Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27270 and RR42_RS27275 are separated by 78 nucleotides RR42_RS27275 and RR42_RS27280 are separated by 84 nucleotides
RR42_RS27270: RR42_RS27270 - NADP-dependent oxidoreductase, at 1,471,057 to 1,472,088
_RS27270
RR42_RS27275: RR42_RS27275 - alpha-dehydro-beta-deoxy-D-glucarate aldolase, at 1,472,167 to 1,472,934
_RS27275
RR42_RS27280: RR42_RS27280 - hypothetical protein, at 1,473,019 to 1,473,999
_RS27280
Position (kb) 1472
1473 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1471.430 kb on + strand, within RR42_RS27270 at 1471.430 kb on + strand, within RR42_RS27270 at 1471.466 kb on + strand, within RR42_RS27270 at 1471.467 kb on - strand, within RR42_RS27270 at 1471.541 kb on + strand, within RR42_RS27270 at 1471.541 kb on + strand, within RR42_RS27270 at 1471.541 kb on + strand, within RR42_RS27270 at 1471.542 kb on - strand, within RR42_RS27270 at 1471.709 kb on + strand, within RR42_RS27270 at 1471.709 kb on + strand, within RR42_RS27270 at 1471.724 kb on + strand, within RR42_RS27270 at 1471.725 kb on - strand, within RR42_RS27270 at 1471.739 kb on + strand, within RR42_RS27270 at 1471.739 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.916 kb on + strand, within RR42_RS27270 at 1471.917 kb on - strand, within RR42_RS27270 at 1471.917 kb on - strand, within RR42_RS27270 at 1471.917 kb on - strand, within RR42_RS27270 at 1472.168 kb on + strand at 1472.168 kb on + strand at 1472.268 kb on - strand, within RR42_RS27275 at 1472.268 kb on - strand at 1472.520 kb on + strand, within RR42_RS27275 at 1472.521 kb on - strand, within RR42_RS27275 at 1472.645 kb on + strand, within RR42_RS27275 at 1472.646 kb on - strand, within RR42_RS27275 at 1472.976 kb on + strand at 1472.976 kb on + strand at 1472.977 kb on - strand at 1472.977 kb on - strand at 1472.977 kb on - strand at 1472.977 kb on - strand at 1472.977 kb on - strand at 1473.176 kb on - strand, within RR42_RS27280 at 1473.356 kb on + strand, within RR42_RS27280 at 1473.614 kb on + strand, within RR42_RS27280 at 1473.681 kb on - strand, within RR42_RS27280 at 1473.731 kb on + strand, within RR42_RS27280 at 1473.882 kb on - strand, within RR42_RS27280 at 1473.882 kb on - strand, within RR42_RS27280 at 1473.882 kb on - strand, within RR42_RS27280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,471,430 + RR42_RS27270 0.36 -0.5 1,471,430 + RR42_RS27270 0.36 -0.9 1,471,466 + RR42_RS27270 0.40 +0.1 1,471,467 - RR42_RS27270 0.40 -0.3 1,471,541 + RR42_RS27270 0.47 +0.1 1,471,541 + RR42_RS27270 0.47 -0.0 1,471,541 + RR42_RS27270 0.47 -0.2 1,471,542 - RR42_RS27270 0.47 +0.2 1,471,709 + RR42_RS27270 0.63 -0.7 1,471,709 + RR42_RS27270 0.63 +0.1 1,471,724 + RR42_RS27270 0.65 -0.3 1,471,725 - RR42_RS27270 0.65 -0.5 1,471,739 + RR42_RS27270 0.66 -0.2 1,471,739 + RR42_RS27270 0.66 +0.4 1,471,916 + RR42_RS27270 0.83 -0.1 1,471,916 + RR42_RS27270 0.83 -0.4 1,471,916 + RR42_RS27270 0.83 -1.2 1,471,916 + RR42_RS27270 0.83 +0.7 1,471,916 + RR42_RS27270 0.83 +0.0 1,471,916 + RR42_RS27270 0.83 +0.8 1,471,917 - RR42_RS27270 0.83 +0.2 1,471,917 - RR42_RS27270 0.83 +0.0 1,471,917 - RR42_RS27270 0.83 -0.5 1,472,168 + +1.0 1,472,168 + +1.2 1,472,268 - RR42_RS27275 0.13 +0.1 1,472,268 - +2.3 1,472,520 + RR42_RS27275 0.46 +0.9 1,472,521 - RR42_RS27275 0.46 -1.9 1,472,645 + RR42_RS27275 0.62 +0.7 1,472,646 - RR42_RS27275 0.62 +0.5 1,472,976 + -1.8 1,472,976 + -0.9 1,472,977 - +0.3 1,472,977 - +0.2 1,472,977 - -0.1 1,472,977 - +0.0 1,472,977 - +0.7 1,473,176 - RR42_RS27280 0.16 -1.5 1,473,356 + RR42_RS27280 0.34 -0.0 1,473,614 + RR42_RS27280 0.61 -1.2 1,473,681 - RR42_RS27280 0.67 +0.6 1,473,731 + RR42_RS27280 0.73 -0.7 1,473,882 - RR42_RS27280 0.88 +0.5 1,473,882 - RR42_RS27280 0.88 -0.8 1,473,882 - RR42_RS27280 0.88 -1.8
Or see this region's nucleotide sequence