375 0.40 +0.8
1,942,689 + RR42_RS29375 0.40 -1.1
1,942,690 - RR42_RS29375 0.40 -0.3
1,942,833 + RR42_RS29375 0.45 -0.5
1,942,833 + RR42_RS29375 0.45 -0.5
1,942,833 + RR42_RS29375 0.45 -1.4
1,942,834 - RR42_RS29375 0.45 +0.8
1,942,834 - RR42_RS29375 0.45 -0.4
1,942,872 + RR42_RS29375 0.47 -0.1
1,942,873 - RR42_RS29375 0.47 +0.3
1,942,894 - RR42_RS29375 0.48 +0.0
1,943,007 + RR42_RS29375 0.52 +0.1
1,943,007 + RR42_RS29375 0.52 +0.9
1,943,007 + RR42_RS29375 0.52 +0.0
1,943,007 + RR42_RS29375 0.52 +0.2
1,943,007 + RR42_RS29375 0.52 -0.6
1,943,008 - RR42_RS29375 0.52 +0.7
1,943,008 - RR42_RS29375 0.52 +0.4
1,943,040 + RR42_RS29375 0.54 +0.0
1,943,053 + RR42_RS29375 0.54 -1.5
1,943,054 - RR42_RS29375 0.54 +1.0
1,943,086 - RR42_RS29375 0.56 +0.7
1,943,086 - RR42_RS29375 0.56 +0.6
1,943,194 + RR42_RS29375 0.60 -1.1
1,943,194 + RR42_RS29375 0.60 -0.4
1,943,194 + RR42_RS29375 0.60 -0.1
1,943,195 - RR42_RS29375 0.60 -0.1
1,943,195 - RR42_RS29375 0.60 -0.4
1,943,195 - RR42_RS29375 0.60 +0.1
1,943,196 + RR42_RS29375 0.60 -0.0
1,943,463 + RR42_RS29375 0.71 -0.9
1,943,463 + RR42_RS29375 0.71 -0.1
1,943,464 - RR42_RS29375 0.71 +1.5
1,943,464 - RR42_RS29375 0.71 +0.8
1,943,481 + RR42_RS29375 0.71 -0.6
1,943,532 + RR42_RS29375 0.74 -0.9
1,943,532 + RR42_RS29375 0.74 -1.0
1,943,532 + RR42_RS29375 0.74 -0.2
1,943,532 + RR42_RS29375 0.74 +0.2
1,943,532 + RR42_RS29375 0.74 -0.4
1,943,532 + RR42_RS29375 0.74 +2.2
1,943,532 + RR42_RS29375 0.74 +0.4
1,943,532 + RR42_RS29375 0.74 -0.2
1,943,533 - RR42_RS29375 0.74 +1.0
1,943,533 - RR42_RS29375 0.74 +0.6
1,943,589 + RR42_RS29375 0.76 -1.5
1,943,590 - RR42_RS29375 0.76 +0.2
1,943,590 - RR42_RS29375 0.76 -0.8
1,943,750 + RR42_RS29375 0.82 +0.1
1,943,788 + RR42_RS29375 0.84 -0.7
1,943,809 + RR42_RS29375 0.85 -0.5
1,943,810 - RR42_RS29375 0.85 +1.3
1,943,810 - RR42_RS29375 0.85 +1.3
1,943,811 + RR42_RS29375 0.85 -0.2
1,943,811 + RR42_RS29375 0.85 +0.3
1,943,811 + RR42_RS29375 0.85 +1.6
1,943,812 - RR42_RS29375 0.85 -0.3
1,943,812 - RR42_RS29375 0.85 -0.2
1,943,859 + RR42_RS29375 0.87 -0.2
1,943,859 + RR42_RS29375 0.87 -0.4
1,943,859 + RR42_RS29375 0.87 +0.8
1,943,860 - RR42_RS29375 0.87 -0.0
1,944,090 + -0.3
1,944,091 - +0.9
1,944,091 - -0.4
1,944,231 + +0.6
1,944,231 + -1.8
1,944,232 - -2.6
1,944,232 - -0.2
1,944,232 - +1.1
1,944,232 - -0.0
1,944,402 + RR42_RS29380 0.38 +0.1
1,944,403 - RR42_RS29380 0.38 +1.1
1,944,427 + RR42_RS29380 0.43 -1.1
1,944,427 + RR42_RS29380 0.43 +0.8
1,944,428 - RR42_RS29380 0.43 +1.2
1,944,428 - RR42_RS29380 0.43 +0.0
1,944,872 + +1.4
1,944,921 + +1.7
1,944,921 + -0.2
1,944,921 + -0.4
1,944,922 - -0.7
1,944,922 - +0.7
1,944,935 - +0.3
1,944,974 - +0.2
1,944,974 - +0.2
1,944,978 + +0.3
1,944,979 - +1.6
1,944,979 - -1.4
1,944,984 + +0.5
1,944,985 - +0.3
1,944,985 - +0.6
1,945,128 + -0.1
1,945,128 + -1.0
1,945,129 - -0.9

Or see this region's nucleotide sequence