Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29030 and RR42_RS29035 are separated by 132 nucleotides RR42_RS29035 and RR42_RS29040 are separated by 346 nucleotides RR42_RS29040 and RR42_RS29045 are separated by 177 nucleotides
RR42_RS29030: RR42_RS29030 - metal-dependent hydrolase, at 1,867,728 to 1,868,690
_RS29030
RR42_RS29035: RR42_RS29035 - acyl-CoA synthetase, at 1,868,823 to 1,870,505
_RS29035
RR42_RS29040: RR42_RS29040 - histidyl-tRNA synthetase, at 1,870,852 to 1,871,316
_RS29040
RR42_RS29045: RR42_RS29045 - esterase, at 1,871,494 to 1,872,456
_RS29045
Position (kb)
1868
1869
1870
1871 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1868.046 kb on + strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.406 kb on + strand, within RR42_RS29030 at 1868.406 kb on + strand, within RR42_RS29030 at 1868.407 kb on - strand, within RR42_RS29030 at 1868.623 kb on - strand at 1868.644 kb on + strand at 1868.645 kb on - strand at 1868.645 kb on - strand at 1868.645 kb on - strand at 1868.688 kb on + strand at 1868.688 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.785 kb on - strand at 1868.859 kb on + strand at 1868.859 kb on + strand at 1868.860 kb on - strand at 1868.860 kb on - strand at 1868.898 kb on - strand at 1868.898 kb on - strand at 1868.908 kb on + strand at 1868.961 kb on + strand at 1868.961 kb on + strand at 1868.961 kb on + strand at 1868.962 kb on - strand at 1868.962 kb on - strand at 1868.982 kb on + strand at 1868.982 kb on + strand at 1868.983 kb on - strand at 1869.067 kb on - strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.222 kb on + strand, within RR42_RS29035 at 1869.417 kb on + strand, within RR42_RS29035 at 1869.418 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.552 kb on + strand, within RR42_RS29035 at 1869.614 kb on + strand, within RR42_RS29035 at 1869.820 kb on - strand, within RR42_RS29035 at 1869.820 kb on - strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.882 kb on + strand, within RR42_RS29035 at 1869.883 kb on - strand, within RR42_RS29035 at 1869.883 kb on - strand, within RR42_RS29035 at 1869.883 kb on - strand, within RR42_RS29035 at 1869.884 kb on + strand, within RR42_RS29035 at 1869.884 kb on + strand, within RR42_RS29035 at 1869.885 kb on - strand, within RR42_RS29035 at 1869.885 kb on - strand, within RR42_RS29035 at 1869.885 kb on - strand, within RR42_RS29035 at 1870.098 kb on + strand, within RR42_RS29035 at 1870.143 kb on + strand, within RR42_RS29035 at 1870.191 kb on + strand, within RR42_RS29035 at 1870.192 kb on - strand, within RR42_RS29035 at 1870.233 kb on + strand, within RR42_RS29035 at 1870.234 kb on - strand, within RR42_RS29035 at 1870.269 kb on + strand, within RR42_RS29035 at 1870.269 kb on + strand, within RR42_RS29035 at 1870.269 kb on + strand, within RR42_RS29035 at 1870.270 kb on - strand, within RR42_RS29035 at 1870.270 kb on - strand, within RR42_RS29035 at 1870.419 kb on + strand at 1870.419 kb on + strand at 1870.420 kb on - strand at 1870.421 kb on + strand at 1870.421 kb on + strand at 1870.421 kb on + strand at 1870.422 kb on - strand at 1870.422 kb on - strand at 1870.422 kb on - strand at 1870.422 kb on - strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.601 kb on + strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.603 kb on + strand at 1870.604 kb on - strand at 1870.841 kb on + strand at 1870.841 kb on + strand at 1870.842 kb on - strand at 1870.842 kb on - strand at 1870.845 kb on + strand at 1870.846 kb on - strand at 1870.846 kb on - strand at 1870.852 kb on - strand at 1870.934 kb on + strand, within RR42_RS29040 at 1871.048 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.432 kb on + strand at 1871.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,867,827 + RR42_RS29030 0.10 +1.3 1,867,827 + RR42_RS29030 0.10 +0.3 1,867,827 + RR42_RS29030 0.10 -0.4 1,867,827 + RR42_RS29030 0.10 +0.5 1,867,828 - RR42_RS29030 0.10 -0.2 1,867,828 - RR42_RS29030 0.10 -0.8 1,867,828 - RR42_RS29030 0.10 +0.1 1,867,980 + RR42_RS29030 0.26 -0.6 1,867,980 + RR42_RS29030 0.26 -0.3 1,868,046 + RR42_RS29030 0.33 +0.9 1,868,047 - RR42_RS29030 0.33 +1.7 1,868,047 - RR42_RS29030 0.33 +0.4 1,868,047 - RR42_RS29030 0.33 -0.7 1,868,047 - RR42_RS29030 0.33 +0.6 1,868,406 + RR42_RS29030 0.70 -0.6 1,868,406 + RR42_RS29030 0.70 -0.1 1,868,407 - RR42_RS29030 0.71 -0.9 1,868,623 - -0.4 1,868,644 + +0.3 1,868,645 - +1.3 1,868,645 - +1.0 1,868,645 - -1.9 1,868,688 + +1.3 1,868,688 + +0.8 1,868,784 + -0.9 1,868,784 + +0.5 1,868,784 + +1.8 1,868,784 + +0.3 1,868,785 - +2.3 1,868,859 + -0.1 1,868,859 + -0.6 1,868,860 - +0.3 1,868,860 - +0.3 1,868,898 - -1.2 1,868,898 - +0.3 1,868,908 + -0.1 1,868,961 + +0.6 1,868,961 + +0.2 1,868,961 + +0.7 1,868,962 - -1.4 1,868,962 - +1.0 1,868,982 + -1.8 1,868,982 + -0.4 1,868,983 - +0.1 1,869,067 - RR42_RS29035 0.14 +0.5 1,869,199 + RR42_RS29035 0.22 +0.3 1,869,199 + RR42_RS29035 0.22 +0.4 1,869,199 + RR42_RS29035 0.22 -0.5 1,869,199 + RR42_RS29035 0.22 -0.6 1,869,222 + RR42_RS29035 0.24 +0.7 1,869,417 + RR42_RS29035 0.35 -1.1 1,869,418 - RR42_RS29035 0.35 +0.8 1,869,478 - RR42_RS29035 0.39 +0.2 1,869,478 - RR42_RS29035 0.39 +2.5 1,869,478 - RR42_RS29035 0.39 -0.1 1,869,552 + RR42_RS29035 0.43 +0.5 1,869,614 + RR42_RS29035 0.47 +0.8 1,869,820 - RR42_RS29035 0.59 -0.1 1,869,820 - RR42_RS29035 0.59 +0.3 1,869,882 + RR42_RS29035 0.63 +0.1 1,869,882 + RR42_RS29035 0.63 +0.0 1,869,882 + RR42_RS29035 0.63 -0.1 1,869,882 + RR42_RS29035 0.63 +1.1 1,869,882 + RR42_RS29035 0.63 +0.5 1,869,882 + RR42_RS29035 0.63 +0.7 1,869,882 + RR42_RS29035 0.63 -0.6 1,869,882 + RR42_RS29035 0.63 -0.9 1,869,882 + RR42_RS29035 0.63 +0.4 1,869,883 - RR42_RS29035 0.63 +1.0 1,869,883 - RR42_RS29035 0.63 -2.3 1,869,883 - RR42_RS29035 0.63 +0.4 1,869,884 + RR42_RS29035 0.63 +0.2 1,869,884 + RR42_RS29035 0.63 +0.8 1,869,885 - RR42_RS29035 0.63 +1.1 1,869,885 - RR42_RS29035 0.63 -0.3 1,869,885 - RR42_RS29035 0.63 +0.2 1,870,098 + RR42_RS29035 0.76 +1.0 1,870,143 + RR42_RS29035 0.78 -0.2 1,870,191 + RR42_RS29035 0.81 +0.9 1,870,192 - RR42_RS29035 0.81 +0.5 1,870,233 + RR42_RS29035 0.84 -0.0 1,870,234 - RR42_RS29035 0.84 -0.2 1,870,269 + RR42_RS29035 0.86 +0.4 1,870,269 + RR42_RS29035 0.86 +0.5 1,870,269 + RR42_RS29035 0.86 +0.5 1,870,270 - RR42_RS29035 0.86 -0.0 1,870,270 - RR42_RS29035 0.86 -0.9 1,870,419 + -0.5 1,870,419 + -0.2 1,870,420 - +0.2 1,870,421 + -0.7 1,870,421 + +0.3 1,870,421 + +0.4 1,870,422 - -0.7 1,870,422 - -0.7 1,870,422 - -0.2 1,870,422 - -0.9 1,870,599 + +1.3 1,870,599 + +0.6 1,870,599 + +0.1 1,870,599 + -0.5 1,870,599 + -0.2 1,870,599 + +1.3 1,870,600 - +0.1 1,870,600 - +0.5 1,870,600 - +2.0 1,870,600 - +1.1 1,870,600 - +0.3 1,870,601 + +0.6 1,870,602 - +0.2 1,870,602 - +1.3 1,870,602 - +0.5 1,870,603 + +0.2 1,870,604 - -0.0 1,870,841 + +1.7 1,870,841 + +0.2 1,870,842 - -2.3 1,870,842 - -0.8 1,870,845 + +0.5 1,870,846 - -0.3 1,870,846 - -1.6 1,870,852 - +0.8 1,870,934 + RR42_RS29040 0.18 +0.3 1,871,048 + RR42_RS29040 0.42 +0.3 1,871,186 + RR42_RS29040 0.72 +0.1 1,871,186 + RR42_RS29040 0.72 -0.0 1,871,187 - RR42_RS29040 0.72 -0.6 1,871,187 - RR42_RS29040 0.72 -0.3 1,871,432 + +2.1 1,871,500 - -1.1
Or see this region's nucleotide sequence