Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27060 and RR42_RS27065 are separated by 393 nucleotides RR42_RS27065 and RR42_RS27070 are separated by 27 nucleotides RR42_RS27070 and RR42_RS27075 are separated by 483 nucleotides
RR42_RS27060: RR42_RS27060 - cation transporter, at 1,424,104 to 1,425,243
_RS27060
RR42_RS27065: RR42_RS27065 - hypothetical protein, at 1,425,637 to 1,425,993
_RS27065
RR42_RS27070: RR42_RS27070 - membrane protein, at 1,426,021 to 1,426,725
_RS27070
RR42_RS27075: RR42_RS27075 - MFS transporter, at 1,427,209 to 1,428,441
_RS27075
Position (kb)
1426
1427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1425.123 kb on + strand, within RR42_RS27060 at 1425.124 kb on - strand, within RR42_RS27060 at 1425.189 kb on + strand at 1425.881 kb on + strand, within RR42_RS27065 at 1425.993 kb on + strand at 1426.150 kb on + strand, within RR42_RS27070 at 1426.150 kb on + strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.268 kb on - strand, within RR42_RS27070 at 1426.284 kb on - strand, within RR42_RS27070 at 1426.459 kb on - strand, within RR42_RS27070 at 1426.834 kb on - strand at 1426.834 kb on - strand at 1426.951 kb on - strand at 1427.004 kb on - strand at 1427.004 kb on - strand at 1427.085 kb on + strand at 1427.085 kb on + strand at 1427.086 kb on - strand at 1427.086 kb on - strand at 1427.122 kb on - strand at 1427.207 kb on - strand at 1427.391 kb on - strand, within RR42_RS27075 at 1427.691 kb on - strand, within RR42_RS27075 at 1427.693 kb on + strand, within RR42_RS27075 at 1427.693 kb on + strand, within RR42_RS27075 at 1427.694 kb on - strand, within RR42_RS27075 at 1427.694 kb on - strand at 1427.694 kb on - strand, within RR42_RS27075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,425,123 + RR42_RS27060 0.89 -1.1 1,425,124 - RR42_RS27060 0.89 +0.4 1,425,189 + -0.5 1,425,881 + RR42_RS27065 0.68 -0.1 1,425,993 + +0.4 1,426,150 + RR42_RS27070 0.18 -0.7 1,426,150 + RR42_RS27070 0.18 +1.1 1,426,151 - RR42_RS27070 0.18 +0.4 1,426,151 - RR42_RS27070 0.18 -1.1 1,426,151 - RR42_RS27070 0.18 -2.1 1,426,268 - RR42_RS27070 0.35 -3.1 1,426,284 - RR42_RS27070 0.37 -2.1 1,426,459 - RR42_RS27070 0.62 -1.7 1,426,834 - +0.0 1,426,834 - +1.9 1,426,951 - -0.1 1,427,004 - +0.4 1,427,004 - -0.7 1,427,085 + +0.5 1,427,085 + +0.4 1,427,086 - -2.7 1,427,086 - -1.7 1,427,122 - -0.2 1,427,207 - -1.1 1,427,391 - RR42_RS27075 0.15 +0.0 1,427,691 - RR42_RS27075 0.39 -1.4 1,427,693 + RR42_RS27075 0.39 +0.2 1,427,693 + RR42_RS27075 0.39 -2.4 1,427,694 - RR42_RS27075 0.39 -0.4 1,427,694 - +2.3 1,427,694 - RR42_RS27075 0.39 -0.0
Or see this region's nucleotide sequence