Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27390 and RR42_RS27395 overlap by 4 nucleotides RR42_RS27395 and RR42_RS27400 are separated by 4 nucleotides
RR42_RS27390: RR42_RS27390 - acetylpolyamine aminohydrolase, at 1,496,390 to 1,497,418
_RS27390
RR42_RS27395: RR42_RS27395 - GntR family transcriptional regulator, at 1,497,415 to 1,498,140
_RS27395
RR42_RS27400: RR42_RS27400 - membrane protein, at 1,498,145 to 1,499,095
_RS27400
Position (kb)
1497
1498
1499 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1496.649 kb on + strand, within RR42_RS27390 at 1496.694 kb on + strand, within RR42_RS27390 at 1496.911 kb on - strand, within RR42_RS27390 at 1496.937 kb on + strand, within RR42_RS27390 at 1496.937 kb on + strand, within RR42_RS27390 at 1496.938 kb on - strand, within RR42_RS27390 at 1497.117 kb on + strand, within RR42_RS27390 at 1497.118 kb on - strand, within RR42_RS27390 at 1497.599 kb on + strand, within RR42_RS27395 at 1497.668 kb on + strand, within RR42_RS27395 at 1497.668 kb on + strand, within RR42_RS27395 at 1497.668 kb on + strand, within RR42_RS27395 at 1497.669 kb on - strand, within RR42_RS27395 at 1498.146 kb on + strand at 1498.163 kb on + strand at 1498.300 kb on + strand, within RR42_RS27400 at 1498.301 kb on - strand, within RR42_RS27400 at 1498.332 kb on + strand, within RR42_RS27400 at 1498.333 kb on - strand, within RR42_RS27400 at 1498.333 kb on - strand, within RR42_RS27400 at 1498.581 kb on + strand, within RR42_RS27400 at 1498.759 kb on - strand, within RR42_RS27400 at 1498.786 kb on + strand, within RR42_RS27400 at 1498.786 kb on + strand, within RR42_RS27400 at 1498.787 kb on - strand, within RR42_RS27400 at 1498.787 kb on - strand, within RR42_RS27400 at 1498.932 kb on + strand, within RR42_RS27400 at 1498.933 kb on - strand, within RR42_RS27400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,496,649 + RR42_RS27390 0.25 +0.5 1,496,694 + RR42_RS27390 0.30 -0.3 1,496,911 - RR42_RS27390 0.51 +0.0 1,496,937 + RR42_RS27390 0.53 -0.6 1,496,937 + RR42_RS27390 0.53 -1.3 1,496,938 - RR42_RS27390 0.53 -1.7 1,497,117 + RR42_RS27390 0.71 -0.2 1,497,118 - RR42_RS27390 0.71 -1.4 1,497,599 + RR42_RS27395 0.25 +1.3 1,497,668 + RR42_RS27395 0.35 +0.4 1,497,668 + RR42_RS27395 0.35 +0.4 1,497,668 + RR42_RS27395 0.35 +0.6 1,497,669 - RR42_RS27395 0.35 +1.5 1,498,146 + +0.0 1,498,163 + -0.4 1,498,300 + RR42_RS27400 0.16 -0.4 1,498,301 - RR42_RS27400 0.16 -0.6 1,498,332 + RR42_RS27400 0.20 +0.8 1,498,333 - RR42_RS27400 0.20 -0.3 1,498,333 - RR42_RS27400 0.20 -0.7 1,498,581 + RR42_RS27400 0.46 +1.0 1,498,759 - RR42_RS27400 0.65 -3.1 1,498,786 + RR42_RS27400 0.67 -1.1 1,498,786 + RR42_RS27400 0.67 -1.0 1,498,787 - RR42_RS27400 0.68 -0.0 1,498,787 - RR42_RS27400 0.68 +0.1 1,498,932 + RR42_RS27400 0.83 -0.1 1,498,933 - RR42_RS27400 0.83 -0.1
Or see this region's nucleotide sequence