Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27720 and RR42_RS27725 are separated by 5 nucleotides RR42_RS27725 and RR42_RS27730 are separated by 102 nucleotides RR42_RS27730 and RR42_RS27735 are separated by 229 nucleotides
RR42_RS27720: RR42_RS27720 - MFS transporter, at 1,582,574 to 1,583,896
_RS27720
RR42_RS27725: RR42_RS27725 - GntR family transcriptional regulator, at 1,583,902 to 1,584,612
_RS27725
RR42_RS27730: RR42_RS27730 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,584,715 to 1,585,674
_RS27730
RR42_RS27735: RR42_RS27735 - GntR family transcriptional regulator, at 1,585,904 to 1,586,632
_RS27735
Position (kb)
1584
1585
1586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1583.723 kb on + strand, within RR42_RS27720 at 1583.725 kb on + strand, within RR42_RS27720 at 1583.725 kb on + strand, within RR42_RS27720 at 1583.725 kb on + strand, within RR42_RS27720 at 1583.816 kb on + strand at 1583.816 kb on + strand at 1583.816 kb on + strand at 1583.817 kb on - strand at 1584.292 kb on + strand, within RR42_RS27725 at 1584.537 kb on + strand, within RR42_RS27725 at 1584.539 kb on + strand, within RR42_RS27725 at 1584.540 kb on - strand, within RR42_RS27725 at 1584.540 kb on - strand, within RR42_RS27725 at 1584.666 kb on + strand at 1584.666 kb on + strand at 1584.667 kb on - strand at 1584.717 kb on - strand at 1584.803 kb on + strand at 1584.890 kb on + strand, within RR42_RS27730 at 1584.932 kb on + strand, within RR42_RS27730 at 1584.932 kb on + strand, within RR42_RS27730 at 1584.933 kb on - strand, within RR42_RS27730 at 1584.933 kb on - strand, within RR42_RS27730 at 1584.934 kb on + strand, within RR42_RS27730 at 1584.934 kb on + strand, within RR42_RS27730 at 1584.935 kb on - strand, within RR42_RS27730 at 1584.935 kb on - strand, within RR42_RS27730 at 1584.935 kb on - strand, within RR42_RS27730 at 1585.145 kb on + strand, within RR42_RS27730 at 1585.146 kb on - strand, within RR42_RS27730 at 1585.371 kb on - strand, within RR42_RS27730 at 1585.409 kb on + strand, within RR42_RS27730 at 1585.409 kb on + strand, within RR42_RS27730 at 1585.409 kb on + strand, within RR42_RS27730 at 1585.409 kb on + strand, within RR42_RS27730 at 1585.410 kb on - strand, within RR42_RS27730 at 1585.410 kb on - strand, within RR42_RS27730 at 1585.410 kb on - strand, within RR42_RS27730 at 1585.416 kb on + strand, within RR42_RS27730 at 1585.417 kb on - strand, within RR42_RS27730 at 1585.434 kb on + strand, within RR42_RS27730 at 1585.434 kb on + strand, within RR42_RS27730 at 1585.434 kb on + strand, within RR42_RS27730 at 1585.435 kb on - strand, within RR42_RS27730 at 1585.619 kb on + strand at 1585.839 kb on + strand at 1585.839 kb on + strand at 1585.840 kb on - strand at 1586.064 kb on + strand, within RR42_RS27735 at 1586.064 kb on + strand, within RR42_RS27735 at 1586.065 kb on - strand, within RR42_RS27735 at 1586.065 kb on - strand, within RR42_RS27735 at 1586.172 kb on - strand, within RR42_RS27735 at 1586.224 kb on + strand, within RR42_RS27735 at 1586.224 kb on + strand, within RR42_RS27735 at 1586.225 kb on - strand, within RR42_RS27735 at 1586.226 kb on + strand, within RR42_RS27735 at 1586.226 kb on + strand, within RR42_RS27735 at 1586.226 kb on + strand, within RR42_RS27735 at 1586.226 kb on + strand, within RR42_RS27735 at 1586.226 kb on + strand, within RR42_RS27735 at 1586.227 kb on - strand, within RR42_RS27735 at 1586.227 kb on - strand, within RR42_RS27735 at 1586.336 kb on + strand, within RR42_RS27735 at 1586.336 kb on + strand, within RR42_RS27735 at 1586.337 kb on - strand, within RR42_RS27735 at 1586.337 kb on - strand, within RR42_RS27735 at 1586.538 kb on - strand, within RR42_RS27735 at 1586.604 kb on - strand at 1586.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,583,723 + RR42_RS27720 0.87 +0.2 1,583,725 + RR42_RS27720 0.87 -0.6 1,583,725 + RR42_RS27720 0.87 +0.1 1,583,725 + RR42_RS27720 0.87 +0.4 1,583,816 + -0.3 1,583,816 + +0.6 1,583,816 + +0.1 1,583,817 - -1.4 1,584,292 + RR42_RS27725 0.55 +0.3 1,584,537 + RR42_RS27725 0.89 -0.4 1,584,539 + RR42_RS27725 0.90 -1.1 1,584,540 - RR42_RS27725 0.90 -1.5 1,584,540 - RR42_RS27725 0.90 -0.5 1,584,666 + +0.5 1,584,666 + -1.1 1,584,667 - +0.8 1,584,717 - +2.1 1,584,803 + +0.5 1,584,890 + RR42_RS27730 0.18 +0.1 1,584,932 + RR42_RS27730 0.23 +0.4 1,584,932 + RR42_RS27730 0.23 +0.4 1,584,933 - RR42_RS27730 0.23 +0.1 1,584,933 - RR42_RS27730 0.23 +0.2 1,584,934 + RR42_RS27730 0.23 +0.2 1,584,934 + RR42_RS27730 0.23 -0.5 1,584,935 - RR42_RS27730 0.23 -0.9 1,584,935 - RR42_RS27730 0.23 -0.2 1,584,935 - RR42_RS27730 0.23 -1.0 1,585,145 + RR42_RS27730 0.45 +0.0 1,585,146 - RR42_RS27730 0.45 -0.5 1,585,371 - RR42_RS27730 0.68 +0.5 1,585,409 + RR42_RS27730 0.72 -0.0 1,585,409 + RR42_RS27730 0.72 -0.2 1,585,409 + RR42_RS27730 0.72 +0.8 1,585,409 + RR42_RS27730 0.72 -1.4 1,585,410 - RR42_RS27730 0.72 +0.3 1,585,410 - RR42_RS27730 0.72 -0.2 1,585,410 - RR42_RS27730 0.72 +0.4 1,585,416 + RR42_RS27730 0.73 +0.3 1,585,417 - RR42_RS27730 0.73 +0.3 1,585,434 + RR42_RS27730 0.75 +0.9 1,585,434 + RR42_RS27730 0.75 +0.4 1,585,434 + RR42_RS27730 0.75 -0.3 1,585,435 - RR42_RS27730 0.75 -0.3 1,585,619 + +0.2 1,585,839 + +1.2 1,585,839 + -1.0 1,585,840 - -0.8 1,586,064 + RR42_RS27735 0.22 +0.8 1,586,064 + RR42_RS27735 0.22 +0.2 1,586,065 - RR42_RS27735 0.22 +0.3 1,586,065 - RR42_RS27735 0.22 +0.0 1,586,172 - RR42_RS27735 0.37 -0.0 1,586,224 + RR42_RS27735 0.44 -0.2 1,586,224 + RR42_RS27735 0.44 -0.1 1,586,225 - RR42_RS27735 0.44 +0.4 1,586,226 + RR42_RS27735 0.44 -1.7 1,586,226 + RR42_RS27735 0.44 +0.6 1,586,226 + RR42_RS27735 0.44 +1.5 1,586,226 + RR42_RS27735 0.44 +0.2 1,586,226 + RR42_RS27735 0.44 +0.5 1,586,227 - RR42_RS27735 0.44 +0.1 1,586,227 - RR42_RS27735 0.44 +0.4 1,586,336 + RR42_RS27735 0.59 +0.7 1,586,336 + RR42_RS27735 0.59 +0.0 1,586,337 - RR42_RS27735 0.59 -2.6 1,586,337 - RR42_RS27735 0.59 +0.5 1,586,538 - RR42_RS27735 0.87 -0.2 1,586,604 - +0.1 1,586,604 - +0.5
Or see this region's nucleotide sequence